[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the...[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the obtained transformants were detected by PCR.[Result] A single square electric pulse could be applied to cell sample in 0.2 cm ET cuvettes by using a Bio-Rad Gene Pulser set at 150 V,25 ms at room temperature,but the transformation efficiency was very low.[Conclusion] This research may improve the transformation efficiency of T.maritima MS8.展开更多
Soil contamination by toxic trace metal elements,like barium(Ba),may stimulate various undesirable changes in the metabolic activity of plants.The plant responses are fast and with,direct or indirect,generation of rea...Soil contamination by toxic trace metal elements,like barium(Ba),may stimulate various undesirable changes in the metabolic activity of plants.The plant responses are fast and with,direct or indirect,generation of reactive oxygen species(ROS).To cope with the stress imposed by the ROS production,plants developed a dual cellular system composed of enzymatic and non-enzymatic players that convert ROS,and their by-products,into stable nontoxic molecules.To assess the Ba stress response of two Brassicaceae species(Brassica juncea,a glycophyte,and Cakile maritime,a halophyte),plants were exposure to different Ba concentrations(0,100,200,300 and 500µM).The plants response was evaluated through their morphology and development,the determination of plant leaves antioxidant enzymatic activities and by the production of plants secondary metabolites.Results indicated that the two Brassicaceae species have the ability to survive in an environment containing Ba(even at 500µM).The biomass production of C.maritima was slightly affected whereas an increase in biomass B.juncea was noticed.The stress imposed by Ba activated the antioxidant defense system in the two species,noticed by the changes in the leaves activity of catalase(CAT),ascorbate peroxidase(APX)and guaicol peroxidase(GPX),and of the secondary metabolites,through the production of total phenols and flavonoids.The enzymatic response was not similar within the two plant species:CAT and APX seem to have a more important role against the oxidative stress in C.maritima while in B.juncea is GPX.Overall,total phenols and flavonoids production was more significant in the plants aerial part than in the roots,of the both species.Although the two Brassicaceae species response was different,in both plants catalytic and non-catalytic transformation of ROS occurs,and both were able to overcome the Ba toxicity and prevent the cell damage.展开更多
Objective:To investigate the in vitro antioxidant and antimicrobial activities along with phytochemical screening of organic and aqueous extracts of leaf and stem of Suaeda maritima (Dumort).a mangrove associate from ...Objective:To investigate the in vitro antioxidant and antimicrobial activities along with phytochemical screening of organic and aqueous extracts of leaf and stem of Suaeda maritima (Dumort).a mangrove associate from Bhitarkanika of Odisha.India.Methods:Antioxidant activity of the crode extracts was evaluated in terms of total antioxidant capacity,total phenol content,ascorbic acid content,DPPH radical scavenging,metal chelating,nitric oxide scavenging,and reducing power etc.The antimicrobial activity of the plant was determined by agar well diffusion method along with MIC and BBC carried out by microdilulion techniques against 10 gram positive and gram negative human pathogenic bacteria.The qualitative and qualitative phytochemical screening were carried out by standard biochemical assays.Results: Out of the seven antioxidant bioassavs,both the leaf and stem extracts were found to posses strong antioxidant properties of 70%to 92%for phenol,total antioxidant capacity,DPPH free radical scavenging aclivitv and fairly good ascorbic acid content,metal chelating(1.33%-22.55%). reducing power(0.01-0.12) and nitric oxide scavenging(0.84%-66.99%) activities.Out of the four extracts evaluated for antimicrobial activity,two leaf extracts such as acetone and ethanol showed promising activity against four pathogenic bacteria and one stem methanol extracts against one pathogenic bacteria when compared with amoxcycillin as standard.The MIC and MBC values of the antimicrobial extracts ranged between 2.5 to 5.0 mg/mL.Screening of phytochemicals showed presence of carbohydrates,protein,tannins,alkaloids and flavonoids in comparatively higher amount than other phytochemicals tested.Conclusions:The present study reveals the presence of potential antioxidants and antimicrobial properties in the plant extract which could be exploited for pharmaceutical application.展开更多
Lobularia maritima(L.)Desv.is an ornamental plant cultivated across the world.It belongs to the family Brassicaceae and can tolerate dry,poor and contaminated habitats.Here,we present a chromosome-scale,high-quality g...Lobularia maritima(L.)Desv.is an ornamental plant cultivated across the world.It belongs to the family Brassicaceae and can tolerate dry,poor and contaminated habitats.Here,we present a chromosome-scale,high-quality genome assembly of L.maritima based on integrated approaches combining Illumina short reads and Hi–C chromosome conformation data.The genome was assembled into 12 pseudochromosomes with a 197.70 Mb length,and it includes 25,813 protein-coding genes.Approximately 41.94%of the genome consists of repetitive sequences,with abundant long terminal repeat transposable elements.Comparative genomic analysis confirmed that L.maritima underwent a species-specific whole-genome duplication(WGD)event~22.99 million years ago.We identified~1900 species-specific genes,25 expanded gene families,and 50 positively selected genes in L.maritima.Functional annotations of these genes indicated that they are mainly related to stress tolerance.These results provide new insights into the stress tolerance of L.maritima,and this genomic resource will be valuable for further genetic improvement of this important ornamental plant.展开更多
Gene encoding endo-β-1,4-glucanase(TM1525)is derived from Thermotoga maritima(T.maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase.This enzyme has the same high tem...Gene encoding endo-β-1,4-glucanase(TM1525)is derived from Thermotoga maritima(T.maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase.This enzyme has the same high temperature resistance as thermophilic bacteria,which is an ideal property for industrial applications.By molecular biological means,TM1525 was cloned into pHT43 vector and introduced into Bacillus subtilis(B.subtilis)WB800N by electroporation.The results showed that the WB800N expression system was successfully constructed,and extracellular expression of the recombinant gene was achieved.Cellulose hydrolyzed activity of the protein was exhibited.展开更多
Bacteria form biofilms as an adaptive mechanism in response to environmental changes. Streptococcus mutans is the biofilm-forming bacterium that is primarily associated with dental caries. The expression of important ...Bacteria form biofilms as an adaptive mechanism in response to environmental changes. Streptococcus mutans is the biofilm-forming bacterium that is primarily associated with dental caries. The expression of important genes by bacteria in biofilms is different from that of planktonic cells. Lectins are proteins that bind specifically to carbohydrates and may have important biological activities on bacterial cells, acting as antibacterial and anti-biofilm agents. ConM (Canavalia maritima lectin) is a protein that is able to inhibit the planktonic growth and biofilm formation of S. mutans. In this context, this study aimed to evaluate the effects of ConM and concanavalin A (ConA) on the expression of genes related to virulence and biofilm formation in S. mutans. The results showed that ConM significantly reduced the expression of genes encoding enzymes related to adhesion, formation and regulation of biofilms. On the contrary, ConA did not alter the expression of the genes studied. Because the two lectins have a high degree of similarity, the differences in the actions of ConM and ConA may be explained by the small structural differences in the carbohydrate recognition domain of the lectins.展开更多
目的基于文献研究归纳新外来中药海葱的中药药性,应用智能感官进一步验证并探讨其五味药性,为新外来中药的药性研究提供思路。方法通过中国知识资源总库(CNKI)、中文科技期刊数据库(VIP)、中国学术期刊数据库(Wanfang Data)、PubMed、We...目的基于文献研究归纳新外来中药海葱的中药药性,应用智能感官进一步验证并探讨其五味药性,为新外来中药的药性研究提供思路。方法通过中国知识资源总库(CNKI)、中文科技期刊数据库(VIP)、中国学术期刊数据库(Wanfang Data)、PubMed、Web of Science检索海葱相关研究文献,结合中医理论归纳海葱的中药药性。应用PEN3型电子鼻和SA402B型电子舌采集海葱的智能感官信息,利用主成分分析法辨识海葱的五味药性。结果通过文献研究归纳得到海葱具有抗氧化、抗肿瘤、强心利尿、止咳平喘等药理作用,结合理论探讨可知,海葱性微温,味苦、辛、甘,有小毒,归肺、脾、胃、心经。基于智能感官的海葱五味药性辨识结果为苦、微辛、微甘、咸、非酸,与文献研究结果基本一致。结论通过文献研究结合智能感官可以较好地归纳和辨识海葱的中药药性,本研究可为客观、充分地探析新外来中药的药性提供参考。展开更多
基金Supported by Sub-Project of"973"(2004CB719600)National Natural Science Foundation Projects(30170511)~~
文摘[Objective] The aim was to study on the electrotransformation of Thermotoga maritima MS8.[Method] Square waves,exponential waves and high voltage shock were used for the electrotransformation of T.maritima MS8,and the obtained transformants were detected by PCR.[Result] A single square electric pulse could be applied to cell sample in 0.2 cm ET cuvettes by using a Bio-Rad Gene Pulser set at 150 V,25 ms at room temperature,but the transformation efficiency was very low.[Conclusion] This research may improve the transformation efficiency of T.maritima MS8.
基金This work has been supported by LISBOA-01-0145-FERDER-031863 project,cofunded by FEDER through POR Lisboa 2020(Programa Operacional de Lisboa)from Portugal 2020 and Fundação para a Ciência e a Tecnologia(PTDC/CTA-AMB/31863/2017).The authors also gratefully acknowledge the support of CERENA(strategic project FCT-UIDB/04028/2020)and MARE(strategic project FCT-UIDB/04292/2020).
文摘Soil contamination by toxic trace metal elements,like barium(Ba),may stimulate various undesirable changes in the metabolic activity of plants.The plant responses are fast and with,direct or indirect,generation of reactive oxygen species(ROS).To cope with the stress imposed by the ROS production,plants developed a dual cellular system composed of enzymatic and non-enzymatic players that convert ROS,and their by-products,into stable nontoxic molecules.To assess the Ba stress response of two Brassicaceae species(Brassica juncea,a glycophyte,and Cakile maritime,a halophyte),plants were exposure to different Ba concentrations(0,100,200,300 and 500µM).The plants response was evaluated through their morphology and development,the determination of plant leaves antioxidant enzymatic activities and by the production of plants secondary metabolites.Results indicated that the two Brassicaceae species have the ability to survive in an environment containing Ba(even at 500µM).The biomass production of C.maritima was slightly affected whereas an increase in biomass B.juncea was noticed.The stress imposed by Ba activated the antioxidant defense system in the two species,noticed by the changes in the leaves activity of catalase(CAT),ascorbate peroxidase(APX)and guaicol peroxidase(GPX),and of the secondary metabolites,through the production of total phenols and flavonoids.The enzymatic response was not similar within the two plant species:CAT and APX seem to have a more important role against the oxidative stress in C.maritima while in B.juncea is GPX.Overall,total phenols and flavonoids production was more significant in the plants aerial part than in the roots,of the both species.Although the two Brassicaceae species response was different,in both plants catalytic and non-catalytic transformation of ROS occurs,and both were able to overcome the Ba toxicity and prevent the cell damage.
基金field staffs and forest oivision,Rajnagar for their kind cooperation and support
文摘Objective:To investigate the in vitro antioxidant and antimicrobial activities along with phytochemical screening of organic and aqueous extracts of leaf and stem of Suaeda maritima (Dumort).a mangrove associate from Bhitarkanika of Odisha.India.Methods:Antioxidant activity of the crode extracts was evaluated in terms of total antioxidant capacity,total phenol content,ascorbic acid content,DPPH radical scavenging,metal chelating,nitric oxide scavenging,and reducing power etc.The antimicrobial activity of the plant was determined by agar well diffusion method along with MIC and BBC carried out by microdilulion techniques against 10 gram positive and gram negative human pathogenic bacteria.The qualitative and qualitative phytochemical screening were carried out by standard biochemical assays.Results: Out of the seven antioxidant bioassavs,both the leaf and stem extracts were found to posses strong antioxidant properties of 70%to 92%for phenol,total antioxidant capacity,DPPH free radical scavenging aclivitv and fairly good ascorbic acid content,metal chelating(1.33%-22.55%). reducing power(0.01-0.12) and nitric oxide scavenging(0.84%-66.99%) activities.Out of the four extracts evaluated for antimicrobial activity,two leaf extracts such as acetone and ethanol showed promising activity against four pathogenic bacteria and one stem methanol extracts against one pathogenic bacteria when compared with amoxcycillin as standard.The MIC and MBC values of the antimicrobial extracts ranged between 2.5 to 5.0 mg/mL.Screening of phytochemicals showed presence of carbohydrates,protein,tannins,alkaloids and flavonoids in comparatively higher amount than other phytochemicals tested.Conclusions:The present study reveals the presence of potential antioxidants and antimicrobial properties in the plant extract which could be exploited for pharmaceutical application.
基金supported by the National Key Research and Development Program of China(2017YFC0505203)National Natural Science Foundation of China(31590821)+2 种基金Fundamental Research Funds for the Central Universities(2018CDDY-S02-SCU and SCU2019D013)National High-Level Talents Special Support Plan(10 Thousand of People Plan)985 and 211 Projects of Sichuan University.
文摘Lobularia maritima(L.)Desv.is an ornamental plant cultivated across the world.It belongs to the family Brassicaceae and can tolerate dry,poor and contaminated habitats.Here,we present a chromosome-scale,high-quality genome assembly of L.maritima based on integrated approaches combining Illumina short reads and Hi–C chromosome conformation data.The genome was assembled into 12 pseudochromosomes with a 197.70 Mb length,and it includes 25,813 protein-coding genes.Approximately 41.94%of the genome consists of repetitive sequences,with abundant long terminal repeat transposable elements.Comparative genomic analysis confirmed that L.maritima underwent a species-specific whole-genome duplication(WGD)event~22.99 million years ago.We identified~1900 species-specific genes,25 expanded gene families,and 50 positively selected genes in L.maritima.Functional annotations of these genes indicated that they are mainly related to stress tolerance.These results provide new insights into the stress tolerance of L.maritima,and this genomic resource will be valuable for further genetic improvement of this important ornamental plant.
基金National Natural Science Foundation of China(Regional Fund)(No.51863020)
文摘Gene encoding endo-β-1,4-glucanase(TM1525)is derived from Thermotoga maritima(T.maritima),which has an open reading frame of 825 bp and encodes a 274 amino acid endo-β-1,4-glucanase.This enzyme has the same high temperature resistance as thermophilic bacteria,which is an ideal property for industrial applications.By molecular biological means,TM1525 was cloned into pHT43 vector and introduced into Bacillus subtilis(B.subtilis)WB800N by electroporation.The results showed that the WB800N expression system was successfully constructed,and extracellular expression of the recombinant gene was achieved.Cellulose hydrolyzed activity of the protein was exhibited.
文摘Bacteria form biofilms as an adaptive mechanism in response to environmental changes. Streptococcus mutans is the biofilm-forming bacterium that is primarily associated with dental caries. The expression of important genes by bacteria in biofilms is different from that of planktonic cells. Lectins are proteins that bind specifically to carbohydrates and may have important biological activities on bacterial cells, acting as antibacterial and anti-biofilm agents. ConM (Canavalia maritima lectin) is a protein that is able to inhibit the planktonic growth and biofilm formation of S. mutans. In this context, this study aimed to evaluate the effects of ConM and concanavalin A (ConA) on the expression of genes related to virulence and biofilm formation in S. mutans. The results showed that ConM significantly reduced the expression of genes encoding enzymes related to adhesion, formation and regulation of biofilms. On the contrary, ConA did not alter the expression of the genes studied. Because the two lectins have a high degree of similarity, the differences in the actions of ConM and ConA may be explained by the small structural differences in the carbohydrate recognition domain of the lectins.
文摘目的基于文献研究归纳新外来中药海葱的中药药性,应用智能感官进一步验证并探讨其五味药性,为新外来中药的药性研究提供思路。方法通过中国知识资源总库(CNKI)、中文科技期刊数据库(VIP)、中国学术期刊数据库(Wanfang Data)、PubMed、Web of Science检索海葱相关研究文献,结合中医理论归纳海葱的中药药性。应用PEN3型电子鼻和SA402B型电子舌采集海葱的智能感官信息,利用主成分分析法辨识海葱的五味药性。结果通过文献研究归纳得到海葱具有抗氧化、抗肿瘤、强心利尿、止咳平喘等药理作用,结合理论探讨可知,海葱性微温,味苦、辛、甘,有小毒,归肺、脾、胃、心经。基于智能感官的海葱五味药性辨识结果为苦、微辛、微甘、咸、非酸,与文献研究结果基本一致。结论通过文献研究结合智能感官可以较好地归纳和辨识海葱的中药药性,本研究可为客观、充分地探析新外来中药的药性提供参考。
