摘要
目的:探讨养肺活血方对平阳霉素致肺纤维化大鼠肺部炎症的影响。方法:大鼠麻醉后,经气管软骨环间隙向心端穿刺,注入平阳霉素5 mg.kg-1建立大鼠肺纤维化模型,假手术组大鼠在同样麻醉条件下气管内注入等量生理盐水。术后第2天开始每天灌胃给药,假手术组和模型组给予0.5%羧甲基纤维素钠(CMC钠)溶液,地塞米松组给予等容积地塞米松蒸馏水溶液,活血组给予等容积活血方合剂,养肺活血组给予等容积养肺活血方合剂。灌胃28 d,称取体重,麻醉后颈动脉取血处死大鼠,称肺重,计算肺指数。血液离心取血清检测白细胞介素-4(IL-4),肿瘤坏死因子-α(TNF-α),干扰素-γ(IFN-γ)观察对炎症因子的影响。左肺固定,包埋切片,常规脱蜡,行HE染色,观察肺泡炎症程度。免疫组化SABC法检测肺组织转化生长因子-β(TGF-β),结缔组织生长因子(CTGF)的水平,采用光学显微镜和全自动图像获取系统随机测量并计算平均灰度值,观察养肺活血方对肺纤维化大鼠肺部炎症的影响。结果:养肺活血方可显著减轻平阳霉素诱发肺纤维化大鼠肺泡炎症程度及评分(P<0.01),显著降低血IL-4,TNF-α,IFN-γ水平(P<0.01),显著增加肺组织TGF-β,CTGF的灰度(P<0.01),对肺指数无明显影响。结论:养肺活血方可显著减轻平阳霉素诱发肺纤维化大鼠的肺泡炎症程度,而降低血IL-4,TNF-α,IFN-γ水平和影响肺组织TGF-β,CTGF的水平可能是其作用机制之一。
Objective： To investigate whether Yangfei Huoxue prescription can inhibit lung inflammation of Pingyangmycin （PYM） -induced pulmonary fibrosis in rats. Method： PYM （5 mg ·kg^（-1） ） was instilled into the rat trachea with a microliter injector to induce pulmonary fibrosis. Rats were randomLy divided into five groups： sham-operated group, control group, dexamethasone group, Huoxue group and Yangfei Huoxue group. Sham operated group and control group were given 0.5% carboxymethyl cellulose （CMC sodium） solution 1 mL/100 g weight. Dexamethasone group was given equal volume of dexamethasone solution. Huoxue group was given equal volume of Huoxue prescription. Yangfei Huoxue group was given equal volume of Yangfei Huoxue prescription for consecutive 28 days. The rats were anesthetized and killed by carotid exsanguination to calculate the lung index based on lung weight and body weight. The blood was separated at 3 000 r · min^（-1） fot 10 min and obtained serum to examine IL-4, TNF-a and IFN-γ by radioimmunoassay. Left lungs were fixed, embedded, and 4um sections were prepared. Sections were stained with hematoxylin/eosin to assay alveolitis and score. TGF-beta and CTGF of the lung tissue were inspected by immunohistochemistry SABC and calculated the average gray scale. Result： Yangfei Huoxue prescription can significantly reduce the degree of alveolar inflammation and IL-4, TNF-a, IFN-γ in blood of PYM-induced lung injury in rats. Yangfei Huoxue prescription can significantly increase the gray scale of TGF-beta and CTGF. Conclusion： Yangfei Huoxue prescription can inhibit lung inflammation and PYM- induced pulmonary fibrosis in rats.
出处
《中国实验方剂学杂志》
CAS
北大核心
2012年第21期179-184,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
江苏省博士后项目(1102170C)
江苏省高校自然科学基础研究面上项目(08KJB360008)
关键词
肺纤维化
养肺活血方
炎症因子
肺部炎症
Pulmonary Fibrosis
Yangfei Huoxue prescription
Inflammatory cytokines
alveolarinflammation
