As COP30 concluded in Belém,Brazil,China emerged as one of the most visible and influential players in global climate discussions.Over the course of the summit,the Chinese delegation demonstrated both diplomatic ...As COP30 concluded in Belém,Brazil,China emerged as one of the most visible and influential players in global climate discussions.Over the course of the summit,the Chinese delegation demonstrated both diplomatic skill and technological leadership,positioning the country as a key architect of practical climate solutions.展开更多
目的:探讨2种肝癌细胞BEL-7402细胞及BEL-7402/FU细胞中差异表达的miRNA并对其基因表达谱进行生物学信息分析。方法:采用TRIzol一步法提取BEL-7402细胞及BEL-7402/FU细胞的总RNA,并纯化mRNA,反转录合成荧光分子Hy3标记的cDNA探针,与基...目的:探讨2种肝癌细胞BEL-7402细胞及BEL-7402/FU细胞中差异表达的miRNA并对其基因表达谱进行生物学信息分析。方法:采用TRIzol一步法提取BEL-7402细胞及BEL-7402/FU细胞的总RNA,并纯化mRNA,反转录合成荧光分子Hy3标记的cDNA探针,与基因芯片杂交;采用Genepix Pro 6.0图像分析软件对芯片图像进行分析,把图像信号转化为数字信号,然后以差异为2倍的标准来确定差异表达基因。结果:在333个基因表达谱的筛选中,发现有2个基因表达水平显著上调,331个基因表达水平显著下调。结论:miR-122,miR-195等基因组对表达肝癌细胞耐药基因表达谱有显著的影响,可能参与肝癌耐药的发生、发展。展开更多
文摘As COP30 concluded in Belém,Brazil,China emerged as one of the most visible and influential players in global climate discussions.Over the course of the summit,the Chinese delegation demonstrated both diplomatic skill and technological leadership,positioning the country as a key architect of practical climate solutions.
文摘目的:探讨2种肝癌细胞BEL-7402细胞及BEL-7402/FU细胞中差异表达的miRNA并对其基因表达谱进行生物学信息分析。方法:采用TRIzol一步法提取BEL-7402细胞及BEL-7402/FU细胞的总RNA,并纯化mRNA,反转录合成荧光分子Hy3标记的cDNA探针,与基因芯片杂交;采用Genepix Pro 6.0图像分析软件对芯片图像进行分析,把图像信号转化为数字信号,然后以差异为2倍的标准来确定差异表达基因。结果:在333个基因表达谱的筛选中,发现有2个基因表达水平显著上调,331个基因表达水平显著下调。结论:miR-122,miR-195等基因组对表达肝癌细胞耐药基因表达谱有显著的影响,可能参与肝癌耐药的发生、发展。
