The full-length cDNA sequence of prophenoloxidase was obtained through RACE technology. The complete cDNA sequence is 3 721-bp long, containing an open reading frame （ORF） of 1 881 bp, a 154-bp 5′-untranslated regi...The full-length cDNA sequence of prophenoloxidase was obtained through RACE technology. The complete cDNA sequence is 3 721-bp long, containing an open reading frame （ORF） of 1 881 bp, a 154-bp 5′-untranslated region, and a 1 686- bp 3′-untranslated region with three potential functional poly（A） signals （AATAAA）. The molecular mass of the deduced amino acid sequence （627 aa） was 72.3 kDa with an estimatedpI of 5.88. It contained putative copper-binding sites （copper A： 131, 135, 167 and copper B： 301,305, 341）, and a tentative complement-like motif （GCGWPDHL）. Eight potential N-linked glycosylation sites were predicted to be present in P. clarkii prophenoloxidase. Similar to those in other arthropod prophenoloxidases reported so far, no signal peptide was detected in the crayfish prophenoloxidase. The phylogenetic trees confirmed that P. clarkii prophenoloxidase was most closely related to that of freshwater crayfish P. leniusculus and more closely related to other crustacean prophenoloxidases from shrimp, prawn, and lobster than to the insect prophenoloxidases. Besides, two putative introns were found in this sequence of genomic DNA.展开更多
Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17or - hydroxyprogesterone, so as to investigating its effect on ovarian developmen...Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17or - hydroxyprogesterone, so as to investigating its effect on ovarian development red swamp crayfish （Procambarus clarkii）. The results showed that all the three differ- ent methods could facilitate the synchronous ovarian development of red swamp crayfish at different degree, with the degree of maturity of 10% high compared with the control group. The highest maturity degree was 50% that was observed in immersion treatment group. The effect was determined as in order immersion method 〉 injection method 〉 topical apphcation method 〉 control group. The immersion method and the injection method could facilitate synchronization spawning of red swamp crayfish, with the order of immersion method 32.5%, injection method 20%, topical application 17.5% and the control group 17.5 %. Taking the synchro- nization spawning rate, final spawning rate and mortality into account, the order was the immersion method 〉 injection method 〉 topical application method 〉 control group.展开更多
Procambarus clarkii is native to the south-central United States （Louisiana） and northeastern Mexico, and is a highly efficient predator that poses a damager to native species after its introduction or invasion. In ...Procambarus clarkii is native to the south-central United States （Louisiana） and northeastern Mexico, and is a highly efficient predator that poses a damager to native species after its introduction or invasion. In its natural habitat, P. clarkii consumes Carassius auratus, however, whether C. auratus recognizes P. clarkii as a predator is not yet clear. In laboratory experiments, we investigated whether experienced and inexperienced C. auratus recognize P. clarkii as a predatory threat and the specific sensory modality used by C. auratus to respond to chemical and visual stimuli from P. clarkii. In the chemical stimuli experiment, two kinds of chemical stimuli were used, water from a tub containing P. clarkii previously fed with C. auratus （C. auratus diet cues） and water from a tub containing unfed P. clarkii （P. clarkii cues）. In the visual experiment, experienced C. auratus decreased activity, but inexperienced C.auratus avoided the predator compartment. When C. auratus diet cues were presented, both experienced and inexperienced C. auratus increased the use of shelter, decreased activity in the initial response phase. Compared with the blank treatment, experienced C. auratus responded to P. clarkii cues by decreasing activity; however, inexperienced C. auratus showed no reduction in activity. C. auratus appears to recognize P. clarkii as a predator both through visual and chemical cues. Further analysis revealed that C. auratus may recognize R clarkii visually through the disturbances caused by P. clarkii movement and chemically by detecting conspecific alarm cues in the diet of P. clarkii. The results also indicate that ex- perienced C. auratus can recognize R clarkii by innate chemical cues from P. clarkii, whereas inexperienced C. auratus cannot [Current Zoology 57 （3）： 330-339, 2011].展开更多
The red swamp crayfish,Procambarus clarkii,is an economically important species especially in China.Their exoskeleton places serious constraints on growth and culture management.Their growth is achieved through interm...The red swamp crayfish,Procambarus clarkii,is an economically important species especially in China.Their exoskeleton places serious constraints on growth and culture management.Their growth is achieved through intermittent molting/ecdysis.The longitudinal genetic dynamics for growth-related traits at different ecdysial points in P.clarkii has been unclear to date.In this study,conditional genetic analysis was carried out for growth-related traits(body weight,body length,chela length,and cephalothorax length)based upon a mixed genetic model with conditional additive,dominance,and genotype by environment effects in P.clarkii.A complete diallel cross was made among three geographic populations of P.clarkii for the genetic mating design.Results of the conditional genetic analysis showed that from 4 th molt to 9 th molt the conditional additive variations were increased significantly whereas the conditional non-additive genetic variations(dominance and genotype by environment interaction)were decreased significantly for these growth-related traits.This indicated that lots of new expression of additive effect genes for body weight,body length,chela length,and cephalothorax length occurred during ontogeny,and environment played a signifi cant role in the expression of genes affecting these growth-related traits.Growth of the four traits was mainly affected by non-additive genetic effects in early developmental stage(prior to 4 th molt).The cumulative conditional additive variation for the growth-related traits from 4 th molt to 9 th molt accounted for a large majority of the total conditional additive variations from 2 nd molt to 9 th molt,indicating that this period was very important for the growth of this species.Using the conditional analysis method,dynamics of growth-related traits during an important ontogenetic phase of red swamp crayfish was uncovered.Our results provide valuable insights into refining production of this species.展开更多
Maternal care in crayfish has been extensively studied,but little is known of its response to environmental factors.We studied the duration of maternal care and its association with the juvenile development of marbled...Maternal care in crayfish has been extensively studied,but little is known of its response to environmental factors.We studied the duration of maternal care and its association with the juvenile development of marbled crayfish Procambarus virginalis in adverse environmental conditions.We exposed females with developmental stage 3 juveniles to environments of low water volume(LW)with food provided,low water volume with no feed(starvation)(LWS),and low water volume without feeding plus an intruder(red swamp crayfish male)(LWSI).A control group with standard water level/feed conditions was included for comparison.Groups were monitored for up to 90 days.The LWS group showed significantly prolonged maternal care(~65 days)as indicated by an extended attachment phase,delayed moulting of mothers(~53 days),and retarded juvenile development.In the LWSI group,conflict precluded extended maternal care.Low use of shelter and feeding was evident under all stress conditions.The results showed that crayfish females modify maternal care in accordance with conditions,providing a longer period of safety for offspring under adverse circumstances.It is possible that the expression of maternal pheromones can manipulate the developmental rate in juveniles,a topic for future research.展开更多
With the birth of Software-Defined Networking(SDN),integration of both SDN and traditional architectures becomes the development trend of computer networks.Network intrusion detection faces challenges in dealing with ...With the birth of Software-Defined Networking(SDN),integration of both SDN and traditional architectures becomes the development trend of computer networks.Network intrusion detection faces challenges in dealing with complex attacks in SDN environments,thus to address the network security issues from the viewpoint of Artificial Intelligence(AI),this paper introduces the Crayfish Optimization Algorithm(COA)to the field of intrusion detection for both SDN and traditional network architectures,and based on the characteristics of the original COA,an Improved Crayfish Optimization Algorithm(ICOA)is proposed by integrating strategies of elite reverse learning,Levy flight,crowding factor and parameter modification.The ICOA is then utilized for AI-integrated feature selection of intrusion detection for both SDN and traditional network architectures,to reduce the dimensionality of the data and improve the performance of network intrusion detection.Finally,the performance evaluation is performed by testing not only the NSL-KDD dataset and the UNSW-NB 15 dataset for traditional networks but also the InSDN dataset for SDN-based networks.Experimental results show that ICOA improves the accuracy by 0.532%and 2.928%respectively compared with GWO and COA in traditional networks.In SDN networks,the accuracy of ICOA is 0.25%and 0.3%higher than COA and PSO.These findings collectively indicate that AI-integrated feature selection based on the proposed ICOA can promote network intrusion detection for both SDN and traditional architectures.展开更多
基金financed by the Key Technology R&D Program from the Ministry of Science and Technology,China (2006BAD06A01)the Opening Subject of Hubei Key Lab of Animal Embryo & Molecular Breeding (2007ZD07)the Promoting Fund of Anhui Province Finance Department, China (05C1001)
文摘The full-length cDNA sequence of prophenoloxidase was obtained through RACE technology. The complete cDNA sequence is 3 721-bp long, containing an open reading frame （ORF） of 1 881 bp, a 154-bp 5′-untranslated region, and a 1 686- bp 3′-untranslated region with three potential functional poly（A） signals （AATAAA）. The molecular mass of the deduced amino acid sequence （627 aa） was 72.3 kDa with an estimatedpI of 5.88. It contained putative copper-binding sites （copper A： 131, 135, 167 and copper B： 301,305, 341）, and a tentative complement-like motif （GCGWPDHL）. Eight potential N-linked glycosylation sites were predicted to be present in P. clarkii prophenoloxidase. Similar to those in other arthropod prophenoloxidases reported so far, no signal peptide was detected in the crayfish prophenoloxidase. The phylogenetic trees confirmed that P. clarkii prophenoloxidase was most closely related to that of freshwater crayfish P. leniusculus and more closely related to other crustacean prophenoloxidases from shrimp, prawn, and lobster than to the insect prophenoloxidases. Besides, two putative introns were found in this sequence of genomic DNA.
文摘Three different treatment methods, including topical application method, injection method and immersion method were employed to treat 17or - hydroxyprogesterone, so as to investigating its effect on ovarian development red swamp crayfish （Procambarus clarkii）. The results showed that all the three differ- ent methods could facilitate the synchronous ovarian development of red swamp crayfish at different degree, with the degree of maturity of 10% high compared with the control group. The highest maturity degree was 50% that was observed in immersion treatment group. The effect was determined as in order immersion method 〉 injection method 〉 topical apphcation method 〉 control group. The immersion method and the injection method could facilitate synchronization spawning of red swamp crayfish, with the order of immersion method 32.5%, injection method 20%, topical application 17.5% and the control group 17.5 %. Taking the synchro- nization spawning rate, final spawning rate and mortality into account, the order was the immersion method 〉 injection method 〉 topical application method 〉 control group.
基金We would like to thank the undergradu- ate students from Guangxi Normal University, Yueni Huang, Chun Lan, and Caigui Nong, for their assistance in our experiment. This study was financially supported by Guangxi Science Foundation （2011GXNSFE018005）, Guangxi Key Laboratory of Environmental Engineering, Protection, and Assessment Kadoorie Farm ＆ Botanic Garden Corporation, Hong Kong and the Creative Team Project of the universities of Guangxi province, China.
文摘Procambarus clarkii is native to the south-central United States （Louisiana） and northeastern Mexico, and is a highly efficient predator that poses a damager to native species after its introduction or invasion. In its natural habitat, P. clarkii consumes Carassius auratus, however, whether C. auratus recognizes P. clarkii as a predator is not yet clear. In laboratory experiments, we investigated whether experienced and inexperienced C. auratus recognize P. clarkii as a predatory threat and the specific sensory modality used by C. auratus to respond to chemical and visual stimuli from P. clarkii. In the chemical stimuli experiment, two kinds of chemical stimuli were used, water from a tub containing P. clarkii previously fed with C. auratus （C. auratus diet cues） and water from a tub containing unfed P. clarkii （P. clarkii cues）. In the visual experiment, experienced C. auratus decreased activity, but inexperienced C.auratus avoided the predator compartment. When C. auratus diet cues were presented, both experienced and inexperienced C. auratus increased the use of shelter, decreased activity in the initial response phase. Compared with the blank treatment, experienced C. auratus responded to P. clarkii cues by decreasing activity; however, inexperienced C. auratus showed no reduction in activity. C. auratus appears to recognize P. clarkii as a predator both through visual and chemical cues. Further analysis revealed that C. auratus may recognize R clarkii visually through the disturbances caused by P. clarkii movement and chemically by detecting conspecific alarm cues in the diet of P. clarkii. The results also indicate that ex- perienced C. auratus can recognize R clarkii by innate chemical cues from P. clarkii, whereas inexperienced C. auratus cannot [Current Zoology 57 （3）： 330-339, 2011].
基金Supported by the National Natural Science Foundation of China(No.31672648)the Jiangsu Collaborative Innovation Center of Regional Modern Agriculture&Environmental Protection and Huaiyin Normal University(No.HSXT2-107)the Science&Technology Program of Huaiyin Normal University(No.31WH000)。
文摘The red swamp crayfish,Procambarus clarkii,is an economically important species especially in China.Their exoskeleton places serious constraints on growth and culture management.Their growth is achieved through intermittent molting/ecdysis.The longitudinal genetic dynamics for growth-related traits at different ecdysial points in P.clarkii has been unclear to date.In this study,conditional genetic analysis was carried out for growth-related traits(body weight,body length,chela length,and cephalothorax length)based upon a mixed genetic model with conditional additive,dominance,and genotype by environment effects in P.clarkii.A complete diallel cross was made among three geographic populations of P.clarkii for the genetic mating design.Results of the conditional genetic analysis showed that from 4 th molt to 9 th molt the conditional additive variations were increased significantly whereas the conditional non-additive genetic variations(dominance and genotype by environment interaction)were decreased significantly for these growth-related traits.This indicated that lots of new expression of additive effect genes for body weight,body length,chela length,and cephalothorax length occurred during ontogeny,and environment played a signifi cant role in the expression of genes affecting these growth-related traits.Growth of the four traits was mainly affected by non-additive genetic effects in early developmental stage(prior to 4 th molt).The cumulative conditional additive variation for the growth-related traits from 4 th molt to 9 th molt accounted for a large majority of the total conditional additive variations from 2 nd molt to 9 th molt,indicating that this period was very important for the growth of this species.Using the conditional analysis method,dynamics of growth-related traits during an important ontogenetic phase of red swamp crayfish was uncovered.Our results provide valuable insights into refining production of this species.
基金financially supported by the Grant Agency of the University of South Bohemia No.GAJU 027/2022/Z and 065/2022/Zmade using facilities of the large research infrastructure supported by the Ministry of Education,Youth and Sports of the Czech Republic project CENAKVA(LM2023038).
文摘Maternal care in crayfish has been extensively studied,but little is known of its response to environmental factors.We studied the duration of maternal care and its association with the juvenile development of marbled crayfish Procambarus virginalis in adverse environmental conditions.We exposed females with developmental stage 3 juveniles to environments of low water volume(LW)with food provided,low water volume with no feed(starvation)(LWS),and low water volume without feeding plus an intruder(red swamp crayfish male)(LWSI).A control group with standard water level/feed conditions was included for comparison.Groups were monitored for up to 90 days.The LWS group showed significantly prolonged maternal care(~65 days)as indicated by an extended attachment phase,delayed moulting of mothers(~53 days),and retarded juvenile development.In the LWSI group,conflict precluded extended maternal care.Low use of shelter and feeding was evident under all stress conditions.The results showed that crayfish females modify maternal care in accordance with conditions,providing a longer period of safety for offspring under adverse circumstances.It is possible that the expression of maternal pheromones can manipulate the developmental rate in juveniles,a topic for future research.
基金supported by the National Natural Science Foundation of China under Grant 61602162the Hubei Provincial Science and Technology Plan Project under Grant 2023BCB041.
文摘With the birth of Software-Defined Networking(SDN),integration of both SDN and traditional architectures becomes the development trend of computer networks.Network intrusion detection faces challenges in dealing with complex attacks in SDN environments,thus to address the network security issues from the viewpoint of Artificial Intelligence(AI),this paper introduces the Crayfish Optimization Algorithm(COA)to the field of intrusion detection for both SDN and traditional network architectures,and based on the characteristics of the original COA,an Improved Crayfish Optimization Algorithm(ICOA)is proposed by integrating strategies of elite reverse learning,Levy flight,crowding factor and parameter modification.The ICOA is then utilized for AI-integrated feature selection of intrusion detection for both SDN and traditional network architectures,to reduce the dimensionality of the data and improve the performance of network intrusion detection.Finally,the performance evaluation is performed by testing not only the NSL-KDD dataset and the UNSW-NB 15 dataset for traditional networks but also the InSDN dataset for SDN-based networks.Experimental results show that ICOA improves the accuracy by 0.532%and 2.928%respectively compared with GWO and COA in traditional networks.In SDN networks,the accuracy of ICOA is 0.25%and 0.3%higher than COA and PSO.These findings collectively indicate that AI-integrated feature selection based on the proposed ICOA can promote network intrusion detection for both SDN and traditional architectures.
