摘要
血虚证是中医临床上常见的证型之一,由于人体中的血液无法滋养五脏六腑、经脉、四肢百骸,临床表现为面色无华、头晕目眩、心慌失眠等。临床上对于血虚证的诊断很大程度受医者个人经验和主观判断影响,使得血虚证的诊断难以统一和标准化,导致血虚证研究发展滞后。为推动血虚证的临床诊断标准化及研究进程,建立科学、合理、与中医临床实践相符合的血虚证动物模型及其评价体系尤为重要。血虚证动物模型的建立和评价指标的研究为中医药治疗血虚证提供了重要的实验基础。目前,血虚证动物模型主要通过物理、化学等手段损耗实验动物血液或抑制其造血功能来制备。本研究总结血虚证动物模型建立的常用方法,包括失血法、化学损伤法、溶血法、辐射损伤法和复合药物法,根据血虚证临床患者的症状,分析血虚证动物模型的评价指标,如一般体征、外周血象、骨髓造血功能、造血及免疫因子和多组学的生物标志物,以期提高后续血虚证相关研究的科学性和临床应用价值。
Blood deficiency syndrome is one of the most common clinical patterns in Traditional Chinese Medicine(TCM).It arises when insufficient blood fails to nourish the internal organs,meridians,and extremities,manifesting clinically as pallor,dizziness,palpi⁃tations,insomnia,and other symptoms.However,the diagnosis of blood deficiency syndrome in clinical practice remains heavily reliant on physicians´subjective judgment and individual experience,leading to inconsistencies in diagnostic criteria and hindering standardized research progress.To advance the standardization of clinical diagnosis and research on blood deficiency syndrome,it is crucial to estab⁃lish scientifically robust and clinically relevant animal models that align with TCM principles,along with a systematic evaluation frame⁃work.The development of these models and their evaluation metrics has laid an essential experimental foundation for TCM-based thera⁃pies targeting blood deficiency syndrome.Animal models of blood deficiency syndrome are primarily established through physical or chemical interventions that deplete blood or suppress hematopoietic function.This study summarizes common methodologies for construct⁃ing blood deficiency syndrome animal models,including blood loss,chemical injury,hemolysis,radiation injury,and compound drug administration.Furthermore,based on the clinical symptoms observed in patients,this review analyzes evaluation indicators for these models,such as general physical signs,peripheral blood parameters,bone marrow hematopoietic function,hematopoietic/immune fac⁃tors,and multi-omics biomarkers.These insights aim to enhance the scientific rigor and clinical applicability of future research on blood deficiency syndrome.
作者
陈晓莉
闫广利
CHEN Xiaoli;YAN Guangli(Heilongjiang University of Chinese Medicine,Heilongjiang Harbin 150006,China)
出处
《中国医药导刊》
2025年第3期232-238,共7页
Chinese Journal of Medicinal Guide
基金
河北省自然科学基金资助项目(项目编号:H2022106061,项目名称:基于中医方证代谢组学的古代经典名方中药新药基准物质的基础研究)。
关键词
血虚证
动物模型
建立方法
评价指标
Blood deficiency syndrome
Animal models
Establishment methods
Evaluation indicators
