Herein,porous poly(lactic-co-glycolic acid)(PLGA)microspheres were prepared to load icariin andmiR-23b for the treatment of metastatic lung cancer.The microspheres exhibited desirable aerodynamic diameter,high drug lo...Herein,porous poly(lactic-co-glycolic acid)(PLGA)microspheres were prepared to load icariin andmiR-23b for the treatment of metastatic lung cancer.The microspheres exhibited desirable aerodynamic diameter,high drug loading and encapsulation efficiency,as well as a favorable drug release profile,which was beneficial for the deposition and exposure of drugs in the lung tissues.The release solution from microspheres exhibited a favorable anti-proliferative effect by inducting cell apoptosis and arresting the cell cycle at G1 phase,and meanwhile inhibited the migration and invasion of cancer cells.More importantly,the microspheres could be effectively inhaled and accumulated in the lung tissues to trigger the in situ apoptosis of tumor cells and suppress metastasis,using mice bearing melanoma-metastatic lung cancer as a model.Furthermore,inhalation of themicrospheres showed favorable biocompatibility,barely causing tissue damage.Overall,porous PLGA microspheres provide a promising platform for the inhalable co-delivery of drugs and genes to obtain ideal therapeutic efficacy in lung cancer and other pulmonary diseases.展开更多
[目的]探讨miR-23b-3p靶向调节TFRC对子宫内膜癌Ishikawa细胞转移活性的影响。[方法]设Ishikawa子宫内膜癌细胞组、miRNA-NC组、miR-23b-3p inhibitor组、miR-23b-3p mimics组,培养72h后,测定细胞活力、凋亡及细胞侵袭迁移水平,RT-PCR...[目的]探讨miR-23b-3p靶向调节TFRC对子宫内膜癌Ishikawa细胞转移活性的影响。[方法]设Ishikawa子宫内膜癌细胞组、miRNA-NC组、miR-23b-3p inhibitor组、miR-23b-3p mimics组,培养72h后,测定细胞活力、凋亡及细胞侵袭迁移水平,RT-PCR法、蛋白印迹法测定细胞miR-23b-3p、TFRC水平。[结果]与Ishikawa子宫内膜癌细胞组、miRNA-NC组比较,miR-23b-3p inhibitor组OD值(0.84±0.05 vs 0.83±0.06 vs 0.93±0.07,P<0.05)、存活率(88.85%±2.33%vs 86.34%±2.74%vs 95.27%±2.25%,P<0.05)、穿膜数、迁移距离(18.36±5.25 vs 19.33±6.30 vs 65.63±12.38,P<0.05)升高,凋亡率降低(6.11%±1.25%vs 6.83%±1.18%vs 1.13%±0.24%,P<0.05);miR-23b-3p mimics组的Ishikawa细胞的OD值、存活率、穿膜数、迁移距离降低,凋亡率升高(P<0.05);与miR-23b-3p inhibitor组比较,miR-23b-3p mimics组的Ishikawa细胞的OD值、存活率、穿膜数、迁移距离降低,凋亡率升高(P<0.05)。与Ishikawa子宫内膜癌细胞组、miRNA-NC组比较,miR-23b-3p inhibitor组的Ishikawa细胞的miR-23b-3p表达水平降低,TFRC mRNA水平和蛋白表达水平升高(0.95±0.15 vs 1.78±0.11,P<0.05),miR-23b-3p mimics组的Ishikawa细胞的miR-23b-3p表达水平升高,TFRC mRNA和蛋白表达水平降低(0.95±0.15 vs 0.34±0.17,P<0.05);与miR-23b-3p inhibitor组比较,miR-23b-3p mimics组的Ishikawa细胞的miR-23b-3p表达水平升高,TFRC mRNA和蛋白表达水平降低(1.78±0.11 vs 0.34±0.17,P<0.05)。[结论]miR-23b-3p靶向抑制TFRC表达进而抑制子宫内膜癌Ishikawa细胞的转移。展开更多
目的研究miR-23a和miR-23b在非小细胞肺癌中的表达特征,并探讨其临床意义。方法收集157例非小细胞肺癌手术切除标本及50例癌旁组织标本,采用Real time PCR方法检测miR-23a和miR-23b在非小细胞肺癌及其癌旁组织中的表达,分析二者表达的...目的研究miR-23a和miR-23b在非小细胞肺癌中的表达特征,并探讨其临床意义。方法收集157例非小细胞肺癌手术切除标本及50例癌旁组织标本,采用Real time PCR方法检测miR-23a和miR-23b在非小细胞肺癌及其癌旁组织中的表达,分析二者表达的相关性,并探讨其表达与临床病理特征及其预后的关系。结果 miR-23a和miR-23b在非小细胞肺癌组织中的表达水平均高于癌旁肺组织,二者在非小细胞肺癌组织中表达呈正相关(r=0.351,P<0.001)。miR-23a和miR-23b联合高表达与淋巴结有无转移(P<0.001)、远处转移(P=0.001)及临床分期(P=0.002)相关,而与年龄、性别、组织类型及组织分化程度无显著相关性(P>0.05)。KaplanMeier分析显示miR-23a和miR-23b联合高表达组患者生存期显著低于单独高表达组或联合低表达组(P=0.009),Cox风险比例模型分析显示miR-23a和miR-23b联合高表达为非小细胞肺癌患者的危险因素。结论 miR-23a和miR-23b在非小细胞肺癌中异常高表达可能是潜在的肺癌预后分子标志物。展开更多
基金the National Natural Science Foundation of China(32271319 and 32071267)the Science and Technology Department of Jilin Province(YDZJ202301ZYTS537 and 20240402035GH)+1 种基金the Development and Reform Commission of Jilin Province(2023C015)the“Medicine+X”cross-innovation team of Bethune Medical Department of Jilin University“Leading the Charge with Open Competition”construction project(2022JBGS04).
文摘Herein,porous poly(lactic-co-glycolic acid)(PLGA)microspheres were prepared to load icariin andmiR-23b for the treatment of metastatic lung cancer.The microspheres exhibited desirable aerodynamic diameter,high drug loading and encapsulation efficiency,as well as a favorable drug release profile,which was beneficial for the deposition and exposure of drugs in the lung tissues.The release solution from microspheres exhibited a favorable anti-proliferative effect by inducting cell apoptosis and arresting the cell cycle at G1 phase,and meanwhile inhibited the migration and invasion of cancer cells.More importantly,the microspheres could be effectively inhaled and accumulated in the lung tissues to trigger the in situ apoptosis of tumor cells and suppress metastasis,using mice bearing melanoma-metastatic lung cancer as a model.Furthermore,inhalation of themicrospheres showed favorable biocompatibility,barely causing tissue damage.Overall,porous PLGA microspheres provide a promising platform for the inhalable co-delivery of drugs and genes to obtain ideal therapeutic efficacy in lung cancer and other pulmonary diseases.
文摘[目的]探讨miR-23b-3p靶向调节TFRC对子宫内膜癌Ishikawa细胞转移活性的影响。[方法]设Ishikawa子宫内膜癌细胞组、miRNA-NC组、miR-23b-3p inhibitor组、miR-23b-3p mimics组,培养72h后,测定细胞活力、凋亡及细胞侵袭迁移水平,RT-PCR法、蛋白印迹法测定细胞miR-23b-3p、TFRC水平。[结果]与Ishikawa子宫内膜癌细胞组、miRNA-NC组比较,miR-23b-3p inhibitor组OD值(0.84±0.05 vs 0.83±0.06 vs 0.93±0.07,P<0.05)、存活率(88.85%±2.33%vs 86.34%±2.74%vs 95.27%±2.25%,P<0.05)、穿膜数、迁移距离(18.36±5.25 vs 19.33±6.30 vs 65.63±12.38,P<0.05)升高,凋亡率降低(6.11%±1.25%vs 6.83%±1.18%vs 1.13%±0.24%,P<0.05);miR-23b-3p mimics组的Ishikawa细胞的OD值、存活率、穿膜数、迁移距离降低,凋亡率升高(P<0.05);与miR-23b-3p inhibitor组比较,miR-23b-3p mimics组的Ishikawa细胞的OD值、存活率、穿膜数、迁移距离降低,凋亡率升高(P<0.05)。与Ishikawa子宫内膜癌细胞组、miRNA-NC组比较,miR-23b-3p inhibitor组的Ishikawa细胞的miR-23b-3p表达水平降低,TFRC mRNA水平和蛋白表达水平升高(0.95±0.15 vs 1.78±0.11,P<0.05),miR-23b-3p mimics组的Ishikawa细胞的miR-23b-3p表达水平升高,TFRC mRNA和蛋白表达水平降低(0.95±0.15 vs 0.34±0.17,P<0.05);与miR-23b-3p inhibitor组比较,miR-23b-3p mimics组的Ishikawa细胞的miR-23b-3p表达水平升高,TFRC mRNA和蛋白表达水平降低(1.78±0.11 vs 0.34±0.17,P<0.05)。[结论]miR-23b-3p靶向抑制TFRC表达进而抑制子宫内膜癌Ishikawa细胞的转移。
文摘目的研究miR-23a和miR-23b在非小细胞肺癌中的表达特征,并探讨其临床意义。方法收集157例非小细胞肺癌手术切除标本及50例癌旁组织标本,采用Real time PCR方法检测miR-23a和miR-23b在非小细胞肺癌及其癌旁组织中的表达,分析二者表达的相关性,并探讨其表达与临床病理特征及其预后的关系。结果 miR-23a和miR-23b在非小细胞肺癌组织中的表达水平均高于癌旁肺组织,二者在非小细胞肺癌组织中表达呈正相关(r=0.351,P<0.001)。miR-23a和miR-23b联合高表达与淋巴结有无转移(P<0.001)、远处转移(P=0.001)及临床分期(P=0.002)相关,而与年龄、性别、组织类型及组织分化程度无显著相关性(P>0.05)。KaplanMeier分析显示miR-23a和miR-23b联合高表达组患者生存期显著低于单独高表达组或联合低表达组(P=0.009),Cox风险比例模型分析显示miR-23a和miR-23b联合高表达为非小细胞肺癌患者的危险因素。结论 miR-23a和miR-23b在非小细胞肺癌中异常高表达可能是潜在的肺癌预后分子标志物。
