OBJECTIVE: To investigate the immunomodulatory mechanism by which Yangfei Huoxue decoction(YHD) alleviates bleomycin(BLM)-induced pulmonary fibrosis(PF) in rats.METHODS: Rats were randomly divided into two time-point ...OBJECTIVE: To investigate the immunomodulatory mechanism by which Yangfei Huoxue decoction(YHD) alleviates bleomycin(BLM)-induced pulmonary fibrosis(PF) in rats.METHODS: Rats were randomly divided into two time-point groups(day 14 and 28), and each timepoint group comprised the following six subgroups: control, BLM, dexamethasone(DXM), YHD high dose(YHD-H), YHD middle dose(YHD-M), and YHD low dose(YHD-L). Haematoxylin and eosin and Masson staining, flow cytometry, enzymelinked immunosorbent assay, Western blotting and UPLC-QT of analyses were examined.RESULTS: The results showed that YHD reduced the degree of alveolar inflammation and fibrosis;downregulated the expression of CD28, CD80,CD86, Delta-like 1, Notch2, and Notch3;and upregulated the proportions of Th1/Th2 and Tc1/Tc2. The seven components of YHD were detected.CONCLUSION: The current study indicates that YHD mainly functions by regulating the immune system and that the molecular mechanism may be related to the regulation of the Notch signaling pathway.展开更多
Objective： The present study, for the first time, was designed to examine the antifibrosis effects of Shenge Yangfei Capsules（SGYF） on bleomycin-induced pulmonary injury and fibrosis in rats.Methods： Animals were ...Objective： The present study, for the first time, was designed to examine the antifibrosis effects of Shenge Yangfei Capsules（SGYF） on bleomycin-induced pulmonary injury and fibrosis in rats.Methods： Animals were divided into six groups randomly： saline control group, model group（bleomycin5 mg/kg）, and positive control group（Dexamethasone 5 mg/kg）, and SGYF（70, 420, and 850 mg/kg/d, ig）group. Effects of SGYF on body weight and lung index were evaluated. For histological evaluation of lungs injury and fibrosis, lung specimens were stained with hematoxylin and eosin（HE） and Masson trichrome.Body weight and lung index from various groups were measured, and the concentrations of monoamine（MAO） and transforming growth factor-β1（TGF-β1） were also detected in lung homogenates.Results： SGYF attenuated the bleomycin-induced weight loss and the lung index. Histologica levidence showed the ability of SGYF to decrease bleomycin-induced lung fibrosis and consolidation. SGYF reduced MAO and TGF-β1 activity in lung tissues.Conclusions： These findings suggest that SGYF may be a promising candidate to prevent bleomycininduced lung damage or other interstitial lesions.展开更多
基金Supported by the National Natural Science Foundation of China(Based on Notch Signaling Pathway and Its Epigenetic Changes to Discuss the Mechanism of Nourishing Lung and Promoting Blood Circulation Formula to Prevent and Treat Pulmonary Fibrosis,No.81373603)。
文摘OBJECTIVE: To investigate the immunomodulatory mechanism by which Yangfei Huoxue decoction(YHD) alleviates bleomycin(BLM)-induced pulmonary fibrosis(PF) in rats.METHODS: Rats were randomly divided into two time-point groups(day 14 and 28), and each timepoint group comprised the following six subgroups: control, BLM, dexamethasone(DXM), YHD high dose(YHD-H), YHD middle dose(YHD-M), and YHD low dose(YHD-L). Haematoxylin and eosin and Masson staining, flow cytometry, enzymelinked immunosorbent assay, Western blotting and UPLC-QT of analyses were examined.RESULTS: The results showed that YHD reduced the degree of alveolar inflammation and fibrosis;downregulated the expression of CD28, CD80,CD86, Delta-like 1, Notch2, and Notch3;and upregulated the proportions of Th1/Th2 and Tc1/Tc2. The seven components of YHD were detected.CONCLUSION: The current study indicates that YHD mainly functions by regulating the immune system and that the molecular mechanism may be related to the regulation of the Notch signaling pathway.
基金financially supported by Guanghua Fund(No.413FZ08)
文摘Objective： The present study, for the first time, was designed to examine the antifibrosis effects of Shenge Yangfei Capsules（SGYF） on bleomycin-induced pulmonary injury and fibrosis in rats.Methods： Animals were divided into six groups randomly： saline control group, model group（bleomycin5 mg/kg）, and positive control group（Dexamethasone 5 mg/kg）, and SGYF（70, 420, and 850 mg/kg/d, ig）group. Effects of SGYF on body weight and lung index were evaluated. For histological evaluation of lungs injury and fibrosis, lung specimens were stained with hematoxylin and eosin（HE） and Masson trichrome.Body weight and lung index from various groups were measured, and the concentrations of monoamine（MAO） and transforming growth factor-β1（TGF-β1） were also detected in lung homogenates.Results： SGYF attenuated the bleomycin-induced weight loss and the lung index. Histologica levidence showed the ability of SGYF to decrease bleomycin-induced lung fibrosis and consolidation. SGYF reduced MAO and TGF-β1 activity in lung tissues.Conclusions： These findings suggest that SGYF may be a promising candidate to prevent bleomycininduced lung damage or other interstitial lesions.
