Objective To quantitatively evaluate the metabolic changes of benign prostatic hyperplasia(BPH) and prostate cancer in the transitional zone using magnetic resonance spectroscopic imaging(MRSI),and to analyze the char...Objective To quantitatively evaluate the metabolic changes of benign prostatic hyperplasia(BPH) and prostate cancer in the transitional zone using magnetic resonance spectroscopic imaging(MRSI),and to analyze the characteristics and differences of the spectra in this zonal area.Methods Eighteen patients with prostate cancer in the transitional zone underwent magnetic resonance imaging(MRI)/MRSI examinations.The(Choline+Creatine)/Citrate(CC/Ci) ratio and the Choline/Creatine(Cho/Cr) ratio were evaluated in each voxel with cancer or BPH confirmed by pathological results.Discriminant analysis was used to determine the power of the two ratios in differentiation between cancer and BPH.Results The CC/Ci ratio and Cho/Cr ratio for cancer voxels were significantly higher than those in the voxels with BPH in the transitional zone(CC/Ci:2.36±1.31 vs.0.85±0.29,P<0.01;Cho/Cr:4.14±1.79 vs.1.26±0.45,P<0.01).As for the discriminant function with the CC/Ci ratio and the Cho/Cr ratio,the specificity,sensitivity,and accuracy were 98.6%,85.7%,92.9% respectively for the differentiation between cancer and BPH.Conclusions The prostate cancer is characterized by higher CC/Ci ratio and Cho/Cr ratio compared to BPH in the transitional zone.Both CC/Ci ratio and Cho/Cr ratio have high specificity,sensitivity,and accuracy in their discriminative power between cancer and BPH in this zonal area.展开更多
Photoacoustic imaging using a closed photoacoustic cell and an open photoacoustic cell with gas- microphone detection scheme was described. R/G/B LED light sources were used for the closed photoacoustic (PA) cell conf...Photoacoustic imaging using a closed photoacoustic cell and an open photoacoustic cell with gas- microphone detection scheme was described. R/G/B LED light sources were used for the closed photoacoustic (PA) cell configuration. The colored specimen enclosed in a PA cell was imaged with R/G/B color light sources, and an image restored from the inverted PA images was compared with the original image. For open cell configuration, an open PA cell using a spheroidal acoustic resonator was applied to measure the amount of large-sized colored specimens. A calibration curve for a food red dye was obtained that apparently showed the ability of the present scheme to measure as a spectroscopic measurement tool.展开更多
AIM To assess the relationship using multimodality imaging between intermediary citrate/choline metabolism as seen on proton magnetic resonance spectroscopic imaging(1H-MRSI) and glycolysis as observed on ^(18)F-fluor...AIM To assess the relationship using multimodality imaging between intermediary citrate/choline metabolism as seen on proton magnetic resonance spectroscopic imaging(1H-MRSI) and glycolysis as observed on ^(18)F-fluorodeoxyglucose positron emission tomography/computed tomography(^(18)F-FDG-PET/CT) in prostate cancer(PCa) patients. METHODS The study included 22 patients with local PCa who were referred for endorectal magnetic resonance imaging/1HMRSI(April 2002 to July 2007) and ^(18)F-FDG-PET/CT and then underwent prostatectomy as primary or salvage treatment. Whole-mount step-section pathology was used as the standard of reference. We assessed the relationships between PET parameters [standardized uptake value(SUVmax and SUVmean)] and MRSI parameters [choline + creatine/citrate(CC/Cmax and CC/Cmean) and total number of suspicious voxels] using spearman's rank correlation, and the relationships of PET and 1H-MRSI index lesion parameters to surgical Gleason score.RESULTS Abnormal intermediary metabolism on 1H-MRSI was present in 21/22 patients, while abnormal glycolysis on ^(18)F-FDG-PET/CT was detected in only 3/22 patients. Specifically, index tumor localization rates were 0.95(95%CI: 0.77-1.00) for 1H-MRSI and 0.14(95%CI: 0.03-0.35) for ^(18)F-FDG-PET/CT. Spearman rank correlations indicated little relationship(ρ =-0.36-0.28) between 1H-MRSI parameters and ^(18)F-FDG-PET/CT parameters. Both the total number of suspicious voxels(ρ = 0.55, P = 0.0099) and the SUVmax(ρ = 0.46, P = 0.0366) correlated weakly with the Gleason score. No significant relationship was found between the CC/Cmax, CC/Cmean or SUVmean and the Gleason score(P = 0.15-0.79). CONCLUSION The concentration of intermediary metabolites detected by 1H MRSI and glycolytic flux measured ^(18)F-FDG PET show little correlation. Furthermore, only few tumors were FDG avid on PET, possibly because increased glycolysis represents a late and rather ominous event in the progression of PCa.展开更多
Hyperspectral photothermal mid-infrared spectroscopic imaging(HP-MIRSI)is an emerging technology with promising applications in cervical cancer diagnosis and quantitative,label-free histopathology.This study pioneers ...Hyperspectral photothermal mid-infrared spectroscopic imaging(HP-MIRSI)is an emerging technology with promising applications in cervical cancer diagnosis and quantitative,label-free histopathology.This study pioneers the application of HP-MIRSI to the evaluation of clinical cervical cancer tissues,achieving excellent tissue type segmentation accuracy of over 95%.This achievement stems from an integrated approach of optimized data acquisition,computational data reconstruction,and the application of machine learning algorithms.The results are statistically robust,drawing from tissue samples of 98 cervical cancer patients and incorporating over 40 million data points.Traditional cervical cancer diagnosis methods entail biopsy,staining,and visual evaluation by a pathologist.This process is qualitative,subject to variations in staining and subjective interpretations,and requires extensive tissue processing,making it costly and time-consuming.In contrast,our proposed alternative can produce images comparable to those from histological analyses without the need for staining or complex sample preparation.This label-free,quantitative method utilizes biochemical data from HPMIRSI and employs machine-learning algorithms for the rapid and precise segmentation of cervical tissue subtypes.This approach can potentially transform histopathological analysis by offering a more accurate and label-free alternative to conventional diagnostic processes.展开更多
Spectral imaging—a suite of techniques combining image acquisition with extremely high color resolution—plays an ever-increasing role in many fields,such as biomedicine,agriculture,geology,archeology,and environment...Spectral imaging—a suite of techniques combining image acquisition with extremely high color resolution—plays an ever-increasing role in many fields,such as biomedicine,agriculture,geology,archeology,and environmental control.^(1-3)The capability of visualizing,in real time,a tissue or terrain with spatially resolved chemical sensitivity can literally mean the difference between life and death.To appreciate the significance,consider how in vivo spectroscopic sensing of malignant tissue empowers the surgeon to minimize collateral damage during tumor removal while keeping the risk of cancer recurrence low.展开更多
The 21 cm radiation of neutral hydrogen provides crucial information for studying the early universe and its evolution.To advance this research,countries have made significant investments in constructing large lowfreq...The 21 cm radiation of neutral hydrogen provides crucial information for studying the early universe and its evolution.To advance this research,countries have made significant investments in constructing large lowfrequency radio telescope arrays,such as the Low Frequency Array and the Square Kilometre Array Phase 1 Low Frequency.These instruments are pivotal for radio astronomy research.However,challenges such as ionospheric plasma interference,ambient radio noise,and instrument-related effects have become increasingly prominent,posing major obstacles in cosmology research.To address these issues,this paper proposes an efficient signal processing method that combines wavelet transform and mathematical morphology.The method involves the following steps:Background Subtraction:Background interference in radio observation signals is eliminated.Wavelet Transform:The signal,after removing background noise,undergoes a two-dimensional discrete wavelet transform.Threshold processing is then applied to the wavelet coefficients to effectively remove interference components.Wavelet Inversion:The processed signal is reconstructed using wavelet inversion.Mathematical Morphology:The reconstructed signal is further optimized using mathematical morphology to refine the results.Experimental verification was conducted using solar observation data from the Xinjiang Observatory and the Yunnan Observatory.The results demonstrate that this method successfully removes interference signals while preserving useful signals,thus improving the accuracy of radio astronomy observations and reducing the impact of radio frequency interference.展开更多
文摘Objective To quantitatively evaluate the metabolic changes of benign prostatic hyperplasia(BPH) and prostate cancer in the transitional zone using magnetic resonance spectroscopic imaging(MRSI),and to analyze the characteristics and differences of the spectra in this zonal area.Methods Eighteen patients with prostate cancer in the transitional zone underwent magnetic resonance imaging(MRI)/MRSI examinations.The(Choline+Creatine)/Citrate(CC/Ci) ratio and the Choline/Creatine(Cho/Cr) ratio were evaluated in each voxel with cancer or BPH confirmed by pathological results.Discriminant analysis was used to determine the power of the two ratios in differentiation between cancer and BPH.Results The CC/Ci ratio and Cho/Cr ratio for cancer voxels were significantly higher than those in the voxels with BPH in the transitional zone(CC/Ci:2.36±1.31 vs.0.85±0.29,P<0.01;Cho/Cr:4.14±1.79 vs.1.26±0.45,P<0.01).As for the discriminant function with the CC/Ci ratio and the Cho/Cr ratio,the specificity,sensitivity,and accuracy were 98.6%,85.7%,92.9% respectively for the differentiation between cancer and BPH.Conclusions The prostate cancer is characterized by higher CC/Ci ratio and Cho/Cr ratio compared to BPH in the transitional zone.Both CC/Ci ratio and Cho/Cr ratio have high specificity,sensitivity,and accuracy in their discriminative power between cancer and BPH in this zonal area.
文摘Photoacoustic imaging using a closed photoacoustic cell and an open photoacoustic cell with gas- microphone detection scheme was described. R/G/B LED light sources were used for the closed photoacoustic (PA) cell configuration. The colored specimen enclosed in a PA cell was imaged with R/G/B color light sources, and an image restored from the inverted PA images was compared with the original image. For open cell configuration, an open PA cell using a spheroidal acoustic resonator was applied to measure the amount of large-sized colored specimens. A calibration curve for a food red dye was obtained that apparently showed the ability of the present scheme to measure as a spectroscopic measurement tool.
基金Supported by National Institutes of Health grant,No.#R01CA76423in part through the NIH/NCI Cancer Center Support grant,No.P30 CA008748
文摘AIM To assess the relationship using multimodality imaging between intermediary citrate/choline metabolism as seen on proton magnetic resonance spectroscopic imaging(1H-MRSI) and glycolysis as observed on ^(18)F-fluorodeoxyglucose positron emission tomography/computed tomography(^(18)F-FDG-PET/CT) in prostate cancer(PCa) patients. METHODS The study included 22 patients with local PCa who were referred for endorectal magnetic resonance imaging/1HMRSI(April 2002 to July 2007) and ^(18)F-FDG-PET/CT and then underwent prostatectomy as primary or salvage treatment. Whole-mount step-section pathology was used as the standard of reference. We assessed the relationships between PET parameters [standardized uptake value(SUVmax and SUVmean)] and MRSI parameters [choline + creatine/citrate(CC/Cmax and CC/Cmean) and total number of suspicious voxels] using spearman's rank correlation, and the relationships of PET and 1H-MRSI index lesion parameters to surgical Gleason score.RESULTS Abnormal intermediary metabolism on 1H-MRSI was present in 21/22 patients, while abnormal glycolysis on ^(18)F-FDG-PET/CT was detected in only 3/22 patients. Specifically, index tumor localization rates were 0.95(95%CI: 0.77-1.00) for 1H-MRSI and 0.14(95%CI: 0.03-0.35) for ^(18)F-FDG-PET/CT. Spearman rank correlations indicated little relationship(ρ =-0.36-0.28) between 1H-MRSI parameters and ^(18)F-FDG-PET/CT parameters. Both the total number of suspicious voxels(ρ = 0.55, P = 0.0099) and the SUVmax(ρ = 0.46, P = 0.0366) correlated weakly with the Gleason score. No significant relationship was found between the CC/Cmax, CC/Cmean or SUVmean and the Gleason score(P = 0.15-0.79). CONCLUSION The concentration of intermediary metabolites detected by 1H MRSI and glycolytic flux measured ^(18)F-FDG PET show little correlation. Furthermore, only few tumors were FDG avid on PET, possibly because increased glycolysis represents a late and rather ominous event in the progression of PCa.
基金supported in part by the Cancer Prevention and Research Institute of Texas(CPRIT)no.RR170075(RR)National Institutes of Health(NIH)grant no.R01DK135870 and no.R01HL173597(RR)+2 种基金NLM Training Program in Biomedical Informatics and Data Science no.T15LM007093(RR and SB)National Institutes of Health no.R01HL146745(DM)the National Science Foundation CAREER Award no.1943455(DM).
文摘Hyperspectral photothermal mid-infrared spectroscopic imaging(HP-MIRSI)is an emerging technology with promising applications in cervical cancer diagnosis and quantitative,label-free histopathology.This study pioneers the application of HP-MIRSI to the evaluation of clinical cervical cancer tissues,achieving excellent tissue type segmentation accuracy of over 95%.This achievement stems from an integrated approach of optimized data acquisition,computational data reconstruction,and the application of machine learning algorithms.The results are statistically robust,drawing from tissue samples of 98 cervical cancer patients and incorporating over 40 million data points.Traditional cervical cancer diagnosis methods entail biopsy,staining,and visual evaluation by a pathologist.This process is qualitative,subject to variations in staining and subjective interpretations,and requires extensive tissue processing,making it costly and time-consuming.In contrast,our proposed alternative can produce images comparable to those from histological analyses without the need for staining or complex sample preparation.This label-free,quantitative method utilizes biochemical data from HPMIRSI and employs machine-learning algorithms for the rapid and precise segmentation of cervical tissue subtypes.This approach can potentially transform histopathological analysis by offering a more accurate and label-free alternative to conventional diagnostic processes.
文摘Spectral imaging—a suite of techniques combining image acquisition with extremely high color resolution—plays an ever-increasing role in many fields,such as biomedicine,agriculture,geology,archeology,and environmental control.^(1-3)The capability of visualizing,in real time,a tissue or terrain with spatially resolved chemical sensitivity can literally mean the difference between life and death.To appreciate the significance,consider how in vivo spectroscopic sensing of malignant tissue empowers the surgeon to minimize collateral damage during tumor removal while keeping the risk of cancer recurrence low.
基金funded by the National Key Research and Development Program’s intergovernmental International Science and Technology Innovation Cooperation project,titled Remote Sensing and Radio Astronomy Observation of Space Weather in Low and Middle Latitudes(project number:2022YFE0140000)Supported by International Partnership Program of Chinese Academy of Sciences,grant No.114A11KYSB20200001。
文摘The 21 cm radiation of neutral hydrogen provides crucial information for studying the early universe and its evolution.To advance this research,countries have made significant investments in constructing large lowfrequency radio telescope arrays,such as the Low Frequency Array and the Square Kilometre Array Phase 1 Low Frequency.These instruments are pivotal for radio astronomy research.However,challenges such as ionospheric plasma interference,ambient radio noise,and instrument-related effects have become increasingly prominent,posing major obstacles in cosmology research.To address these issues,this paper proposes an efficient signal processing method that combines wavelet transform and mathematical morphology.The method involves the following steps:Background Subtraction:Background interference in radio observation signals is eliminated.Wavelet Transform:The signal,after removing background noise,undergoes a two-dimensional discrete wavelet transform.Threshold processing is then applied to the wavelet coefficients to effectively remove interference components.Wavelet Inversion:The processed signal is reconstructed using wavelet inversion.Mathematical Morphology:The reconstructed signal is further optimized using mathematical morphology to refine the results.Experimental verification was conducted using solar observation data from the Xinjiang Observatory and the Yunnan Observatory.The results demonstrate that this method successfully removes interference signals while preserving useful signals,thus improving the accuracy of radio astronomy observations and reducing the impact of radio frequency interference.
