目的:探讨Plac8l1(placenta-specific 8 like 1)基因在小鼠睾丸组织的表达特征,及其与精子发生的相关性。方法:经生物信息学方法初步筛选到一个睾丸特异性表达候选基因Plac8l1,并推测其可能与小鼠精子发生相关;采用半定量RT-PCR和实时定...目的:探讨Plac8l1(placenta-specific 8 like 1)基因在小鼠睾丸组织的表达特征,及其与精子发生的相关性。方法:经生物信息学方法初步筛选到一个睾丸特异性表达候选基因Plac8l1,并推测其可能与小鼠精子发生相关;采用半定量RT-PCR和实时定量RT-PCR对Plac8l1表达的时空特异进行了研究;应用原位杂交技术对Plac8l1 m RNA在成年小鼠睾丸中的细胞类群表达特征进行初步研究;通过构建过表达PLAC8L1-EGFP重组蛋白质粒及生物信息学分析进行亚细胞定位研究与功能预测。结果:(1)Plac8l1是1个睾丸特异性转录基因。(2)在成年小鼠,Plac8l1只在睾丸和附睾组织发生特异性转录,而且睾丸中的表达水平明显高于附睾[睾丸vs.附睾=[(1.000±0.000)vs.(0.036±0.018),P=0.000]。(3)随着小鼠精子发生的起始,睾丸中Plac8l1转录水平逐渐升高(P=0.000)。(4)m RNA原位杂交结果显示Plac8l1 m RNA主要定位在成年小鼠睾丸中的间质细胞和精母细胞。(5)经生物信息学分析,PLAC8L1也有着一个类似于PLAC8的功能域,过表达重组蛋白显示PLAC8L1在细胞膜上聚集。结论:Plac8l1是1个睾丸特异性基因,可能与精子发生过程中的精母细胞分化相关;PLAC8L1具有1个富含半胱氨酸的PLAC8结构域,这一结构域可能介导了PLAC8L1在细胞膜上聚集并与其他蛋白相互作用,并可能在精母细胞分化中发挥重要作用。展开更多
Placenta specific protein 8 （PLAC 8） also called onzin or C15, is a cys- teine-rich protein. It is widely distributed in eukaryotes, and its function has been extensively studied. It not only regulates cell division...Placenta specific protein 8 （PLAC 8） also called onzin or C15, is a cys- teine-rich protein. It is widely distributed in eukaryotes, and its function has been extensively studied. It not only regulates cell division, differentiation, and apoptosis, but also plays an important role in the immune process. This article describes the latest progress of plac8 gene structure characteristics, evolution, expression and function and possible mechanisms.展开更多
胎盘特异蛋白8(Placenta special gene 8,plac8)又称onzin,是一种富含半胱氨酸,有着独特结构并在组织中特异性表达的蛋白。Plac8广泛分布于真核生物中,可以调节细胞一系列的生理活动,包括增殖、分裂、分化、免疫和凋亡等。除此之外,plac...胎盘特异蛋白8(Placenta special gene 8,plac8)又称onzin,是一种富含半胱氨酸,有着独特结构并在组织中特异性表达的蛋白。Plac8广泛分布于真核生物中,可以调节细胞一系列的生理活动,包括增殖、分裂、分化、免疫和凋亡等。除此之外,plac8还通过不同的信号通路在肿瘤发生分化中发挥着重要的作用。拟对plac8的结构、表达、功能和可能的作用机制进行综合性论述。展开更多
胎盘特异性蛋白8(the protein placenta-specific 8,PLAC8),又被称为Onzin和C15,是一种富含半胱氨酸的蛋白。PLAC8与其他蛋白具有很少的序列相似性,其功能得到越来越广泛的研究,特别是其调控肿瘤细胞发生、发展的作用。目前研究发现PLAC...胎盘特异性蛋白8(the protein placenta-specific 8,PLAC8),又被称为Onzin和C15,是一种富含半胱氨酸的蛋白。PLAC8与其他蛋白具有很少的序列相似性,其功能得到越来越广泛的研究,特别是其调控肿瘤细胞发生、发展的作用。目前研究发现PLAC8可以调控肿瘤细胞的细胞凋亡、细胞周期、细胞分化和上皮-间质转化及细胞自噬等过程。本文旨在对PLAC8在肿瘤发病中的作用及机制进行综述。展开更多
胎盘特异蛋白8(placenta special gene 8,plac8)又称Onzin或C15,是一种富含半胱氨酸的蛋白质,广泛分布于真核生物中。plac8可影响细胞分化以及凋亡过程,同时在多种疾病尤其是肿瘤的发生过程中具有重要作用。本文旨在阐述plac8的结构特...胎盘特异蛋白8(placenta special gene 8,plac8)又称Onzin或C15,是一种富含半胱氨酸的蛋白质,广泛分布于真核生物中。plac8可影响细胞分化以及凋亡过程,同时在多种疾病尤其是肿瘤的发生过程中具有重要作用。本文旨在阐述plac8的结构特点、功能以及其可能存在的作用机制等。展开更多
Background:Placenta specific 8(PLAC8)is a candidate oncogene involved in the development and progression of solid tumors.However,the status of PLAC8 in lung cancer(LC),especially non-small cell lung cancer(NSCLC)is st...Background:Placenta specific 8(PLAC8)is a candidate oncogene involved in the development and progression of solid tumors.However,the status of PLAC8 in lung cancer(LC),especially non-small cell lung cancer(NSCLC)is still not lucid.Methods:Tissue microarray analysis(TMA)was performed to detect the expression patterns of PLAC8 in LC tissues and cell lines.Then a series of cellular experiments were performed fto assess cell proliferation,cell cycle profiles,and cell motility to explore the role of PLAC8 in NSCLC-derived cell lines:H1299 and A549.Results:TMA results showed that PLAC8 played complex and even contradictory roles in different LC samples.Detailed cell-based experiments confirmed that PLAC8 could promote cell viability,alter the cell cycle,and accelerate cell mobility by regulating cell cyclins or cadherins,respectively.Conclusions:The study findings indicate that PLAC8 might participate in LC,especially NSCLC,by affecting cellular physiological processes.The outcomes also shed new light on the potential role of PLAC8 as a therapeutic biomarker in NSCLC.展开更多
The spatiotemporal relationships in high-resolution during odontogenesis remain poorly understood.We report a cell lineage and atlas of developing mouse teeth.We performed a large-scale(92,688 cells)single cell RNA se...The spatiotemporal relationships in high-resolution during odontogenesis remain poorly understood.We report a cell lineage and atlas of developing mouse teeth.We performed a large-scale(92,688 cells)single cell RNA sequencing,tracing the cell trajectories during odontogenesis from embryonic days 10.5 to 16.5.Combined with an assay for transposase-accessible chromatin with high-throughput sequencing,our results suggest that mesenchymal cells show the specific transcriptome profiles to distinguish the tooth types.Subsequently,we identified key gene regulatory networks in teeth and bone formation and uncovered spatiotemporal patterns of odontogenic mesenchymal cells.CD24^(+)and Plac8^(+)cells from the mesenchyme at the bell stage were distributed in the upper half and preodontoblast layer of the dental papilla,respectively,which could individually induce nonodontogenic epithelia to form tooth-like structures.Specifically,the Plac8^(+)tissue we discovered is the smallest piece with the most homogenous cells that could induce tooth regeneration to date.Our work reveals previously unknown heterogeneity and spatiotemporal patterns of tooth germs that may lead to tooth regeneration for regenerative dentistry.展开更多
文摘目的:探讨Plac8l1(placenta-specific 8 like 1)基因在小鼠睾丸组织的表达特征,及其与精子发生的相关性。方法:经生物信息学方法初步筛选到一个睾丸特异性表达候选基因Plac8l1,并推测其可能与小鼠精子发生相关;采用半定量RT-PCR和实时定量RT-PCR对Plac8l1表达的时空特异进行了研究;应用原位杂交技术对Plac8l1 m RNA在成年小鼠睾丸中的细胞类群表达特征进行初步研究;通过构建过表达PLAC8L1-EGFP重组蛋白质粒及生物信息学分析进行亚细胞定位研究与功能预测。结果:(1)Plac8l1是1个睾丸特异性转录基因。(2)在成年小鼠,Plac8l1只在睾丸和附睾组织发生特异性转录,而且睾丸中的表达水平明显高于附睾[睾丸vs.附睾=[(1.000±0.000)vs.(0.036±0.018),P=0.000]。(3)随着小鼠精子发生的起始,睾丸中Plac8l1转录水平逐渐升高(P=0.000)。(4)m RNA原位杂交结果显示Plac8l1 m RNA主要定位在成年小鼠睾丸中的间质细胞和精母细胞。(5)经生物信息学分析,PLAC8L1也有着一个类似于PLAC8的功能域,过表达重组蛋白显示PLAC8L1在细胞膜上聚集。结论:Plac8l1是1个睾丸特异性基因,可能与精子发生过程中的精母细胞分化相关;PLAC8L1具有1个富含半胱氨酸的PLAC8结构域,这一结构域可能介导了PLAC8L1在细胞膜上聚集并与其他蛋白相互作用,并可能在精母细胞分化中发挥重要作用。
基金Supported by National Natural Science Foundation of China(31172074,30700071)Shandong Provincial Natural Science Foundation(ZR2010CL002)~~
文摘Placenta specific protein 8 （PLAC 8） also called onzin or C15, is a cys- teine-rich protein. It is widely distributed in eukaryotes, and its function has been extensively studied. It not only regulates cell division, differentiation, and apoptosis, but also plays an important role in the immune process. This article describes the latest progress of plac8 gene structure characteristics, evolution, expression and function and possible mechanisms.
文摘胎盘特异蛋白8(Placenta special gene 8,plac8)又称onzin,是一种富含半胱氨酸,有着独特结构并在组织中特异性表达的蛋白。Plac8广泛分布于真核生物中,可以调节细胞一系列的生理活动,包括增殖、分裂、分化、免疫和凋亡等。除此之外,plac8还通过不同的信号通路在肿瘤发生分化中发挥着重要的作用。拟对plac8的结构、表达、功能和可能的作用机制进行综合性论述。
文摘胎盘特异性蛋白8(the protein placenta-specific 8,PLAC8),又被称为Onzin和C15,是一种富含半胱氨酸的蛋白。PLAC8与其他蛋白具有很少的序列相似性,其功能得到越来越广泛的研究,特别是其调控肿瘤细胞发生、发展的作用。目前研究发现PLAC8可以调控肿瘤细胞的细胞凋亡、细胞周期、细胞分化和上皮-间质转化及细胞自噬等过程。本文旨在对PLAC8在肿瘤发病中的作用及机制进行综述。
文摘胎盘特异蛋白8(placenta special gene 8,plac8)又称Onzin或C15,是一种富含半胱氨酸的蛋白质,广泛分布于真核生物中。plac8可影响细胞分化以及凋亡过程,同时在多种疾病尤其是肿瘤的发生过程中具有重要作用。本文旨在阐述plac8的结构特点、功能以及其可能存在的作用机制等。
基金supported by the Fund for Key Laboratory Construction of Hubei Province(Grant No.2018BFC360)the National Natural Science Foundation of China(Grant No.31101047 to Lu Xue)“the Fundamental Research Funds for the Central Universities”,South-Central Minzu University(Grant Number:CZQ22013).
文摘Background:Placenta specific 8(PLAC8)is a candidate oncogene involved in the development and progression of solid tumors.However,the status of PLAC8 in lung cancer(LC),especially non-small cell lung cancer(NSCLC)is still not lucid.Methods:Tissue microarray analysis(TMA)was performed to detect the expression patterns of PLAC8 in LC tissues and cell lines.Then a series of cellular experiments were performed fto assess cell proliferation,cell cycle profiles,and cell motility to explore the role of PLAC8 in NSCLC-derived cell lines:H1299 and A549.Results:TMA results showed that PLAC8 played complex and even contradictory roles in different LC samples.Detailed cell-based experiments confirmed that PLAC8 could promote cell viability,alter the cell cycle,and accelerate cell mobility by regulating cell cyclins or cadherins,respectively.Conclusions:The study findings indicate that PLAC8 might participate in LC,especially NSCLC,by affecting cellular physiological processes.The outcomes also shed new light on the potential role of PLAC8 as a therapeutic biomarker in NSCLC.
基金supported by the National Key Research and Development Program of China Stem Cell and Translational Research,China(2017YFA0104800)the Research Funds from Health@InnoHK Program launched by Innovation Technology Commission of the Hong Kong SAR,China+4 种基金National Natural Science Foundation of China(81570944 and 92068201)Science and Technology Planning Project of Guangdong Province,China(2020B1212060052)High-level Hospital Construction Project(DFJHBF202110)Youth Innovation Promotion of the Chinese Academy of Sciences(2019348)Guangzhou Key Medical Disciplines(2021–2023)。
文摘The spatiotemporal relationships in high-resolution during odontogenesis remain poorly understood.We report a cell lineage and atlas of developing mouse teeth.We performed a large-scale(92,688 cells)single cell RNA sequencing,tracing the cell trajectories during odontogenesis from embryonic days 10.5 to 16.5.Combined with an assay for transposase-accessible chromatin with high-throughput sequencing,our results suggest that mesenchymal cells show the specific transcriptome profiles to distinguish the tooth types.Subsequently,we identified key gene regulatory networks in teeth and bone formation and uncovered spatiotemporal patterns of odontogenic mesenchymal cells.CD24^(+)and Plac8^(+)cells from the mesenchyme at the bell stage were distributed in the upper half and preodontoblast layer of the dental papilla,respectively,which could individually induce nonodontogenic epithelia to form tooth-like structures.Specifically,the Plac8^(+)tissue we discovered is the smallest piece with the most homogenous cells that could induce tooth regeneration to date.Our work reveals previously unknown heterogeneity and spatiotemporal patterns of tooth germs that may lead to tooth regeneration for regenerative dentistry.
