Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying supe...Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying superflow formation remain insufficiently understood.In this work,we reveal these mechanisms by studying the time evolution of the transferred total angular momentum and the quantized current throughout the phase imprinting process,achieved through numerically solving the time-dependent Schrodinger and Gross-Pitaevskii equations.We demonstrate that the Bose gas dynamically acquires angular momentum through the density depletion induced by the phase imprinting potential,whereas quantized currents emerge from azimuthal phase slips accompanied by complete density depletions.Regarding the impact of system parameters,such as interactions,we find that interactions hinder superflow formation,as the azimuthal density distribution becomes less susceptible to the phase imprinting potential.Our findings offer microscopic insights into the dynamic development of superflow during the phase imprinting process and provide valuable guidance for ongoing experimental efforts.展开更多
Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly ...Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly imprinted photoelectrochemical(PEC)sensor based on the Zn O/C photoanode and the Fe-doped CuBi_(2)O_(4)(CBFO)photocathode is developed for the sensitive detection of TC.The photocathodic current can be amplified by the efficient electron transfer caused by the Fermi energy level gap between the photoanode and photocathode.Furthermore,molecularly imprinted polymers(MIPs)at photocathode can selectivity identify the TC templates and thus improve the specificity.Under the optimal conditions,the sensor has a linear range of 10^(-2)-1.0×10^(5) nmol/L,and a limit of detection(LOD)of 0.007 nmol/L(S/N=3).More crucially,the milk sample detection is carried out using the as-prepared sensor,and the outcome is satisfactory.The research gives us a novel sensing platform for quick and accurate antibiotic(like TC)in environment and food monitoring.展开更多
Background The primary differentially methylated regions(DMRs) which are maternally hypermethylated serve as imprinting control regions(ICRs) that drive monoallelic gene expression, and these ICRs have been investigat...Background The primary differentially methylated regions(DMRs) which are maternally hypermethylated serve as imprinting control regions(ICRs) that drive monoallelic gene expression, and these ICRs have been investigated due to their implications in mammalian development. Although a subset of genes has been identified as imprinted, in-depth comparative approach needs to be developed for identification of species-specific imprinted genes. Here, we examined DNA methylation status and allelic expression at the KBTBD6 locus across species and tissues and explored potential mechanisms of imprinting.Results Using whole-genome bisulfite sequencing and RNA-sequencing on parthenogenetic and normal porcine embryos, we identified a maternally hypermethylated DMR between the embryos at the KBTBD6 promoter Cp G island and paternal monoallelic expression of KBTBD6. Also, in analyzed domesticated mammals but not in humans, non-human primates and mice, the KBTBD6 promoter Cp G islands were methylated in oocytes and/or allelically methyl-ated in tissues, and monoallelic KBTBD6 expression was observed, indicating livestock-specific imprinting. Further analysis revealed that these Cp G islands were embedded within transcripts in porcine and bovine oocytes which coexisted with an active transcription mark and DNA methylation, implying the presence of transcription-dependent imprinting.Conclusions In this study, our comparative approach revealed an imprinted expression of the KBTBD6 gene in domesticated mammals, but not in humans, non-human primates, and mice which implicates species-specific evolution of genomic imprinting.展开更多
Copper is a microelement with important physiological functions in the body.However,the excess copper ion(Cu^(2+))may cause severe health problems,such as hair cell apoptosis and the resultant hearing loss.Therefore,t...Copper is a microelement with important physiological functions in the body.However,the excess copper ion(Cu^(2+))may cause severe health problems,such as hair cell apoptosis and the resultant hearing loss.Therefore,the assay of Cu^(2+)is important.We integrate ionic imprinting technology(IIT)and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads(IIHBs)as a low-cost and high-specificity platform for Cu^(2+)detection.The IIHBs have a macroporous microstructure,uniform size,vivid structural color,and magnetic responsiveness.When incubated in solution,IIHBs recognize Cu^(2+)and exhibit a reflective peak change,thereby achieving label-free detection.In addition,benefiting from the IIT,the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100μmol·L^(-1).These features indicated that the developed IIHBs are promising candidates for Cu^(2+)detection,particularly for the prevention of hearing loss.展开更多
As a new concept having emerged in last few years,the“deep eutectic solvents”(DESs)effect integrated into the imprinting technology inevitably exposes design limitations of stimuli-responsive molecularly imprinted p...As a new concept having emerged in last few years,the“deep eutectic solvents”(DESs)effect integrated into the imprinting technology inevitably exposes design limitations of stimuli-responsive molecularly imprinted polymers(MIPs),as well as inadequate analysis of the adsorption performance of MIPs.Herein,a simple yet defined N-isopropylacrylamide/(3-acrylamidopropyl)trimethylammonium chloride(NIPAM/APTMAC)binary DESs system was proposed to prepare intelligent MIPs with thermo-sensitivity.Accordingly,magnetic and thermo-responsive MIPs based on functional monomers-derived DESs(TMDESs-MIPs1)were synthesized,revealing DESs effect-regulated affinity/kinetics for the enhanced adsorption capability,eco-friendly thermo-regulated elution for high release efficiency,and simple magnetic separation,along with superior selectivity to rhein(RH)and good regeneration ability.TM-DESs-MIPs1 were utilized to extract RH from Cassiae semen samples coupled with high performance liquid chromatography(HPLC),yielding satisfactory recoveries(79.47%−110.82%)and low limits of detection(LOD)(16.67μg/L).Another two kinds of MIPs adopting the thermo-responsive moiety-derived DESs effect strategy further demonstrated great applicability of such intelligent MIPs for analyses of complicated samples.展开更多
基金supported by the National Natural Science Foundation of China(Grants Nos.12104406 and 12204105)the Natural Science Foundation Zhejiang Province,China(Grant No.ZCLMS25A0401)+1 种基金the Startup Grant of Zhejiang SciTech University(Grant No.21062338-Y)the Natural Science Foundation of Fujian Province,China(Grant No.2022J05116)。
文摘Phase imprinting enables the dynamic generation of superflow in bosonic atoms,effectively overcoming traditional limitations such as vortex number constraints and heating effects.However,the mechanisms underlying superflow formation remain insufficiently understood.In this work,we reveal these mechanisms by studying the time evolution of the transferred total angular momentum and the quantized current throughout the phase imprinting process,achieved through numerically solving the time-dependent Schrodinger and Gross-Pitaevskii equations.We demonstrate that the Bose gas dynamically acquires angular momentum through the density depletion induced by the phase imprinting potential,whereas quantized currents emerge from azimuthal phase slips accompanied by complete density depletions.Regarding the impact of system parameters,such as interactions,we find that interactions hinder superflow formation,as the azimuthal density distribution becomes less susceptible to the phase imprinting potential.Our findings offer microscopic insights into the dynamic development of superflow during the phase imprinting process and provide valuable guidance for ongoing experimental efforts.
基金supported by the Fuxiaquan Collaborative Innovation Platform(No.K30001)Major Scientific Research Program for Young and Middle-aged Health Professionals of Fujian Province,China(No.2022ZQNZD007)Youth Innovation Technology Project of Higher School in Shandong Province(Food Nanotechnology Innovation Team)。
文摘Quantitative determination of tetracycline(TC)in environment and foods is of great importance,as excessive residues might have negative effects on human health and environmental risks.Herein,a selfpowered molecularly imprinted photoelectrochemical(PEC)sensor based on the Zn O/C photoanode and the Fe-doped CuBi_(2)O_(4)(CBFO)photocathode is developed for the sensitive detection of TC.The photocathodic current can be amplified by the efficient electron transfer caused by the Fermi energy level gap between the photoanode and photocathode.Furthermore,molecularly imprinted polymers(MIPs)at photocathode can selectivity identify the TC templates and thus improve the specificity.Under the optimal conditions,the sensor has a linear range of 10^(-2)-1.0×10^(5) nmol/L,and a limit of detection(LOD)of 0.007 nmol/L(S/N=3).More crucially,the milk sample detection is carried out using the as-prepared sensor,and the outcome is satisfactory.The research gives us a novel sensing platform for quick and accurate antibiotic(like TC)in environment and food monitoring.
基金partially supported by the United States Department of Agriculture National Institute of Food and Agriculture Hatch Grant (Project No.OHO01304)。
文摘Background The primary differentially methylated regions(DMRs) which are maternally hypermethylated serve as imprinting control regions(ICRs) that drive monoallelic gene expression, and these ICRs have been investigated due to their implications in mammalian development. Although a subset of genes has been identified as imprinted, in-depth comparative approach needs to be developed for identification of species-specific imprinted genes. Here, we examined DNA methylation status and allelic expression at the KBTBD6 locus across species and tissues and explored potential mechanisms of imprinting.Results Using whole-genome bisulfite sequencing and RNA-sequencing on parthenogenetic and normal porcine embryos, we identified a maternally hypermethylated DMR between the embryos at the KBTBD6 promoter Cp G island and paternal monoallelic expression of KBTBD6. Also, in analyzed domesticated mammals but not in humans, non-human primates and mice, the KBTBD6 promoter Cp G islands were methylated in oocytes and/or allelically methyl-ated in tissues, and monoallelic KBTBD6 expression was observed, indicating livestock-specific imprinting. Further analysis revealed that these Cp G islands were embedded within transcripts in porcine and bovine oocytes which coexisted with an active transcription mark and DNA methylation, implying the presence of transcription-dependent imprinting.Conclusions In this study, our comparative approach revealed an imprinted expression of the KBTBD6 gene in domesticated mammals, but not in humans, non-human primates, and mice which implicates species-specific evolution of genomic imprinting.
基金supported by grants from the National Key Research and Development Program of China(2021YFA1101300,2021YFA1101800,and 2020YFA0112503)the National Natural Science Foundation of China(82030029,81970882,92149304,and 22302231)+5 种基金the Science and Technology Department of Sichuan Province(2021YFS0371)the Guangdong Basic and Applied Basic Research Foundation(2023A1515011986)the Shenzhen Fundamental Research Program(JCYJ20190814093401920,JCYJ20210324125608022,JCYJ20190813152616459,and JCYJ20190808120405672)the Futian Healthcare Research Project(FTWS2022013 and FTWS2023080)the Open Research Fund of State Key Laboratory of Genetic Engineering,Fudan University(SKLGE-2104)the Fundamental Research Funds for the Central Universities,Sun Yat-sen University(23qnpy153)。
文摘Copper is a microelement with important physiological functions in the body.However,the excess copper ion(Cu^(2+))may cause severe health problems,such as hair cell apoptosis and the resultant hearing loss.Therefore,the assay of Cu^(2+)is important.We integrate ionic imprinting technology(IIT)and structurally colored hydrogel beads to prepare chitosan-based ionically imprinted hydrogel beads(IIHBs)as a low-cost and high-specificity platform for Cu^(2+)detection.The IIHBs have a macroporous microstructure,uniform size,vivid structural color,and magnetic responsiveness.When incubated in solution,IIHBs recognize Cu^(2+)and exhibit a reflective peak change,thereby achieving label-free detection.In addition,benefiting from the IIT,the IIHBs display good specificity and selectivity and have an imprinting factor of 19.14 at 100μmol·L^(-1).These features indicated that the developed IIHBs are promising candidates for Cu^(2+)detection,particularly for the prevention of hearing loss.
基金National Key Research and Development Project(No.2019YFC1604904)National Natural Science Foundation of China(No.32101212)+1 种基金Natural Science Foundation of Jiangxi(No.20224ACB215009)Research Program of State Key Laboratory of Food Science and Technology in Nanchang University(No.SKLF-ZZB-202127).
文摘As a new concept having emerged in last few years,the“deep eutectic solvents”(DESs)effect integrated into the imprinting technology inevitably exposes design limitations of stimuli-responsive molecularly imprinted polymers(MIPs),as well as inadequate analysis of the adsorption performance of MIPs.Herein,a simple yet defined N-isopropylacrylamide/(3-acrylamidopropyl)trimethylammonium chloride(NIPAM/APTMAC)binary DESs system was proposed to prepare intelligent MIPs with thermo-sensitivity.Accordingly,magnetic and thermo-responsive MIPs based on functional monomers-derived DESs(TMDESs-MIPs1)were synthesized,revealing DESs effect-regulated affinity/kinetics for the enhanced adsorption capability,eco-friendly thermo-regulated elution for high release efficiency,and simple magnetic separation,along with superior selectivity to rhein(RH)and good regeneration ability.TM-DESs-MIPs1 were utilized to extract RH from Cassiae semen samples coupled with high performance liquid chromatography(HPLC),yielding satisfactory recoveries(79.47%−110.82%)and low limits of detection(LOD)(16.67μg/L).Another two kinds of MIPs adopting the thermo-responsive moiety-derived DESs effect strategy further demonstrated great applicability of such intelligent MIPs for analyses of complicated samples.
