Insulin resistance(IR)is a central event in the pathogenesis of type 2 diabetes mellitus(T2DM).In recent years,the global burden of T2DM has intensified,with conventional therapeutic agents carrying certain side effec...Insulin resistance(IR)is a central event in the pathogenesis of type 2 diabetes mellitus(T2DM).In recent years,the global burden of T2DM has intensified,with conventional therapeutic agents carrying certain side effects.Therefore,there is an urgent need to identify safer and more effective alternative therapies,which has accelerated research on natural green plants.Lonicera caerulea var.edulis is abundant in diverse bioactive constituents,however,the hypoglycemic potential of its important bioactive component—polysaccharides—has not yet been fully exploited.Within this investigation,a native polysaccharide(LP)was separated from L.caerulea var.edulis.Structural analysis revealed that LP was an acidic heteropolysaccharide possessing a molecular weight of 109.64 kDa,consisting mainly of galacturonic acid,galactose,and arabinose.Additionally,LP contained both HG and RG-I domains and showed a porous layered structure with favorable thermal stability.In insulin resistant HepG2(IR-HepG2)cell model,LP ameliorated insulin resistance,as evidenced by increased glucose consumption(up to 1.39-fold),glycogen synthesis(up to 2.34-fold),and enhanced activities of hexokinase(up to 1.48-fold)and pyruvate kinase(up to 1.98-fold)relative to the model group,through modulation of the IRS-1/PI3K/Akt signaling pathway.Notably,at concentrations of 100-200μg/mL,LP demonstrated significant hypoglycemic effects,markedly improving these metabolic parameters and thereby alleviating insulin resistance.These findings confirm LP's capability to regulate T2DM,establishing a scientific basis for the prospective use of L.caerulea var.edulis as a promising functional food resource.展开更多
The main components of Artemisia leucophylla(Turcz.ex Bess.) C.B.Clarke extract are volatile oils,flavonoids,lignans,terpenoids,etc.Studies have shown that A.leucophylla has bactericidal,anti-inflammatory,anti-oxidant...The main components of Artemisia leucophylla(Turcz.ex Bess.) C.B.Clarke extract are volatile oils,flavonoids,lignans,terpenoids,etc.Studies have shown that A.leucophylla has bactericidal,anti-inflammatory,anti-oxidant,anti-ultraviolet functions,and it has large development space and application value in many fields.This paper first introduced the growth resource distribution,active component extraction method,composition and efficacy of A.leucophylla,and analyzed the application prospects in daily necessities,in order to provide reference for the in-depth research,development and utilization of A.leucophylla.展开更多
In order to solve the problems including low fruit-bearing rate and low yield caused by blind plantation in production, the medium formula for pollen germination of Lonicera caerulea L. var. edulis Turcz. ex Herd., it...In order to solve the problems including low fruit-bearing rate and low yield caused by blind plantation in production, the medium formula for pollen germination of Lonicera caerulea L. var. edulis Turcz. ex Herd., its pollination habit, self-compatibility and open pollination compatibility between different superior individuals were investigated with superior individuals from superior wild individuals of L. caerulea in Changbai Mountains as experimental materials. Self-compatibility and open pollination compatibility among different fine plants provide theoretical basis for its further application and further selection of new varieties. The results showed the optimal medium for germination of L. caerulea pollen was 27.5% sucrose ＋ 100 PPM boric acid with a pH value of 6.0. On this medium, the superior individuals L1, L2 and L3 had the pollen germination rates of 53.4%, 50.9% and 51.6%, respectively. The three superior individuals had no significant differences in the quantity of germinated pollen tubes, which ranged from 1 to 4. The three excellent single plants were the most likely to germinate a single pollen tube, accounting for 83.3% of the pollen germinated and 89.6% of the pollen germinated. Therefore, in production, it is necessary to plant L1 with a certain amount of pollination trees or plant it together with multiple species, to improve its yield. On the contrary, L2 and L3 have no need for pollination trees theoretically, but whether the fruits obtained by self-pollination and outcrossing differ in quality still needs further study.展开更多
基金supported by the National Key Research and Development Program of China(2023YFF1104001).
文摘Insulin resistance(IR)is a central event in the pathogenesis of type 2 diabetes mellitus(T2DM).In recent years,the global burden of T2DM has intensified,with conventional therapeutic agents carrying certain side effects.Therefore,there is an urgent need to identify safer and more effective alternative therapies,which has accelerated research on natural green plants.Lonicera caerulea var.edulis is abundant in diverse bioactive constituents,however,the hypoglycemic potential of its important bioactive component—polysaccharides—has not yet been fully exploited.Within this investigation,a native polysaccharide(LP)was separated from L.caerulea var.edulis.Structural analysis revealed that LP was an acidic heteropolysaccharide possessing a molecular weight of 109.64 kDa,consisting mainly of galacturonic acid,galactose,and arabinose.Additionally,LP contained both HG and RG-I domains and showed a porous layered structure with favorable thermal stability.In insulin resistant HepG2(IR-HepG2)cell model,LP ameliorated insulin resistance,as evidenced by increased glucose consumption(up to 1.39-fold),glycogen synthesis(up to 2.34-fold),and enhanced activities of hexokinase(up to 1.48-fold)and pyruvate kinase(up to 1.98-fold)relative to the model group,through modulation of the IRS-1/PI3K/Akt signaling pathway.Notably,at concentrations of 100-200μg/mL,LP demonstrated significant hypoglycemic effects,markedly improving these metabolic parameters and thereby alleviating insulin resistance.These findings confirm LP's capability to regulate T2DM,establishing a scientific basis for the prospective use of L.caerulea var.edulis as a promising functional food resource.
基金Supported by Project of National Natural Science Foundation of China(21503072)Project of China Scholarship Council(20163035)+2 种基金Project of Introduction of Return Talents(XDB-2017-19)Student’s Platform for Innovation and Entrepreneurship Training Program at State Level(201710223002)Guiding Science and Technology Program of Daqing City(ZD-2017-59)
文摘The main components of Artemisia leucophylla(Turcz.ex Bess.) C.B.Clarke extract are volatile oils,flavonoids,lignans,terpenoids,etc.Studies have shown that A.leucophylla has bactericidal,anti-inflammatory,anti-oxidant,anti-ultraviolet functions,and it has large development space and application value in many fields.This paper first introduced the growth resource distribution,active component extraction method,composition and efficacy of A.leucophylla,and analyzed the application prospects in daily necessities,in order to provide reference for the in-depth research,development and utilization of A.leucophylla.
基金Supported by the Cooperative Project between the Yanbian University,China and National Institute of Horticultural and Herbal Science of the Rural Development Administration(RDA)of the Republic of Korea
文摘In order to solve the problems including low fruit-bearing rate and low yield caused by blind plantation in production, the medium formula for pollen germination of Lonicera caerulea L. var. edulis Turcz. ex Herd., its pollination habit, self-compatibility and open pollination compatibility between different superior individuals were investigated with superior individuals from superior wild individuals of L. caerulea in Changbai Mountains as experimental materials. Self-compatibility and open pollination compatibility among different fine plants provide theoretical basis for its further application and further selection of new varieties. The results showed the optimal medium for germination of L. caerulea pollen was 27.5% sucrose ＋ 100 PPM boric acid with a pH value of 6.0. On this medium, the superior individuals L1, L2 and L3 had the pollen germination rates of 53.4%, 50.9% and 51.6%, respectively. The three superior individuals had no significant differences in the quantity of germinated pollen tubes, which ranged from 1 to 4. The three excellent single plants were the most likely to germinate a single pollen tube, accounting for 83.3% of the pollen germinated and 89.6% of the pollen germinated. Therefore, in production, it is necessary to plant L1 with a certain amount of pollination trees or plant it together with multiple species, to improve its yield. On the contrary, L2 and L3 have no need for pollination trees theoretically, but whether the fruits obtained by self-pollination and outcrossing differ in quality still needs further study.
