摘要
目的探讨miR-23a对LPS诱导小鼠原代腹腔巨噬细胞促炎性细胞因子表达的影响。方法 Real-time PCR定量分析LPS刺激不同时间的腹腔巨噬细胞中miR-23a的表达以及瞬时转染miR-23a mimics和inhibitors的腹腔巨噬细胞中IL-1β、IL-6和TNF-α等促炎性细胞因子的表达;ELISA检测mRNA变化明显的细胞培养上清的IL-6的含量。结果原代腹腔巨噬细胞在LPS刺激后,miR-23a的表达明显下调;在转染miR-23a mimics后,IL-6的表达和分泌明显升高(P<0.05),但对IL-1β和TNF-α的表达没有明显影响。而转染miR-23a inhibitors后,则IL-6的表达水平受到抑制。结论 LPS刺激原代巨噬细胞可抑制miR-23a的表达;miR-23a可促进炎性细胞因子IL-6的表达,对IL-1β和TNF-α无显著影响。
Objective To investigate the effect of miR-23a on the pro-inflammatory cytokine expression in murine peritoneal macrophage stimulated with LPS. Methods Real-time PCR was used to analyze the expression of miR 23a in mouse macrophage treated with LPS and the expression of pro-inflammatory cytokines including IL-1β, IL-6 and TNF-a in mouse peritoneal macrophage transfected with miR-23a mimics or inhibitors; ELISA was used to measure the IL-6 secretion. Results After LPS stimulation, the expression of miR-23a in the mouse peritoneal macrophage was remarkably decreased. While the cells transfected with miR-23a mimics, the expression and secre- tion of IL-6 was significantly increased （P 〈 0. 05 ）, without notable influence on IL-1β and TNF-αexpression. Af- ter transfected with miR-23a inhibitors, the expression of IL-6 was decreased. Conclusions LPS stimulation can suppress the expression of miR-23a, miR-23a can promote the expression of pro-inflammatory cytokine IL-6, but have no notable influence on IL-1β and TNF-α.
出处
《基础医学与临床》
CSCD
北大核心
2013年第6期666-670,共5页
Basic and Clinical Medicine
基金
国家自然科学基金(30972684
810013151)
