[Objective] This study aimed to establish a new method for determination of anemoside B4 content in pulsatilla water extract. [Method] Using acetonitrile-water （28：72） as the mobile phase, the high performance liqu...[Objective] This study aimed to establish a new method for determination of anemoside B4 content in pulsatilla water extract. [Method] Using acetonitrile-water （28：72） as the mobile phase, the high performance liquid chromatography, equipped with UV detector, was used to determine the anemoside B4 content in pulsatilla water extract. [Result] In the concentration range of 300-800 μg/ml, anemoside B4 content showed a good linear relationship with peak area. The average recovery of anemoside B4 was 98.12% （n=-6; RSD=-1.37%）. [Conclusion] The established method meets the requirements by methodology, and it can be used to determine the anemoside B4 content in pulsatilla water extract.展开更多
Tillering is an important agronomic trait for rice grain production. To evaluate yield and tillering response, Liangyoupeijiu(super hybrid rice) was grown in Hunan, China during 2011–2012 under different methods of t...Tillering is an important agronomic trait for rice grain production. To evaluate yield and tillering response, Liangyoupeijiu(super hybrid rice) was grown in Hunan, China during 2011–2012 under different methods of tillage(conventional and no-tillage system) and crop establishment methods(transplanting at a spacing of 20 cm × 20 cm with one seedling per hill and direct seeding at a seeding rate of 22.5 kg ha-1). Our results revealed that, at maximum tillering(Max.) and at maturity(MA) stages, direct seeding(DS) resulted in 22% more tillers than transplanting(TP) irrespective of tillage system. Tiller mortality reached a peak between panicle initiation(PI) and booting(BT) stages, and was 16% higher under conventional tillage(CT) than under no-tillage(NT). Transplanting required 29% more time for the completion of tillering and less for DS. Tillering rate was 43% higher in DS than TP under either CT or NT. There was a positive correlation between panicle number per m2and maximum tiller number per m2, but not panicle-bearing tiller rate. The panicle bearing tiller rate was higher under DS than TP and higher under NT than CT. Tiller dry weight gradually increased up to heading(HD) stage, and was 14% higher under TP than DS. Leaf area(cm2tiller-1) gradually increased from Max. to HD stage and then decreased by 34% in conventional tillage transplanting(CTTP) and 45% in no-tillage transplanting(NTTP) from 12DAH–24DAH(days after heading), but was similar(35%) under DS under either CT or NT. Grain yield was higher under CTTP owing to the larger sink size(heavier panicle, more spikelets in per cm length of panicle) than under DS.展开更多
Objective To create an unique new method of digital orthopedic surgery and widely apply to spinal surgery,treatment of bone and joint injuries,ligament reconstruction and repair,bone minor resection and reconstruction...Objective To create an unique new method of digital orthopedic surgery and widely apply to spinal surgery,treatment of bone and joint injuries,ligament reconstruction and repair,bone minor resection and reconstruction,serious bone and展开更多
目的:基于化学发光免疫分析法及其中和试验,建立并评价用于乙型肝炎表面抗原(HBsAg)的确认方法。方法:①确认方法的检测程序:首先通过预试验确定中和抗体的最佳浓度。进行HBsAg确认时,在已加入测试血清的检测杯和对照杯中,按照1∶1比例(...目的:基于化学发光免疫分析法及其中和试验,建立并评价用于乙型肝炎表面抗原(HBsAg)的确认方法。方法:①确认方法的检测程序:首先通过预试验确定中和抗体的最佳浓度。进行HBsAg确认时,在已加入测试血清的检测杯和对照杯中,按照1∶1比例(100μl∶100μl)分别加入中和抗体和术前四项(乙肝血清学标志物、HCV抗体、梅毒螺旋体抗体、HIV抗体)全阴血清,混匀后,37℃下温育30 min,使用西门子Atellica IM HBsAg检测程序测试预处理的标本。②确认方法的可靠性评价:比对分析64例不同浓度HBsAg有反应性血清的确认方法检测结果与西门子确认试剂测试结果的符合率。③确认方法的诊断价值及阳性判断值的确定:以64例HBsAg有反应性标本的西门子确认试剂测试结果为“金标准”,应用ROC曲线统计分析确认方法检测结果的中和率。结果:①预试验中HBsAb≥1285.12 mU/ml时,检测结果中和率均>50%,中和率随着HBsAb浓度的升高而升高,不同浓度HBsAg经相同浓度HBsAb中和后,中和率变化趋势不明显。②两种方法最终结果的符合率为100.00%。③曲线下面积(AUC)为0.9934,阳性判断值为中和率≥47.93%,其敏感度为98.15%,特异度为100.00%。结论:该检测方法简单易行、经济实用、诊断价值高,适于不同浓度HBsAg的确认。展开更多
Patch-clamp technique serve as a powerful tool for precisely measuring and characterizing ion channel currents,offering critical molecular-level insights essential for drug screening and optimization.By enabling a dee...Patch-clamp technique serve as a powerful tool for precisely measuring and characterizing ion channel currents,offering critical molecular-level insights essential for drug screening and optimization.By enabling a deeper understanding of ion channel behavior,these techniques significantly accelerate the process of drug discovery and development.In recent years,automated patch-clamp technique has undergone substantial advancements,surpassing traditional manual methods with its high throughput,improved data consistency,and automation.However,fully harnessing these advantages requires meticulous optimization of experimental conditions tailored to specific targets.Without such refinement,the high cost of consumables and operational expenses could severely hinder the widespread adoption of this technique.This study focused on the TRPV1 channel,detailing the establishment of an automated patch-clamp detection system for TRPV1 currents,optimization of experimental parameters,and a comparative evaluation of the results against manual patch-clamp techniques.展开更多
该文旨在研究不同包装方式对杏与玫瑰花酱复合果丹皮在贮藏过程中品质变化的影响,并通过品质变化规律预测其货架期。首先采用糖纸包装(candy paper packaging,CW)、真空包装(vacuum packaging,VP)、保鲜袋包装(storage bag packaging,W...该文旨在研究不同包装方式对杏与玫瑰花酱复合果丹皮在贮藏过程中品质变化的影响,并通过品质变化规律预测其货架期。首先采用糖纸包装(candy paper packaging,CW)、真空包装(vacuum packaging,VP)、保鲜袋包装(storage bag packaging,WPB)和铝箔袋包装(aluminum foil bag packaging,ABP)4种包装方式对复合果丹皮进行包装,并在常温下贮藏180 d测定其在贮藏期间品质指标的变化值,以水分含量、可溶性糖含量、硬度和菌落总数为表征指标建立化学动力学模型以预测复合果丹皮的货架期。结果表明,采用VP方式能有效延缓复合果丹皮中可溶性糖、维生素C、活性成分等营养成分的降解、抑制复合果丹皮在贮藏期间被微生物的污染。可溶性糖和菌落总数的变化规律符合零级动力学模型、水分含量与硬度的变化规律符合一级动力学模型,验证结果表明,VP方式下复合果丹皮以4种表征指标建立的预测模型准确度较高,预测值与实测值相对误差较小,Arrhenius方程具有较好的拟合性。因此,VP方式的应用将会为复合果丹皮的包装和贮藏提供理论基础。展开更多
基金Supported by General Program of Beijing Municipal Education Commission(KM201410020007)~~
文摘[Objective] This study aimed to establish a new method for determination of anemoside B4 content in pulsatilla water extract. [Method] Using acetonitrile-water （28：72） as the mobile phase, the high performance liquid chromatography, equipped with UV detector, was used to determine the anemoside B4 content in pulsatilla water extract. [Result] In the concentration range of 300-800 μg/ml, anemoside B4 content showed a good linear relationship with peak area. The average recovery of anemoside B4 was 98.12% （n=-6; RSD=-1.37%）. [Conclusion] The established method meets the requirements by methodology, and it can be used to determine the anemoside B4 content in pulsatilla water extract.
基金financial supportprovided by the National Natural Science Foundation of China project, No. 311712494the financial support provided by NATP, BARC, Dhaka, Bangladesh
文摘Tillering is an important agronomic trait for rice grain production. To evaluate yield and tillering response, Liangyoupeijiu(super hybrid rice) was grown in Hunan, China during 2011–2012 under different methods of tillage(conventional and no-tillage system) and crop establishment methods(transplanting at a spacing of 20 cm × 20 cm with one seedling per hill and direct seeding at a seeding rate of 22.5 kg ha-1). Our results revealed that, at maximum tillering(Max.) and at maturity(MA) stages, direct seeding(DS) resulted in 22% more tillers than transplanting(TP) irrespective of tillage system. Tiller mortality reached a peak between panicle initiation(PI) and booting(BT) stages, and was 16% higher under conventional tillage(CT) than under no-tillage(NT). Transplanting required 29% more time for the completion of tillering and less for DS. Tillering rate was 43% higher in DS than TP under either CT or NT. There was a positive correlation between panicle number per m2and maximum tiller number per m2, but not panicle-bearing tiller rate. The panicle bearing tiller rate was higher under DS than TP and higher under NT than CT. Tiller dry weight gradually increased up to heading(HD) stage, and was 14% higher under TP than DS. Leaf area(cm2tiller-1) gradually increased from Max. to HD stage and then decreased by 34% in conventional tillage transplanting(CTTP) and 45% in no-tillage transplanting(NTTP) from 12DAH–24DAH(days after heading), but was similar(35%) under DS under either CT or NT. Grain yield was higher under CTTP owing to the larger sink size(heavier panicle, more spikelets in per cm length of panicle) than under DS.
文摘Objective To create an unique new method of digital orthopedic surgery and widely apply to spinal surgery,treatment of bone and joint injuries,ligament reconstruction and repair,bone minor resection and reconstruction,serious bone and
文摘目的:基于化学发光免疫分析法及其中和试验,建立并评价用于乙型肝炎表面抗原(HBsAg)的确认方法。方法:①确认方法的检测程序:首先通过预试验确定中和抗体的最佳浓度。进行HBsAg确认时,在已加入测试血清的检测杯和对照杯中,按照1∶1比例(100μl∶100μl)分别加入中和抗体和术前四项(乙肝血清学标志物、HCV抗体、梅毒螺旋体抗体、HIV抗体)全阴血清,混匀后,37℃下温育30 min,使用西门子Atellica IM HBsAg检测程序测试预处理的标本。②确认方法的可靠性评价:比对分析64例不同浓度HBsAg有反应性血清的确认方法检测结果与西门子确认试剂测试结果的符合率。③确认方法的诊断价值及阳性判断值的确定:以64例HBsAg有反应性标本的西门子确认试剂测试结果为“金标准”,应用ROC曲线统计分析确认方法检测结果的中和率。结果:①预试验中HBsAb≥1285.12 mU/ml时,检测结果中和率均>50%,中和率随着HBsAb浓度的升高而升高,不同浓度HBsAg经相同浓度HBsAb中和后,中和率变化趋势不明显。②两种方法最终结果的符合率为100.00%。③曲线下面积(AUC)为0.9934,阳性判断值为中和率≥47.93%,其敏感度为98.15%,特异度为100.00%。结论:该检测方法简单易行、经济实用、诊断价值高,适于不同浓度HBsAg的确认。
基金The National Natural Science Foundation of China(Grant No.32000674).
文摘Patch-clamp technique serve as a powerful tool for precisely measuring and characterizing ion channel currents,offering critical molecular-level insights essential for drug screening and optimization.By enabling a deeper understanding of ion channel behavior,these techniques significantly accelerate the process of drug discovery and development.In recent years,automated patch-clamp technique has undergone substantial advancements,surpassing traditional manual methods with its high throughput,improved data consistency,and automation.However,fully harnessing these advantages requires meticulous optimization of experimental conditions tailored to specific targets.Without such refinement,the high cost of consumables and operational expenses could severely hinder the widespread adoption of this technique.This study focused on the TRPV1 channel,detailing the establishment of an automated patch-clamp detection system for TRPV1 currents,optimization of experimental parameters,and a comparative evaluation of the results against manual patch-clamp techniques.
文摘该文旨在研究不同包装方式对杏与玫瑰花酱复合果丹皮在贮藏过程中品质变化的影响,并通过品质变化规律预测其货架期。首先采用糖纸包装(candy paper packaging,CW)、真空包装(vacuum packaging,VP)、保鲜袋包装(storage bag packaging,WPB)和铝箔袋包装(aluminum foil bag packaging,ABP)4种包装方式对复合果丹皮进行包装,并在常温下贮藏180 d测定其在贮藏期间品质指标的变化值,以水分含量、可溶性糖含量、硬度和菌落总数为表征指标建立化学动力学模型以预测复合果丹皮的货架期。结果表明,采用VP方式能有效延缓复合果丹皮中可溶性糖、维生素C、活性成分等营养成分的降解、抑制复合果丹皮在贮藏期间被微生物的污染。可溶性糖和菌落总数的变化规律符合零级动力学模型、水分含量与硬度的变化规律符合一级动力学模型,验证结果表明,VP方式下复合果丹皮以4种表征指标建立的预测模型准确度较高,预测值与实测值相对误差较小,Arrhenius方程具有较好的拟合性。因此,VP方式的应用将会为复合果丹皮的包装和贮藏提供理论基础。
