This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture te...This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.展开更多
Sustainable production of Dendrobium officinale,a valuable medicinal and edible plant,faces challenges due to overexploitation.This study investigated the effects of exogenous 2,3-butanediol(BDO)-containing fermentati...Sustainable production of Dendrobium officinale,a valuable medicinal and edible plant,faces challenges due to overexploitation.This study investigated the effects of exogenous 2,3-butanediol(BDO)-containing fermentation broth,γ-aminobutyric acid(GABA),and melatonin(MEL)on the growth and metabolism of D.officinale protocorm-like bodies(PLBs).All exogenous additives markedly boosted the biomass and polysaccharide,alkaloid,ascorbic acid,GABA,and MEL contents,accompanied by an enhancement in total antioxidant capacity in the PLBs.Among the additives,a combination of GABA and BDO-containing broth produced the largest in-crease in the production of alkaloids,anthocyanins,and GABA at 122.49%,97.60%,and 29.15%,respectively,compared with the control group.A combination of GABA and MEL,resulted in the highest increase in the contents of polysaccharides,MEL and total antioxidant capacity,which increased by 39.06%,415.36%,and 49.21%,respectively,compared with the control.Exogenous additives increased the contents of endogenous hormones,chlorophyll,C and N elements,antioxidant substances,as well as antioxidant oxidase activities,indicating that the improved yield and quality of PLBs probably occurred via the synergistic action of hormone balancing,activation of antioxidant defense,and promotion of carbon and nitrogen assimilation by the additives.This study established a high-yield production system for D.officinale PLBs rich in bioactive compounds via the application of exogenous additives.At the same time,it also provided theoretical and technical support for the large-scale production of PLBs,which is of great significance for ensuring the sustainable supply of high-quality D.officinale raw materials used in functional food.展开更多
以铁皮石斛Dendrobium officinale的茎尖为外植体,在特定条件下诱导产生拟原球茎,进一步培养形成幼苗。结果表明:原球茎最优诱导培养基为1/2MS(Murashige and Skoog)+0.5mg·L-16-苄基嘌呤(6-BA)+0.05mg·L-1萘乙酸(NAA)+0.1mg&...以铁皮石斛Dendrobium officinale的茎尖为外植体,在特定条件下诱导产生拟原球茎,进一步培养形成幼苗。结果表明:原球茎最优诱导培养基为1/2MS(Murashige and Skoog)+0.5mg·L-16-苄基嘌呤(6-BA)+0.05mg·L-1萘乙酸(NAA)+0.1mg·L-1激动素(KT)+1.0g·L-1水解乳蛋白(LH)。复合添加物的试验表明,土豆汁对拟原球茎(PLBs)的增殖有很好的促进作用。对铁皮石斛拟原球茎的解剖学研究表明:拟原球茎是由位于顶端分生组织下的薄壁细胞脱分化形成的胚性细胞发育而来。外植体接种后20d左右外植体基部开始膨大,40d左右出现肉眼可见的乳白色突起,这些突起逐渐增大,成为圆形至卵圆形的乳白色拟原球茎。在条件适宜的拟原球茎增殖培养基上继续培养,拟原球茎边缘的薄壁细胞继续脱分化,进一步发育形成球形的细胞团,可继续形成突起,使拟原球茎不断增殖。展开更多
基金supported by the Natural Science Foundation of Guangdong Province (05300848)Fok Ying Tung Education Foundation (104031)the National Natural Science Foundation of China(30800758)
文摘This research aims at developing a plant regeneration system from leaf and petiole explants of Anthurium andraeanum Hort., thereby establish a foundation for mass production and transformation. Using tissue culture technique, the conditions for callus induction, protocorm-like body （PLB） formation and plant regeneration from leaf explants and petiole of A. andraeanum, such as basal medium and plant growth regulator, were investigated. Totipotent callus was induced on a 1/2-strength MS medium containing 0.90 μmol L^-1 2,4-dichlorophenoxyacetic acid （2,4-D） and 8.88μmol L^-1 N6-benzyladenine （BA）. The callus exhibited complete hormone autonomy for growth and differentiation of PLBs. This callus proliferated well and was maintained by subculturing on 1/2 MS medium containing 0.90 μmol L^-1 2,4-D and 4.44 μmol L^-1 BA. On average, 8 protocorm-like bodies could be obtained from a piece of 4 mm callus after being transferred to the 1/2 MS medium with 4.44 μmol L^-1 BA after 8 wk of culture. The regenerated PLBs formed shoots and roots on 1/2 MS medium. After 24 wk of culture on these medium, well-developed plantlets for potting were produced. An efficient micropropagation method was established for indirect PLB formation and plant regeneration from leaf and petiole ofA. andraeanum.
基金supported by the Yunnan Provincial Science and Technology Department,China,under the Science and Technology Planning Project(Grant No.202302AE090021).
文摘Sustainable production of Dendrobium officinale,a valuable medicinal and edible plant,faces challenges due to overexploitation.This study investigated the effects of exogenous 2,3-butanediol(BDO)-containing fermentation broth,γ-aminobutyric acid(GABA),and melatonin(MEL)on the growth and metabolism of D.officinale protocorm-like bodies(PLBs).All exogenous additives markedly boosted the biomass and polysaccharide,alkaloid,ascorbic acid,GABA,and MEL contents,accompanied by an enhancement in total antioxidant capacity in the PLBs.Among the additives,a combination of GABA and BDO-containing broth produced the largest in-crease in the production of alkaloids,anthocyanins,and GABA at 122.49%,97.60%,and 29.15%,respectively,compared with the control group.A combination of GABA and MEL,resulted in the highest increase in the contents of polysaccharides,MEL and total antioxidant capacity,which increased by 39.06%,415.36%,and 49.21%,respectively,compared with the control.Exogenous additives increased the contents of endogenous hormones,chlorophyll,C and N elements,antioxidant substances,as well as antioxidant oxidase activities,indicating that the improved yield and quality of PLBs probably occurred via the synergistic action of hormone balancing,activation of antioxidant defense,and promotion of carbon and nitrogen assimilation by the additives.This study established a high-yield production system for D.officinale PLBs rich in bioactive compounds via the application of exogenous additives.At the same time,it also provided theoretical and technical support for the large-scale production of PLBs,which is of great significance for ensuring the sustainable supply of high-quality D.officinale raw materials used in functional food.
文摘以铁皮石斛Dendrobium officinale的茎尖为外植体,在特定条件下诱导产生拟原球茎,进一步培养形成幼苗。结果表明:原球茎最优诱导培养基为1/2MS(Murashige and Skoog)+0.5mg·L-16-苄基嘌呤(6-BA)+0.05mg·L-1萘乙酸(NAA)+0.1mg·L-1激动素(KT)+1.0g·L-1水解乳蛋白(LH)。复合添加物的试验表明,土豆汁对拟原球茎(PLBs)的增殖有很好的促进作用。对铁皮石斛拟原球茎的解剖学研究表明:拟原球茎是由位于顶端分生组织下的薄壁细胞脱分化形成的胚性细胞发育而来。外植体接种后20d左右外植体基部开始膨大,40d左右出现肉眼可见的乳白色突起,这些突起逐渐增大,成为圆形至卵圆形的乳白色拟原球茎。在条件适宜的拟原球茎增殖培养基上继续培养,拟原球茎边缘的薄壁细胞继续脱分化,进一步发育形成球形的细胞团,可继续形成突起,使拟原球茎不断增殖。
