摘要
AIM： To find out if a functional promoter polymorphism in the IL-8 gene along with cagA status and polymorphisms in vacA gene influence the type of diseases in Iranian patients infected by Hpylori. METHODS： IL-8 -251 A/T polymorphism was genotyped by oligonucleotide allele specific PCR （ASO-PCR） in a sample of 233 patients with Hpylori infection undergoing upper gastrointestinal endoscopy. The presence of cagA gene and polymorphisms in vacA gene was also determined by PCR. Association of these genetic polymorphisms with the development of gastritis, peptic ulcers as well as gastric cancer was tested. RESULTS： When the patients with different clinical manifestations were compared according to the presence of cagA gene or various vacA genotypes, only the vacA genotypes were significantly different among gastritis, peptic ulcer and gastric cancer patients （χ^2= 17.8; P=0.001）. Furthermore, there was a significant difference in the frequency of IL-8 -251 A/T genotypes between patients with gastric cancer and benign diseases （χ^2=10.47;P=0.005） CONCLUSION： The IL-8 -251 A/T polymorphism and the polymorphisms in H pylor/ vacA gene are involved in limiting the infection outcome to gastritis and peptic ulcer or in favoring cancer onset in Iranian patients.
瞄准：发现是否在与 cagA 地位一起的 IL-8 基因的功能的倡导者多型性和在基因影响的休假的多型性在伊朗的病人的疾病的类型由 H pylori 感染了。方法：IL-8 -251 A/T 多型性在有经历上面的胃肠的内视镜检查法的 H pylori 感染的 233 个病人的一件样品是由 oligonucleotide 等位基因 specific PCR (ASO-PCR ) 的 genotyped。在基因也是的休假的 cagA 基因和多型性的存在由 PCR 决定了。有胃炎，消化性溃疡以及胃的癌症的发展的这些基因多型性的协会被测试。结果：当有不同临床的表明的病人根据 cagA 基因或各种各样的休假 A 遗传型的存在被比较时，仅仅休假 A 遗传型在胃炎，消化性溃疡和胃的癌症病人之中是显著地不同的(chi 2 = 17.8；P = 0.001 ) 。而且，在在有胃的癌症和良性的疾病的病人之间的 IL-8 -251 A/T 遗传型的频率有有效差量(chi 2 = 10.47；P = 0.005 ) 。结论：在 H pylori 休假的 IL-8 -251 A/T 多型性和多型性基因涉及限制感染结果到胃炎和消化性溃疡或在在伊朗的病人赞成癌症发作。
基金
Supported by a grant numbered 82-1774 from Shiraz University of Medical Sciences
