摘要
目的 :观察肾衰宁 (SSN)灌肠液对体外培养人肾小球系膜细胞增殖及产生白介素 8(IL 8)的影响 ,从细胞生物学水平探讨 SSN防治慢性肾功能衰竭 (CRF)的作用机制。方法 :采用 4甲基偶氮唑盐 (MTT)比色法和双抗夹心酶联免疫吸附法 (EL ISA) ,观察 SSN大鼠血清对体外培养人肾小球系膜细胞增殖和 IL 8生成的影响。结果 :内毒素 (L PS)可明显刺激人肾小球系膜细胞增殖和产生 IL 8,SSN大鼠血清对 L PS刺激后人肾小球系膜细胞增殖和产生 IL 8有抑制作用 ,其抑制程度与药物浓度呈量效关系。结论 :SSN抑制系膜细胞增殖和自分泌 IL 8是预防肾小球硬化发生发展的重要作用机制之一。
Objective:To observed the influences of traditional Chinese drugs——Shenshuaining(SSN,肾衰宁) on proliferation of human glomerular mesangial cells(huMsC)and production of interleukin8(IL8) was producted by huMsC cultured in vitro,and to approach the mechanism of SSN on prevention and treatment of chronic renal failure(CRF) in level of cell biology.Methods:The effects of SSN rat serum on proliferation of huMsC and the production of IL8 by huMsC cultured in vitro were observed.MTT colourmetry and enzymelinked immunosorbent assay(ELISA) were used.Results:Proliferation of huMsC and the production of IL8 were significantly stimulated by lipopolysaccharide(LPS) in huMsC cultured in vitro,whereas significantly inhibited by SSN rat serum and inhibition rate was relative to dosage.Conclusions:It is one of the important mechanism of preventing the occurence and development of glomerulosclerosis that proliferation of huMsC and the production of IL8 which produced by MsC were inhibited by SSN.
出处
《中国中西医结合急救杂志》
CAS
2000年第4期197-199,共3页
Chinese Journal of Integrated Traditional and Western Medicine in Intensive and Critical Care
基金
中国人民武装警察部队医学院科研基金!资助项目( WY990 5 )
关键词
肾功能衰竭
肾衰宁
人肾小球系膜细胞
中药
IL-8
chronic renal failure
Shenshuaining
human glomerular mesangial cell
interleukin8
mechanism
traditional Chinese medicine
