[Objective] This study aimed to carry out a preliminary analysis of genetic diversity of 47 JUNCAO germplasms. [Methods] Twenty-eight iPBS （Intel Primer Binding Site Amplification） primers were firstly used for PCR ...[Objective] This study aimed to carry out a preliminary analysis of genetic diversity of 47 JUNCAO germplasms. [Methods] Twenty-eight iPBS （Intel Primer Binding Site Amplification） primers were firstly used for PCR screening on a subset of four germplasms, of which 11 gave good amplification patterns and were then used for analyzing the DNA of 47 JUNCAO germplasms. [Result] A total of 208 polymorphic DNA fragments were scored among the 47 JUNCAO germplasms from the electrophoresis patterns of the 11 selected iPBS primers. By using the NTSYSpc 2.1 software combined with UPGMA clustering analysis method, the simple matching （SM） coefficient of similarity was calculated among all accessions and ranged from 0.58 to 0.99. The 47 JUNCAO germplasms were clustered into 10 categories at a genetic similarity coefficient of 0.67. All the 47 accessions were distinguished from each other. [Conclusion] Our results showed that iPBS markers could be effectively used for genetic diversity analysis of JUNCAO germplasms. This study provides a preliminary theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.展开更多
SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to ampli...SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to amplify four representative germplasms to screen the best primer pairs that could produce poly-morphic DNA fragments. As a result, a total of 284 polymorphic DNA fragments were scored among the 48 JUNCAO germplasms from the electrophoresis patterns generated with 18 selected SRAP primer pairs. By using NTSYS-pc 2.1 software combined with UPGMA cluster analysis, the genetic similarity （SM） coefficient be- tween the 48 accessions was calculated and ranged from 0.58 to 0.98. All the 48 accessions were distinguished from each other. The 48 JUNCAO germplasms were classified into four categories at a genetic similarity coefficient of 0.645. The results showed that the SRAP markers could be effectively used for analysis of genetic di- versify of JUNCAO germplasms. Additionally, the results also showed that there ex- ist abundant JUNCAO germptasm in the genus Arundo in China. This study provides a new technique for studying the genetic diversify of JUNCAO germplasms. The selected combinations of SRAP primers can be used for genetic analyses on a larger number of JUNCAO germplasms in the future. Moreover, the results provide theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.展开更多
基金Supported by R&D Program of China National Engineering Research Center o JUNCAO Technology(JCGG14010)~~
文摘[Objective] This study aimed to carry out a preliminary analysis of genetic diversity of 47 JUNCAO germplasms. [Methods] Twenty-eight iPBS （Intel Primer Binding Site Amplification） primers were firstly used for PCR screening on a subset of four germplasms, of which 11 gave good amplification patterns and were then used for analyzing the DNA of 47 JUNCAO germplasms. [Result] A total of 208 polymorphic DNA fragments were scored among the 47 JUNCAO germplasms from the electrophoresis patterns of the 11 selected iPBS primers. By using the NTSYSpc 2.1 software combined with UPGMA clustering analysis method, the simple matching （SM） coefficient of similarity was calculated among all accessions and ranged from 0.58 to 0.99. The 47 JUNCAO germplasms were clustered into 10 categories at a genetic similarity coefficient of 0.67. All the 47 accessions were distinguished from each other. [Conclusion] Our results showed that iPBS markers could be effectively used for genetic diversity analysis of JUNCAO germplasms. This study provides a preliminary theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.
文摘SRAP （Sequence-related amplified polymorphism）, a PCR-based molecular marker system was used to evaluate the genetic diversity of 48 JUCAO germplasms in this study. At first, 256 SRAP primer pairs were used to amplify four representative germplasms to screen the best primer pairs that could produce poly-morphic DNA fragments. As a result, a total of 284 polymorphic DNA fragments were scored among the 48 JUNCAO germplasms from the electrophoresis patterns generated with 18 selected SRAP primer pairs. By using NTSYS-pc 2.1 software combined with UPGMA cluster analysis, the genetic similarity （SM） coefficient be- tween the 48 accessions was calculated and ranged from 0.58 to 0.98. All the 48 accessions were distinguished from each other. The 48 JUNCAO germplasms were classified into four categories at a genetic similarity coefficient of 0.645. The results showed that the SRAP markers could be effectively used for analysis of genetic di- versify of JUNCAO germplasms. Additionally, the results also showed that there ex- ist abundant JUNCAO germptasm in the genus Arundo in China. This study provides a new technique for studying the genetic diversify of JUNCAO germplasms. The selected combinations of SRAP primers can be used for genetic analyses on a larger number of JUNCAO germplasms in the future. Moreover, the results provide theoretical guidance and technical support for the efficient management and utilization of JUNCAO germplasm resources.
