Death following situations of intense emotional stress has been linked to the cardiac pathology described as stress cardiomyopathy, whose pathomechanism is still not clear. In this study, we sought to determine, via a...Death following situations of intense emotional stress has been linked to the cardiac pathology described as stress cardiomyopathy, whose pathomechanism is still not clear. In this study, we sought to determine, via an animal model, whether the transcriptional coactivator peroxisome proliferator-activated receptor γ coactivator-1alpha (PGC-1α) and the amino peptide neuropeptide Y (NPY) play a role in the pathogenesis of this cardiac entity. Male Sprague-Dawley rats in the experimental group were subjected to immobilization in a plexy glass box for 1 h, which was followed by low voltage elec-tric foot shock for about 1h at 10s intervals in a cage fitted with metallic rods. After 25 days the rats were sacrificed and sections of their hearts were processed. Hematoxylin-eosin staining of cardiac tissues revealed the characteristic cardiac lesions of stress cardiomyopathy such as contraction band necrosis, inflammatory cell infiltration and fibrosis. The semi-quantitative RT-PCR analysis for PGC-1α mRNA expression showed significant overexpression of PGC1-α in the stress-subjected rats (P<0.05). Fluorescence immunohistochemistry revealed a higher production of NPY in the stress-subjected rats as compared to the control rats (P=0.0027). Thus, we are led to conclude that following periods of intense stress, an increased expression of PGC1-α in the heart and an overflow of NPY may lead to stress car-diomyopathy and even death in susceptible victims. Moreover, these markers can be used to identify stress cardiomyopathy as the cause of sudden death in specific cases.展开更多
目的:探讨尾型同源盒转录因子2(caudal type homeobox transcription factor 2, CDX2)和Y 染色体上的性别决定区相关高迁移率族盒蛋白4(sex-dertermining region of Y chromosome related high mobility group box 4, SOX4)在胃癌组织...目的:探讨尾型同源盒转录因子2(caudal type homeobox transcription factor 2, CDX2)和Y 染色体上的性别决定区相关高迁移率族盒蛋白4(sex-dertermining region of Y chromosome related high mobility group box 4, SOX4)在胃癌组织中的表达及其临床意义。方法:采用Western Blot法检测CDX2 蛋白和SOX4 蛋白在50 例胃癌及其远癌胃组织中的表达,分析两者与胃癌临床病理特征之间的关系,Pearson 相关系数分析CDX2 蛋白、SOX4 蛋白在胃癌组织中表达的相关性;免疫荧光法检测蛋白细胞内定位。结果:免疫荧光通过镜下观察,CDX2 阳性信号在胃癌组织中主要位于细胞核,SOX4 阳性信号也见于细胞核。Western Blot 结果提示,CDX2 蛋白、SOX4 蛋白在胃癌组织中高表达,在远癌胃组织中低表达(P<0.05)。CDX2 蛋白表达与胃癌浸润深度、胃癌的TNM 分期、有无淋巴转移有关(均P<0. 05),与肿瘤分化程度、肿块大小、年龄和性别等无关(P>0.05)。SOX4 蛋白表达与胃癌有无淋巴转移和TMN分期有关(P<0. 05),与肿瘤分化程度、浸润深度、肿块大小、年龄和性别等无关(P>0.05)。Pearson 相关性分析提示在胃癌组织中CDX2 和SOX4 蛋白表达呈负相关(r=-0.476, P<0.05)。结论:CDX2 低表达和SOX4 高表达与胃癌病情进展有关,CDX2 和SOX4 在胃癌组织中表达呈负相关。联合检测CDX2和SOX4 的表达可作为反映胃癌临床病理学特点新的分子病理标志物。展开更多
基金supported by a grant from the National Natural Science Foundation of China(No.81172898)
文摘Death following situations of intense emotional stress has been linked to the cardiac pathology described as stress cardiomyopathy, whose pathomechanism is still not clear. In this study, we sought to determine, via an animal model, whether the transcriptional coactivator peroxisome proliferator-activated receptor γ coactivator-1alpha (PGC-1α) and the amino peptide neuropeptide Y (NPY) play a role in the pathogenesis of this cardiac entity. Male Sprague-Dawley rats in the experimental group were subjected to immobilization in a plexy glass box for 1 h, which was followed by low voltage elec-tric foot shock for about 1h at 10s intervals in a cage fitted with metallic rods. After 25 days the rats were sacrificed and sections of their hearts were processed. Hematoxylin-eosin staining of cardiac tissues revealed the characteristic cardiac lesions of stress cardiomyopathy such as contraction band necrosis, inflammatory cell infiltration and fibrosis. The semi-quantitative RT-PCR analysis for PGC-1α mRNA expression showed significant overexpression of PGC1-α in the stress-subjected rats (P<0.05). Fluorescence immunohistochemistry revealed a higher production of NPY in the stress-subjected rats as compared to the control rats (P=0.0027). Thus, we are led to conclude that following periods of intense stress, an increased expression of PGC1-α in the heart and an overflow of NPY may lead to stress car-diomyopathy and even death in susceptible victims. Moreover, these markers can be used to identify stress cardiomyopathy as the cause of sudden death in specific cases.
