摘要
Patch-clamp technique serve as a powerful tool for precisely measuring and characterizing ion channel currents,offering critical molecular-level insights essential for drug screening and optimization.By enabling a deeper understanding of ion channel behavior,these techniques significantly accelerate the process of drug discovery and development.In recent years,automated patch-clamp technique has undergone substantial advancements,surpassing traditional manual methods with its high throughput,improved data consistency,and automation.However,fully harnessing these advantages requires meticulous optimization of experimental conditions tailored to specific targets.Without such refinement,the high cost of consumables and operational expenses could severely hinder the widespread adoption of this technique.This study focused on the TRPV1 channel,detailing the establishment of an automated patch-clamp detection system for TRPV1 currents,optimization of experimental parameters,and a comparative evaluation of the results against manual patch-clamp techniques.
膜片钳技术能够精确测量和分析离子通道蛋白的电流特性,为筛选和优化药物提供关键的分子层面信息,加速药物的发现和开发过程。近年来,全自动膜片钳技术逐渐发展,相比于手动膜片钳具有高通量、高质量和自动化的优点。然而,需要针对不同靶点将实验条件优化才能发挥其技术优势,否则昂贵的耗材费用等实验成本会大大限制该技术的发展。本研究以TRPV1通道为实验对象,介绍全自动膜片钳检测TRPV1通道电流的方法建立和参数优化,以及与手动膜片钳技术检测结果的对比分析。
基金
The National Natural Science Foundation of China(Grant No.32000674).
