In the training studio of the School of Architecture and Engineering at Xizang Vocational Technical College,Lhasa,Gyatso,a senior artist specializing in Tibetan-style architectural decorative painting,bent over to dem...In the training studio of the School of Architecture and Engineering at Xizang Vocational Technical College,Lhasa,Gyatso,a senior artist specializing in Tibetan-style architectural decorative painting,bent over to demonstrate a student how to paint.His left hand steadied the painting board,while his right hand held a brush pen to present the Tibetan-style painting technique of"blurring"-dipping the brush lightly into the pigment,spreading it from dark to light on the paper.The eight-petaled lotus,a symbol of auspiciousness,gradually took shape,blossoming with layers of vitality.展开更多
Conversation B Susie:I know.Pencils are also better for drawing.You can make lines lighter or darker.Sam:But you have to sharpen pencils or add lead to them.Susie:You do.But pencils last longer than pens.Pen ink can d...Conversation B Susie:I know.Pencils are also better for drawing.You can make lines lighter or darker.Sam:But you have to sharpen pencils or add lead to them.Susie:You do.But pencils last longer than pens.Pen ink can dry out,but pencil lead doesn’t.Sam:But words look neater when you write in pen.Susie:They do.So,which pen do you want?Sam:One with blue ink,thanks.展开更多
一、阅读理解[核心素养·文化意识](2024·贵州铜仁一模) The brush pen, one of the “Four Treasures of the Study”, was invented thou-sands of years ago. However, the oldest pen was just a stick or a piece of bamb...一、阅读理解[核心素养·文化意识](2024·贵州铜仁一模) The brush pen, one of the “Four Treasures of the Study”, was invented thou-sands of years ago. However, the oldest pen was just a stick or a piece of bamboo. It was difficult for people to write well on thin silk.展开更多
Both the Todo Alphabet written with bambuu pen that is kept by Burintögs Khagai in County Zhaosu and the one written with soft brush that is kept by Namrjil Vjar have distinct characteristics in symbols for vowel...Both the Todo Alphabet written with bambuu pen that is kept by Burintögs Khagai in County Zhaosu and the one written with soft brush that is kept by Namrjil Vjar have distinct characteristics in symbols for vowels,consonants,terminal letters and transcription letters.The two of them fundamentally adhered to the orthography established by Zaya Pandida and most likely were compiled in the transitional phase of the todo script.展开更多
Accurate atomic mass data hold significant application value in various research fields,in which Penning trap mass spectrometry is considered the most precise experimental method.A cryogenic detection system is a key ...Accurate atomic mass data hold significant application value in various research fields,in which Penning trap mass spectrometry is considered the most precise experimental method.A cryogenic detection system is a key component for reading out the image charge of charged particles in Penning traps using the Fourier transform ion cyclotron resonance technique.In this paper,we present the development and characteristics of this detection system,which includes a superconducting resonator and cryogenic low-noise amplifiers.The resonator consists of delicately woven thin NbTi wires configured into a multilayer helical coil,offering a quality factor of 98004 at around 1 MHz.Low-noise amplifiers are developed based on GaAs field effect transistors,exhibiting amplification factors greater than 27 dB with a power consumption of approximately 6 mW in the frequency range of 0.1 to 10 MHz.The lowest input voltage noise is 0.8√Hz at 1 MHz.The fabrication process,operation,and measurements are elucidated in detail.展开更多
【目的】Pen a 1是虾中的主要过敏原,已知其5个抗原表位在过敏反应中起着决定性的作用。用全蛋白抗体和表位特异性抗体,检测Pen a 1经体外模拟胃液(simulated gastric fluid,SGF)消化后其抗原表位免疫原性的变化情况,为研究人体消化对Pe...【目的】Pen a 1是虾中的主要过敏原,已知其5个抗原表位在过敏反应中起着决定性的作用。用全蛋白抗体和表位特异性抗体,检测Pen a 1经体外模拟胃液(simulated gastric fluid,SGF)消化后其抗原表位免疫原性的变化情况,为研究人体消化对Pen a 1的影响机理及脱敏食品的制备提供理论依据。【方法】以刀额新对虾(metapenaeus ensis)为原材料,提取纯化虾过敏原Pen a 1,采用Fmoc化学法合成Pen a 1抗原表位的5个多肽片段(No.1—No.5),分别与载体蛋白KLH和牛血清蛋白BSA偶联制备人工免疫原和包被原。用Pen a 1和制备的多肽免疫原免疫新西兰纯种白兔获得全白蛋抗体和特异性表位抗体。参照美国药典模拟人体胃液的条件消化处理Pen a 1,利用SDS-PAGE和Tricine-SDS-PAGE观察消化后Pen a 1分子量变化;通过免疫印迹(Western-blot)定性观察Pen a 1消化后生成的新蛋白片段与各抗体的结合情况;再经竞争抑制酶联免疫吸附试验(ci-ELISA)定量检测消化后的Pen a 1和表位多肽与各抗体结合能力,从而得出Pen a 1经SGF消化后各抗原表位免疫原性的变化程度。【结果】SDS-PAGE结果显示Pen a 1随SGF消化时间的延长逐渐被降解,在22 kD处生成一些较稳定的新蛋白片段,Tricine-SDS-PAGE结果表明在1.7—18 k D之间无新蛋白片段生成。Western-blot表明消化后分解生成的小分子蛋白与六种抗体发生不同程度结合,其中全蛋白抗体和No.4抗体几乎与生成的所有小分子蛋白结合,在22 k D左右处生成的稳定新蛋白片段均与No.3、4、5抗体有较强的结合,而与No.1、2抗体结合较弱甚至无结合,表明该蛋白携带No.3、4、5表位,而基本无No.1、2表位。ci-ELISA结果显示,Pen a 1经SGF消化后对全蛋白抗体和5个表位抗体的抑制率均显著下降,说明经过消化后Pena1及其抗原表位的免疫原性均明显降低,各抗原表位消化稳定性排序为No.4>No.2>No.1>No.3>No.5,其中No.2、1、3之间无显著性差异;ci-ELISA检测表位多肽经消化后其免疫原性也显著下降,消化稳定性排序为No.1>No.2>No.3>No.4>No.5,其中No.2、3、4之间无显著性差异。结合免疫印迹结果可知No.4抗原表位消化稳定性最高,但其表位多肽消化稳定性没有显著高于其它所有表位多肽,说明在消化过程中Pen a 1结构对该表位起到一定的保护作用,使其保持较高的消化稳定性;No.1、2、3次之,3个表位间无显著性差异;No.5消化稳定性最差,表明Pen a 1空间结构对其无明显保护作用。【结论】建立了一种用表位抗体检测过敏原经模拟消化后抗原表位消化稳定性的方法。Pen a 1经SGF消化后,免疫原性降低,分解生成部分较稳定的蛋白片段,但仍具有致敏性;5个抗原表位中No.4消化稳定最高,No.5最差。展开更多
文摘In the training studio of the School of Architecture and Engineering at Xizang Vocational Technical College,Lhasa,Gyatso,a senior artist specializing in Tibetan-style architectural decorative painting,bent over to demonstrate a student how to paint.His left hand steadied the painting board,while his right hand held a brush pen to present the Tibetan-style painting technique of"blurring"-dipping the brush lightly into the pigment,spreading it from dark to light on the paper.The eight-petaled lotus,a symbol of auspiciousness,gradually took shape,blossoming with layers of vitality.
文摘Conversation B Susie:I know.Pencils are also better for drawing.You can make lines lighter or darker.Sam:But you have to sharpen pencils or add lead to them.Susie:You do.But pencils last longer than pens.Pen ink can dry out,but pencil lead doesn’t.Sam:But words look neater when you write in pen.Susie:They do.So,which pen do you want?Sam:One with blue ink,thanks.
文摘一、阅读理解[核心素养·文化意识](2024·贵州铜仁一模) The brush pen, one of the “Four Treasures of the Study”, was invented thou-sands of years ago. However, the oldest pen was just a stick or a piece of bamboo. It was difficult for people to write well on thin silk.
文摘Both the Todo Alphabet written with bambuu pen that is kept by Burintögs Khagai in County Zhaosu and the one written with soft brush that is kept by Namrjil Vjar have distinct characteristics in symbols for vowels,consonants,terminal letters and transcription letters.The two of them fundamentally adhered to the orthography established by Zaya Pandida and most likely were compiled in the transitional phase of the todo script.
基金supported by the National Key R&D Program of China(No.2023YFA1606501)the National Natural Science Foundation of China(Nos.12204110 and 12474251)+2 种基金Shanghai Pujiang Program(No.22PJ1401100)Max-Planck Partner Group Projectthe Fudan University Yan Liyuan-EnSiKai Foundation(JX240003)。
文摘Accurate atomic mass data hold significant application value in various research fields,in which Penning trap mass spectrometry is considered the most precise experimental method.A cryogenic detection system is a key component for reading out the image charge of charged particles in Penning traps using the Fourier transform ion cyclotron resonance technique.In this paper,we present the development and characteristics of this detection system,which includes a superconducting resonator and cryogenic low-noise amplifiers.The resonator consists of delicately woven thin NbTi wires configured into a multilayer helical coil,offering a quality factor of 98004 at around 1 MHz.Low-noise amplifiers are developed based on GaAs field effect transistors,exhibiting amplification factors greater than 27 dB with a power consumption of approximately 6 mW in the frequency range of 0.1 to 10 MHz.The lowest input voltage noise is 0.8√Hz at 1 MHz.The fabrication process,operation,and measurements are elucidated in detail.
文摘【目的】Pen a 1是虾中的主要过敏原,已知其5个抗原表位在过敏反应中起着决定性的作用。用全蛋白抗体和表位特异性抗体,检测Pen a 1经体外模拟胃液(simulated gastric fluid,SGF)消化后其抗原表位免疫原性的变化情况,为研究人体消化对Pen a 1的影响机理及脱敏食品的制备提供理论依据。【方法】以刀额新对虾(metapenaeus ensis)为原材料,提取纯化虾过敏原Pen a 1,采用Fmoc化学法合成Pen a 1抗原表位的5个多肽片段(No.1—No.5),分别与载体蛋白KLH和牛血清蛋白BSA偶联制备人工免疫原和包被原。用Pen a 1和制备的多肽免疫原免疫新西兰纯种白兔获得全白蛋抗体和特异性表位抗体。参照美国药典模拟人体胃液的条件消化处理Pen a 1,利用SDS-PAGE和Tricine-SDS-PAGE观察消化后Pen a 1分子量变化;通过免疫印迹(Western-blot)定性观察Pen a 1消化后生成的新蛋白片段与各抗体的结合情况;再经竞争抑制酶联免疫吸附试验(ci-ELISA)定量检测消化后的Pen a 1和表位多肽与各抗体结合能力,从而得出Pen a 1经SGF消化后各抗原表位免疫原性的变化程度。【结果】SDS-PAGE结果显示Pen a 1随SGF消化时间的延长逐渐被降解,在22 kD处生成一些较稳定的新蛋白片段,Tricine-SDS-PAGE结果表明在1.7—18 k D之间无新蛋白片段生成。Western-blot表明消化后分解生成的小分子蛋白与六种抗体发生不同程度结合,其中全蛋白抗体和No.4抗体几乎与生成的所有小分子蛋白结合,在22 k D左右处生成的稳定新蛋白片段均与No.3、4、5抗体有较强的结合,而与No.1、2抗体结合较弱甚至无结合,表明该蛋白携带No.3、4、5表位,而基本无No.1、2表位。ci-ELISA结果显示,Pen a 1经SGF消化后对全蛋白抗体和5个表位抗体的抑制率均显著下降,说明经过消化后Pena1及其抗原表位的免疫原性均明显降低,各抗原表位消化稳定性排序为No.4>No.2>No.1>No.3>No.5,其中No.2、1、3之间无显著性差异;ci-ELISA检测表位多肽经消化后其免疫原性也显著下降,消化稳定性排序为No.1>No.2>No.3>No.4>No.5,其中No.2、3、4之间无显著性差异。结合免疫印迹结果可知No.4抗原表位消化稳定性最高,但其表位多肽消化稳定性没有显著高于其它所有表位多肽,说明在消化过程中Pen a 1结构对该表位起到一定的保护作用,使其保持较高的消化稳定性;No.1、2、3次之,3个表位间无显著性差异;No.5消化稳定性最差,表明Pen a 1空间结构对其无明显保护作用。【结论】建立了一种用表位抗体检测过敏原经模拟消化后抗原表位消化稳定性的方法。Pen a 1经SGF消化后,免疫原性降低,分解生成部分较稳定的蛋白片段,但仍具有致敏性;5个抗原表位中No.4消化稳定最高,No.5最差。
