摘要
菌草灵芝是用菌草代替木屑栽培出的灵芝,有多种生物活性,已被证实有较强的体外免疫作用。环磷酰胺作为烷化剂类抗肿瘤药物,也是典型的免疫抑制剂,会造成机体免疫损伤。本研究以环磷酰胺诱导小鼠免疫低下模型,灌胃100 mg/(kg·d)的菌草灵芝醇提物(JGEH),研究其对小鼠免疫损伤的改善情况。结果:JGEH可显著(P<0.05)提高小鼠体质量(6.32%)、脏器指数(胸腺38.50%、脾脏71.32%、肝脏1.27%和肾脏指数11.48%)、碳廓清能力(41.50%),迟发型变态反应程度(59.50%)。此外,JGEH还能显著(P<0.05)提高小鼠血清中IgG(27.73%)、IgA(26.51%)、IgM(23.41%)和IL-10(41.98%)等免疫球蛋白和抗炎因子含量,降低TNF-α(17.40%)、IFN-γ(17.49%)和IL-6(42.33%)等炎症细胞因子含量,显著提高(P<0.05)全血中白细胞(41.23%)、红细胞(18.91%)、血红蛋白(42.32%)、血小板含量(40.29%)及淋巴细胞百分比(159.94%),为菌草灵芝醇提物的发展提供研究思路。
Juncao Ganoderma lucidum,which is cultivated with Juncao instead of wood chips,has a wide range of bioactivities and has been shown to have a strong in vitro immune effect.Cyclophosphamide,as an alkylating antitumor agent,is also a typical immunosuppressant that causes immune damage to the body.In the present study,cyclophosphamide-induced immunocompromise model in mice was investigated by gavage of 100 mg/(kg·d)Juncao Ganoderma lucidum alcohol to study the improvement of immune injury in mice.The results showed that JGEH significantly(P<0.05)increased body weight(6.32%),organ indices(thymus 38.50%,spleen 71.32%,liver 1.27%,and kidney indices 11.48%),carbon contouring capacity(41.50%),and the degree of delayed metamorphosis(59.50%)in mice.In addition,JGEH significantly(P<0.05)increased immunoglobulin and anti-inflammatory factors,such as IgG(27.73%),IgA(26.51%),IgM(23.41%),and IL-10(41.98%),and decreased inflammatory cytokines,such as TNF-α(17.40%),IFN-γ(17.49%),and IL-6(42.33%),in serum,and significantly(P<0.05)increased leukocytes(41.23%),erythrocytes(18.91%),hemoglobin(42.32%),platelets(40.29%),and percentage of lymphocytes(159.94%)in whole blood of mice.Research ideas for the development of alcoholic extracts of the mycorrhizal Juncao Ganoderma lucidum.
作者
张凤丽
黄文琪
赵立娜
ZHANG Fengli;HUANG Wenqi;ZHAO Lina(National Engineering Research Center of Juncao Technology,Fujian Agriculture and Forestry University,Fuzhou 350002;College of Life Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002)
出处
《中国食品学报》
北大核心
2025年第1期151-159,共9页
Journal of Chinese Institute Of Food Science and Technology
基金
福建省科学技术厅重大专项(2021NZ029009)
福建省科学技术厅对外合作项目(2021I0008)。
关键词
菌草灵芝
环磷酰胺
小鼠
免疫活性
Juncao Ganoderma lucidum
cyclophosphamide
mice
immunological activity
