Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible actio...Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.offi cinale flowers’flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34%of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-QTOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2%to 61.6%post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84%,43.85%,and 59.21%compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanolinduced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.展开更多
Environmental problem is a key problem for modern society. This paper analyzed indoor environment of the offi ce space in northeast China, explored the plants suitable for modular vertical greening in the offi ce spac...Environmental problem is a key problem for modern society. This paper analyzed indoor environment of the offi ce space in northeast China, explored the plants suitable for modular vertical greening in the offi ce space, and the key points for selection, and further analyzed the benefi ts of modular vertical greening for the indoor offi ce environment and the process fl ow. New technologies in modular vertical greening can be applied to improve the quality of indoor environment, and enhance the active atmosphere of indoor environment.展开更多
Enjoying a pleasant interior temperature in both summer and winter without the necessity for additional heating or cooling—a level of comfort already offered by private homes built with an eye to energy effi ciency.W...Enjoying a pleasant interior temperature in both summer and winter without the necessity for additional heating or cooling—a level of comfort already offered by private homes built with an eye to energy effi ciency.What has become known as the passive house standard is now increasingly demanded in tenders and competitions revolving around offi ce buildings.Below,Dr Friedrich Sick,professor at the HTW Berlin,and Stefan Schade,a graduate in Environmental Engineering/Renewable Energies who explored this particular fi eld in his thesis[1],examine the conditions necessary for the implementation of this standard.展开更多
基金supported by National Key Research and Development Program for Intergovernmental International Science and Technology Innovation Cooperation(2024YFE0109500)National Natural Science Foundation of China(32072149).
文摘Gastric ulcer is a widespread disease caused by various etiologies.Dendrobium offi cinale flowers exert several health benefits owing to their rich flavonoid content.In this study,protective effects and possible action mechanisms of D.offi cinale flowers’flavonoid enriched extract(DOFF)were assessed against gastric ulcer.The result of sodium nitrite-aluminum nitrate colorimetry showed that 52.34%of the total extractive was flavonoid,and ultra-high performance liquid chromatography time of flight mass spectrometer(UPLC-QTOF/MS)revealed the presence of 28 components in DOFF of which 14 belonged to flavonoids.In addition,in vivo assay revealed DOFF potential in reducing the formation of ethanol-induced gastric mucosal lesions,with drop-in ulcer index from 64.33±8.76 to 32.00±4.47.Similar results were revealed in human gastric mucosal epithelia cells,with cells viability to increase from 27.2%to 61.6%post DOFF administration.To analyzed the protect effect of DOFF,we used Western blotting and immunofluorometric assay to revealed the expression levels of key proteins in cell pathways.The results showed that DOFF(320μg/mL)could increase the level of oxidation marker protein(HO-1),apoptosis regulatory protein(Bcl-2)and autophagy marker(LC3β)by 50.84%,43.85%,and 59.21%compared with ethanol-treated group respectively.Further analyzed of the mitochondrial activity and apoptosis pathway,we found that DOFF appeared to mitigate against ethanolinduced gastric mucosal injury via AMPK/mTOR/ULK1 and PI3K/AKT autophagy signaling pathways.
文摘Environmental problem is a key problem for modern society. This paper analyzed indoor environment of the offi ce space in northeast China, explored the plants suitable for modular vertical greening in the offi ce space, and the key points for selection, and further analyzed the benefi ts of modular vertical greening for the indoor offi ce environment and the process fl ow. New technologies in modular vertical greening can be applied to improve the quality of indoor environment, and enhance the active atmosphere of indoor environment.
文摘Enjoying a pleasant interior temperature in both summer and winter without the necessity for additional heating or cooling—a level of comfort already offered by private homes built with an eye to energy effi ciency.What has become known as the passive house standard is now increasingly demanded in tenders and competitions revolving around offi ce buildings.Below,Dr Friedrich Sick,professor at the HTW Berlin,and Stefan Schade,a graduate in Environmental Engineering/Renewable Energies who explored this particular fi eld in his thesis[1],examine the conditions necessary for the implementation of this standard.
