摘要
为研究水体中不同浓度的高效氯氰菊酯对克氏原螯虾抗氧化酶活性的影响,在本实验室测得高效氯氰菊酯致克氏原螯虾的96hLC50为0.2μg·L-1的基础上,将克氏原螯虾分别暴露于0、0.005、0.01、0.02、0.04μg·L-1的高效氯氰菊酯溶液中,在24、48、72h和96h后分别测定其鳃、肝胰腺和血清中超氧化物歧化酶(SOD)和过氧化氢酶(CAT)的活性以及丙二醛(MDA)的含量。结果表明,在染毒96h期间各组织中的SOD活性变化规律相似,均为先上升后下降;肝胰腺中的CAT活性先下降后上升再下降,而鳃和血清中的CAT活性表现为先上升后下降;丙二醛(MDA)含量则一直高于对照组,随着暴露时间和暴露浓度的增加而增加。染毒96h后,0.04μg·L-1处理组与空白对照组相比,肝胰腺、鳃和血清中的SOD活性分别下降了28.3%、44.1%和27.2%,CAT活性分别下降了38.1%、39.4%和12.3%,而MDA含量分别较空白对照组提高了0.72、1.09倍和0.46倍。研究结果提示,高效氯氰菊酯对克氏原螯虾的抗氧化酶活性具有显著的抑制效应。
Beta-cypermethrin, a type 11 pyrethroid insecticide, is widely used in agriculture around the world. Although much research on the toxicity of beta-cypermethrin has been done in fishes, little is so far available regarding its toxicity to crustaceans, such as shrimp, crab and crayfish. To investigate the acute toxic effect of beta-cypermethrin on Procambarus clarkii, the crayfishes were exposed to various con- centrations of beta-cypermethrin（0 t^g＂ L-1, 0.005 Ixg＂ L-1, 0.01 Ixg＂ L-~, 0.02 ~g＂ L-~ and 0.04 Ixg＂ L-＇ ） until 96 h aecording to the 96 h LCs0 values （0.2 Ixg＂ L-1） measured previously. Activities of antioxidant enzymes viz. superoxide dismutase （SOD） and catalase （CAT）, and con- tents of malondialdehyde（MDA ） in gill, hepatopancreas and serum were determined at 24 h, 48 h, 72 h and 96 h, respectively. Results indi- cated that SOD activities first increased then declined in different tissues during the 96 h exposure, and that CAT activities showed the similar trends. MDA contents in all treatments were higher than those in the control group at all the time and they increased with the exposed time and concentration increasing. After 96 h, the activities of SOD in hepatopancreas, gill and serum at 0.04 p,g. L-l treatment decreased by 28.3%, 44.1% and 27.2%, compared with control group, respectively. Similarly, the activities of CAT in different tissues decreased by 38.1%, 39.4% and 12.3%, respectively. MDA contents in hepatopancreas, gill and serum were 0.72 times, 1.09 times and 0.46 times higher than those in the control group, respectively. This study showed that beta-cypermethrin was able to induce oxidative stress and had significant negative effects on antioxidant enzyme activities of Procambarus clarkii.
出处
《农业环境科学学报》
CAS
CSCD
北大核心
2013年第4期689-696,共8页
Journal of Agro-Environment Science
基金
教育部高等学校博士学科点专项科研基金(200801081012)
山西省人才引进与开发专项(2008)
