[Objective] This study aimed to analyze the genetic relationships among Medicago sativa,Medicago falcata and Trigonella foenum-graecum.[Method] ISSR technique was adopted to determine their genetic relationships.[Resu...[Objective] This study aimed to analyze the genetic relationships among Medicago sativa,Medicago falcata and Trigonella foenum-graecum.[Method] ISSR technique was adopted to determine their genetic relationships.[Result] M.sativa,M.falcate and T.foenum-graecum had a broad genetic base.T.foenum-graecum shared closer relationship with M.falcata rather than M.sativa.The study on relationship between M.sativa and T.foenum-graecum was advantageous to identify disputable transition types.But a boundary should be found to identify species to be M.sativa or T.foenum-graecum.[Conclusion] This study will provide reference for identifying some disputable transition types.展开更多
Objective:To evaluate the effects of the standardized extract of fenugreek(Trigonella foenumgraecum L.Family:Leguminasae) seed(IND01) in animal models of peripheral neuropathy. Methods:IND01 was prepared from fenugree...Objective:To evaluate the effects of the standardized extract of fenugreek(Trigonella foenumgraecum L.Family:Leguminasae) seed(IND01) in animal models of peripheral neuropathy. Methods:IND01 was prepared from fenugreek seeds and standardized by high performance liquid chromatography to a marker compound,trigonelline.The effects of daily oral administration of IND01(50,100 and 200 mg/kg) were studied in rats after partial sciatic nerve ligation(PSNL) and sciatic nerve crush injury(SNCI) during 30-days period.The measurements on thermal hyperalgesia(TH),motor function test(MFT) score and motor nerve conduction velocity (MNCV) were recorded.Results:IND01 offered sustained protection against TH and deranged MFT scores in both models from 7-day onwards.Fifteen days of daily oral administration of IND01 restored MNCV reduction in rats with SNCI but not with PSNL.Conclusions:IND01 was found to be effective in rat models of painful peripheral neuropathy.展开更多
Aqueous extracts from various plant parts of fenugreek(3%)(aerial parts:leaves and stems(LS),roots(R),ground seeds(GS)and not ground seeds(NGS))and petroleum ether,ethyl acetate and methanolic fractions of the aerial ...Aqueous extracts from various plant parts of fenugreek(3%)(aerial parts:leaves and stems(LS),roots(R),ground seeds(GS)and not ground seeds(NGS))and petroleum ether,ethyl acetate and methanolic fractions of the aerial parts were assayed to determine their antifungal potential against Botrytis cinerea,Fusarium graminearum,Alternaria sp.,Pythium aphanidermatum,and Rhizoctinia solani.All fenugreek plant parts showed antifungal potential and the magnitude of their inhibitory effects was species and plant parts d...展开更多
The ability of seed extracts of Trigonella foenum-graecum (T. foenum-graecum) and Cuminum cyminum (C. cyminum) to act as natural coagulants was tested using natural turbid water. Seed extracts were prepared using dist...The ability of seed extracts of Trigonella foenum-graecum (T. foenum-graecum) and Cuminum cyminum (C. cyminum) to act as natural coagulants was tested using natural turbid water. Seed extracts were prepared using distilled water and NaCl (0.5 M and 1.0 M) solution. Only 1.0 M NaCl extract of T. foenum-graecum had coagulation capability and did not depend on pH values. Further it showed that natural coagulant obtained from T. foenum-graecum is temperature (upto 100?C) and pH stable (pH 4.0 - 10.0). Extract of C. cyminum had very minimal (16 ± 2) coagulation property. The seed extract of T. foenum-graecum showed about 80% coagulation properties, where as the best known natural coagulants such as Strychnos potatorum and Moringa oleifera, and chemical coagulant such as Al2(SO4)3 showed around 90%, 65% and 95% respectively, which are used as standards for the present study. When compared with pond water, T. foenum-graecum extract treated water shows decrease in alkalinity, turbidity, KMnO4 demand and total coli-form. This study reveals that seed extract of T. foenum-graecum can be used as natural water coagulant.展开更多
The aim of the study was to investigate distribution, enrichment and accumulation of heavy metals in soil and Trigonella foenum-graecum (var. Pusa Early Bunching) after fertigation with paper mill effluent. Doses of p...The aim of the study was to investigate distribution, enrichment and accumulation of heavy metals in soil and Trigonella foenum-graecum (var. Pusa Early Bunching) after fertigation with paper mill effluent. Doses of paper mill effluent viz. 5%, 10%, 25%, 50%, 75% and 100% were used for fertigation of T. foenum-graecum along with bore well water (control). The results revealed that paper mill effluent had significant (P +, K+, Ca2+, Mg2+, Fe2+, TKN, PO3-4, SO2-4, Cd, Cr, Cu, Mn and Zn of the soil in both seasons. Insignificant (P > 0.05) changes in WHC and bulk density of the soil were observed after irrigation with paper mill effluent. The agronomical performance of T. foenum-graecum was increased from 5% to 25% concentration and decreased from 50% to 100% concentration of paper mill effluent as compared to control in both seasons. The heavy metals concentration was increased in T. foenum-graecum from 5% to 100% concentrations of paper mill effluent in both seasons. Biochemical components like crude proteins, crude fiber and crude carbohydrates were found maximum with 25% paper mill effluent in both seasons. The enrichment factor (Ef) of various heavy metals was in order of Cd > Mn > Cr > Cu > Zn > Fe for soil and Mn > Cu > Cr > Cd > Zn > Fe for T. foenum-graecum plants after fertigation with paper mill effluent. Therefore, paper mill effluent can be used as a biofertigant after appropriate dilution to improve yield of T. foenum-graecum.展开更多
Objective: To assess the ameliorative properties of Iranian Trigonella foenum-graecum L.(T. foenum-graecum) seeds and Punica granatum L.(P. granatum) peel extracts against streptozotocin-induced diabetes in male guine...Objective: To assess the ameliorative properties of Iranian Trigonella foenum-graecum L.(T. foenum-graecum) seeds and Punica granatum L.(P. granatum) peel extracts against streptozotocin-induced diabetes in male guinea pigs.Methods: Sixty guinea pigs were divided into six groups(10 guinea pigs per group).Group 1 consisted of normal animals. Groups 2 and 3 were treated with Iranian T. foenum-graecum seeds and P. granatum peel extract alone, respectively. Group 4 was treated with streptozotocin only; whereas Groups 5 and 6 receiving streptozotocin were treated with Iranian T. foenum-graecum seeds and P. granatum peel extract, respectively.All animals were treated for 30 days, and the body weight, blood and liver biochemical parameters were measured.Results: Guinea pigs exposed to streptozotocin showed an alteration in body weight gain, fasting glucose level, kidney function parameters(blood urea nitrogen and creatinine) as well as decreased serum and hepatic total protein level. In addition, it increased the cholesterol and triglyceride level, while decreasing the hepatic glucose-6 phosphate dehydrogenase activity, glycogen, glutathione content and hepatic catalase activity. Oral treatment with T. foenum-graecum seeds and P. granatum peel extracts revealed significant protective properties with respect to body weight gain and other biochemical parameters studied.Conclusions: The Iranian T. foenum-graecum seeds and P. granatum peel extracts are significantly potent in ameliorating diabetic condition induced by streptozotocin and improving various biochemical parameters in serum and liver of guinea pigs.展开更多
The effects of cold atmospheric-pressure plasma jet (CAPPJ) were investigated on germination and early seedling growth of fenugreek (Trigonella foenum-graecum L) seeds. A two-electrode argon CAPPJ system with and with...The effects of cold atmospheric-pressure plasma jet (CAPPJ) were investigated on germination and early seedling growth of fenugreek (Trigonella foenum-graecum L) seeds. A two-electrode argon CAPPJ system with and without an additional grounded electrode [accelerating grounded (AG) electrode] was used at different exposure times. After 16 h of observation, the germination rates increased by 4 and 7 times, without and with using an AG electrode, respectively, for 1 min of plasma exposure. An increase in shoot fresh weight was observed, especially at 10 min exposure time. A high dry weight of root and shoot at 1 min-AG exposure time was observed. The root:shoot ratio was lower in plasma-treated seedlings, compared with the control plants. The study found that the O-radical emission line (777.4 nm) enhanced 5 times, due to the presence of an AG electrode, which increased the axial electric field and led to the formation of more streamers. The three stated effects (O-radicals, enhancement of the electric field and streamers) could be the cause for the stimulation of seed germination and seedling growth parameters when using the CAPPJ. The scanning electron microscope images showed the etching of the seed surface layers, which was more pronounced when an AG electrode was applied. The results of the current study indicate that the germination rates increased due to the increase of O-radical concentration and the etching of the seed surfaces.展开更多
[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 co...[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase:acetonitrile-0.1%glacial acetic acid;flow rate:1.0 mL/min;column temperature:30℃;detection wavelength:360 nm;sample injection volume:10μL.[Results]The kaempferol content in Yao medicine L.foenum-graecum was determined,Y=20652X-151098(R=0.9997)shows a good linear relationship within the linear range of 9.80-156.8μg/mL.[Conclusions]The high performance liquid chromatography(HPLC)method was established for determining the kaempferol content in Yao medicine L.foenum-graecum.This method is expected to provide a scientific basis for the formulation of quality control methods for L.foenum-graecum.展开更多
[Objectives]To establish a quality analysis method for Guangxi Zhuang medicine Lysimachia foenum-graecum Hance.[Methods]The moisture,total ash and alcohol-soluble extracts of L.foenum-graecum medicinal materials were ...[Objectives]To establish a quality analysis method for Guangxi Zhuang medicine Lysimachia foenum-graecum Hance.[Methods]The moisture,total ash and alcohol-soluble extracts of L.foenum-graecum medicinal materials were determined in accordance with the inspection method of the Chinese Pharmacopoeia(2015).[Results]The 10 batches of L.foenum-graecum medicinal materials from different origins and different collection times in Guangxi all had the same plant morphology and medicinal properties.The moisture content was 5.69%-10.51%,the total ash content was 5.50%-8.61%,the acid-insoluble ash content was 0.42%-0.78%,and the extract was 15.28%-20.75%.[Conclusions]This study is expected to provide a scientific basis for the development and utilization of L.foenum-graecum medicinal materials and the establishment of its quality standards.展开更多
基金Supported by Forage Germplasm Resource Protection Project of Ministry of Agriculture,China~~
文摘[Objective] This study aimed to analyze the genetic relationships among Medicago sativa,Medicago falcata and Trigonella foenum-graecum.[Method] ISSR technique was adopted to determine their genetic relationships.[Result] M.sativa,M.falcate and T.foenum-graecum had a broad genetic base.T.foenum-graecum shared closer relationship with M.falcata rather than M.sativa.The study on relationship between M.sativa and T.foenum-graecum was advantageous to identify disputable transition types.But a boundary should be found to identify species to be M.sativa or T.foenum-graecum.[Conclusion] This study will provide reference for identifying some disputable transition types.
文摘Objective:To evaluate the effects of the standardized extract of fenugreek(Trigonella foenumgraecum L.Family:Leguminasae) seed(IND01) in animal models of peripheral neuropathy. Methods:IND01 was prepared from fenugreek seeds and standardized by high performance liquid chromatography to a marker compound,trigonelline.The effects of daily oral administration of IND01(50,100 and 200 mg/kg) were studied in rats after partial sciatic nerve ligation(PSNL) and sciatic nerve crush injury(SNCI) during 30-days period.The measurements on thermal hyperalgesia(TH),motor function test(MFT) score and motor nerve conduction velocity (MNCV) were recorded.Results:IND01 offered sustained protection against TH and deranged MFT scores in both models from 7-day onwards.Fifteen days of daily oral administration of IND01 restored MNCV reduction in rats with SNCI but not with PSNL.Conclusions:IND01 was found to be effective in rat models of painful peripheral neuropathy.
文摘Aqueous extracts from various plant parts of fenugreek(3%)(aerial parts:leaves and stems(LS),roots(R),ground seeds(GS)and not ground seeds(NGS))and petroleum ether,ethyl acetate and methanolic fractions of the aerial parts were assayed to determine their antifungal potential against Botrytis cinerea,Fusarium graminearum,Alternaria sp.,Pythium aphanidermatum,and Rhizoctinia solani.All fenugreek plant parts showed antifungal potential and the magnitude of their inhibitory effects was species and plant parts d...
文摘The ability of seed extracts of Trigonella foenum-graecum (T. foenum-graecum) and Cuminum cyminum (C. cyminum) to act as natural coagulants was tested using natural turbid water. Seed extracts were prepared using distilled water and NaCl (0.5 M and 1.0 M) solution. Only 1.0 M NaCl extract of T. foenum-graecum had coagulation capability and did not depend on pH values. Further it showed that natural coagulant obtained from T. foenum-graecum is temperature (upto 100?C) and pH stable (pH 4.0 - 10.0). Extract of C. cyminum had very minimal (16 ± 2) coagulation property. The seed extract of T. foenum-graecum showed about 80% coagulation properties, where as the best known natural coagulants such as Strychnos potatorum and Moringa oleifera, and chemical coagulant such as Al2(SO4)3 showed around 90%, 65% and 95% respectively, which are used as standards for the present study. When compared with pond water, T. foenum-graecum extract treated water shows decrease in alkalinity, turbidity, KMnO4 demand and total coli-form. This study reveals that seed extract of T. foenum-graecum can be used as natural water coagulant.
文摘The aim of the study was to investigate distribution, enrichment and accumulation of heavy metals in soil and Trigonella foenum-graecum (var. Pusa Early Bunching) after fertigation with paper mill effluent. Doses of paper mill effluent viz. 5%, 10%, 25%, 50%, 75% and 100% were used for fertigation of T. foenum-graecum along with bore well water (control). The results revealed that paper mill effluent had significant (P +, K+, Ca2+, Mg2+, Fe2+, TKN, PO3-4, SO2-4, Cd, Cr, Cu, Mn and Zn of the soil in both seasons. Insignificant (P > 0.05) changes in WHC and bulk density of the soil were observed after irrigation with paper mill effluent. The agronomical performance of T. foenum-graecum was increased from 5% to 25% concentration and decreased from 50% to 100% concentration of paper mill effluent as compared to control in both seasons. The heavy metals concentration was increased in T. foenum-graecum from 5% to 100% concentrations of paper mill effluent in both seasons. Biochemical components like crude proteins, crude fiber and crude carbohydrates were found maximum with 25% paper mill effluent in both seasons. The enrichment factor (Ef) of various heavy metals was in order of Cd > Mn > Cr > Cu > Zn > Fe for soil and Mn > Cu > Cr > Cd > Zn > Fe for T. foenum-graecum plants after fertigation with paper mill effluent. Therefore, paper mill effluent can be used as a biofertigant after appropriate dilution to improve yield of T. foenum-graecum.
文摘Objective: To assess the ameliorative properties of Iranian Trigonella foenum-graecum L.(T. foenum-graecum) seeds and Punica granatum L.(P. granatum) peel extracts against streptozotocin-induced diabetes in male guinea pigs.Methods: Sixty guinea pigs were divided into six groups(10 guinea pigs per group).Group 1 consisted of normal animals. Groups 2 and 3 were treated with Iranian T. foenum-graecum seeds and P. granatum peel extract alone, respectively. Group 4 was treated with streptozotocin only; whereas Groups 5 and 6 receiving streptozotocin were treated with Iranian T. foenum-graecum seeds and P. granatum peel extract, respectively.All animals were treated for 30 days, and the body weight, blood and liver biochemical parameters were measured.Results: Guinea pigs exposed to streptozotocin showed an alteration in body weight gain, fasting glucose level, kidney function parameters(blood urea nitrogen and creatinine) as well as decreased serum and hepatic total protein level. In addition, it increased the cholesterol and triglyceride level, while decreasing the hepatic glucose-6 phosphate dehydrogenase activity, glycogen, glutathione content and hepatic catalase activity. Oral treatment with T. foenum-graecum seeds and P. granatum peel extracts revealed significant protective properties with respect to body weight gain and other biochemical parameters studied.Conclusions: The Iranian T. foenum-graecum seeds and P. granatum peel extracts are significantly potent in ameliorating diabetic condition induced by streptozotocin and improving various biochemical parameters in serum and liver of guinea pigs.
文摘The effects of cold atmospheric-pressure plasma jet (CAPPJ) were investigated on germination and early seedling growth of fenugreek (Trigonella foenum-graecum L) seeds. A two-electrode argon CAPPJ system with and without an additional grounded electrode [accelerating grounded (AG) electrode] was used at different exposure times. After 16 h of observation, the germination rates increased by 4 and 7 times, without and with using an AG electrode, respectively, for 1 min of plasma exposure. An increase in shoot fresh weight was observed, especially at 10 min exposure time. A high dry weight of root and shoot at 1 min-AG exposure time was observed. The root:shoot ratio was lower in plasma-treated seedlings, compared with the control plants. The study found that the O-radical emission line (777.4 nm) enhanced 5 times, due to the presence of an AG electrode, which increased the axial electric field and led to the formation of more streamers. The three stated effects (O-radicals, enhancement of the electric field and streamers) could be the cause for the stimulation of seed germination and seedling growth parameters when using the CAPPJ. The scanning electron microscope images showed the etching of the seed surface layers, which was more pronounced when an AG electrode was applied. The results of the current study indicate that the germination rates increased due to the increase of O-radical concentration and the etching of the seed surfaces.
基金Scientific Research and Technological Development Program Project of Hezhou City(He Ke Ji 20047)Scientific Research Program of Guangxi International Zhuang Medicine Hospital in 2020(GZ202001)+1 种基金Preparation Quality Improvement Project of Traditional Chinese Medicine Zhuang and Yao Medicine Hospital of Traditional Chinese Medicine Administration of Guangxi Zhuang Autonomous Region(GZZJ202013&GZZJ202015)Zhuang and Yao Medicine Research Laboratory of Guangxi International Zhuang Medicine Hospital.
文摘[Objectives]To determine the kaempferol content in Yao medicine Lysimachia foenum-graecum Hance.[Methods]The high performance liquid chromatography(HPLC)method was used.The chromatographic column:Inertsil ODS-3 C18 column(4.60 mm×250 mm,5μm);mobile phase:acetonitrile-0.1%glacial acetic acid;flow rate:1.0 mL/min;column temperature:30℃;detection wavelength:360 nm;sample injection volume:10μL.[Results]The kaempferol content in Yao medicine L.foenum-graecum was determined,Y=20652X-151098(R=0.9997)shows a good linear relationship within the linear range of 9.80-156.8μg/mL.[Conclusions]The high performance liquid chromatography(HPLC)method was established for determining the kaempferol content in Yao medicine L.foenum-graecum.This method is expected to provide a scientific basis for the formulation of quality control methods for L.foenum-graecum.
基金Key Research and Development Program of Department of Science and Technology of Guangxi Zhuang Autonomous Region(AB19110003)Scientific Research Program of Guangxi International Zhuang Medical Hospital in 2020(GZ202001)+2 种基金Project for Improving Basic Research Ability of Middle Aged and Young Teachers in Colleges and Universities of Guangxi in 2019(2019KY0341)Open Program of Key Laboratory of Zhuang and Yao Medicine(GXZYKF2020A-08)Youth Foundation Project of Guangxi University of Chinese Medicine(2019QN036).
文摘[Objectives]To establish a quality analysis method for Guangxi Zhuang medicine Lysimachia foenum-graecum Hance.[Methods]The moisture,total ash and alcohol-soluble extracts of L.foenum-graecum medicinal materials were determined in accordance with the inspection method of the Chinese Pharmacopoeia(2015).[Results]The 10 batches of L.foenum-graecum medicinal materials from different origins and different collection times in Guangxi all had the same plant morphology and medicinal properties.The moisture content was 5.69%-10.51%,the total ash content was 5.50%-8.61%,the acid-insoluble ash content was 0.42%-0.78%,and the extract was 15.28%-20.75%.[Conclusions]This study is expected to provide a scientific basis for the development and utilization of L.foenum-graecum medicinal materials and the establishment of its quality standards.
