Background and AimsInfections are frequent and lethal complications of acute-on-chronic liver failure(ACLF).Reliable biomarkers to distinguish fungal from bacterial infections remain limited.Given the central role of ...Background and AimsInfections are frequent and lethal complications of acute-on-chronic liver failure(ACLF).Reliable biomarkers to distinguish fungal from bacterial infections remain limited.Given the central role of immune dysfunction in ACLF,we aimed to evaluate the diagnostic value of serum cytokines in differentiating invasive pulmonary aspergillosis(IPA)from bacterial pneumonia(BP)in HBV-associated ACLF.MethodsThis retrospective case-control study enrolled ACLF patients admitted to the Tongji Hospital,between 2018 and 2022.Patients were categorized into IPA,BP,and non-infection groups.The BP and non-infection groups were propensity score-matched to the IPA cases.Serum cytokines levels(IL-1β,sIL-2R,IL-6,IL-8,IL-10,TNF-α)and clinical data were collected,with the diagnostic performance of these cytokines as biomarkers assessed via ROC curves.ResultsA total of 32 IPA,96 BP,and 96 non-infection patients were enrolled,with balanced baseline characteristics.Compared with the non-infection group,the IPA group had higher sIL-2R(1,606.00 vs.1,211.50 U/mL,P=0.019)and IL-6(69.03 vs.15.98 pg/mL,P<0.001)levels,but lower IL-8 levels(62.20 vs.132.00 pg/mL,P=0.025).The BP group showed elevated sIL-2R(1,792.00 U/mL),IL-6(49.42 pg/mL),IL-10(13.40 pg/mL)levels compared to the non-infection group(all P<0.001).Also,IL-8 was lower in the IPA group than in the BP group(62.20 vs.176.00 pg/mL,P<0.001)and its assessment could best distinguish IPA from BP(AUC=0.743,cut-off=76.60 pg/mL;sensitivity=66.7%,specificity=82.1%).ConclusionsSerum IL-8 exhibited superior diagnostic value for IPA in patients with HBV-ACLF and could effectively discriminate Aspergillus infections from bacterial infections.展开更多
AIM： To find out if a functional promoter polymorphism in the IL-8 gene along with cagA status and polymorphisms in vacA gene influence the type of diseases in Iranian patients infected by Hpylori. METHODS： IL-8 -25...AIM： To find out if a functional promoter polymorphism in the IL-8 gene along with cagA status and polymorphisms in vacA gene influence the type of diseases in Iranian patients infected by Hpylori. METHODS： IL-8 -251 A/T polymorphism was genotyped by oligonucleotide allele specific PCR （ASO-PCR） in a sample of 233 patients with Hpylori infection undergoing upper gastrointestinal endoscopy. The presence of cagA gene and polymorphisms in vacA gene was also determined by PCR. Association of these genetic polymorphisms with the development of gastritis, peptic ulcers as well as gastric cancer was tested. RESULTS： When the patients with different clinical manifestations were compared according to the presence of cagA gene or various vacA genotypes, only the vacA genotypes were significantly different among gastritis, peptic ulcer and gastric cancer patients （χ^2= 17.8; P=0.001）. Furthermore, there was a significant difference in the frequency of IL-8 -251 A/T genotypes between patients with gastric cancer and benign diseases （χ^2=10.47;P=0.005） CONCLUSION： The IL-8 -251 A/T polymorphism and the polymorphisms in H pylor/ vacA gene are involved in limiting the infection outcome to gastritis and peptic ulcer or in favoring cancer onset in Iranian patients.展开更多
基金supported by the National Key Research and Development Program of China(grant number 2023YFC2308405).
文摘Background and AimsInfections are frequent and lethal complications of acute-on-chronic liver failure(ACLF).Reliable biomarkers to distinguish fungal from bacterial infections remain limited.Given the central role of immune dysfunction in ACLF,we aimed to evaluate the diagnostic value of serum cytokines in differentiating invasive pulmonary aspergillosis(IPA)from bacterial pneumonia(BP)in HBV-associated ACLF.MethodsThis retrospective case-control study enrolled ACLF patients admitted to the Tongji Hospital,between 2018 and 2022.Patients were categorized into IPA,BP,and non-infection groups.The BP and non-infection groups were propensity score-matched to the IPA cases.Serum cytokines levels(IL-1β,sIL-2R,IL-6,IL-8,IL-10,TNF-α)and clinical data were collected,with the diagnostic performance of these cytokines as biomarkers assessed via ROC curves.ResultsA total of 32 IPA,96 BP,and 96 non-infection patients were enrolled,with balanced baseline characteristics.Compared with the non-infection group,the IPA group had higher sIL-2R(1,606.00 vs.1,211.50 U/mL,P=0.019)and IL-6(69.03 vs.15.98 pg/mL,P<0.001)levels,but lower IL-8 levels(62.20 vs.132.00 pg/mL,P=0.025).The BP group showed elevated sIL-2R(1,792.00 U/mL),IL-6(49.42 pg/mL),IL-10(13.40 pg/mL)levels compared to the non-infection group(all P<0.001).Also,IL-8 was lower in the IPA group than in the BP group(62.20 vs.176.00 pg/mL,P<0.001)and its assessment could best distinguish IPA from BP(AUC=0.743,cut-off=76.60 pg/mL;sensitivity=66.7%,specificity=82.1%).ConclusionsSerum IL-8 exhibited superior diagnostic value for IPA in patients with HBV-ACLF and could effectively discriminate Aspergillus infections from bacterial infections.
基金Supported by a grant numbered 82-1774 from Shiraz University of Medical Sciences
文摘AIM： To find out if a functional promoter polymorphism in the IL-8 gene along with cagA status and polymorphisms in vacA gene influence the type of diseases in Iranian patients infected by Hpylori. METHODS： IL-8 -251 A/T polymorphism was genotyped by oligonucleotide allele specific PCR （ASO-PCR） in a sample of 233 patients with Hpylori infection undergoing upper gastrointestinal endoscopy. The presence of cagA gene and polymorphisms in vacA gene was also determined by PCR. Association of these genetic polymorphisms with the development of gastritis, peptic ulcers as well as gastric cancer was tested. RESULTS： When the patients with different clinical manifestations were compared according to the presence of cagA gene or various vacA genotypes, only the vacA genotypes were significantly different among gastritis, peptic ulcer and gastric cancer patients （χ^2= 17.8; P=0.001）. Furthermore, there was a significant difference in the frequency of IL-8 -251 A/T genotypes between patients with gastric cancer and benign diseases （χ^2=10.47;P=0.005） CONCLUSION： The IL-8 -251 A/T polymorphism and the polymorphisms in H pylor/ vacA gene are involved in limiting the infection outcome to gastritis and peptic ulcer or in favoring cancer onset in Iranian patients.
