OBJECTIVE:To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤,XXMD)in the treatment of Parkinson’s disease(PD).METHODS:C57BL/6 mice were randomly divided into ...OBJECTIVE:To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤,XXMD)in the treatment of Parkinson’s disease(PD).METHODS:C57BL/6 mice were randomly divided into eight groups(n=12),including blank group,model group,medication group,acupuncture group,high-dose XXMD group(XXMD-H),low-dose XXMD group(XXMD-L),acupuncture combined with high-dose XXMD group(A+H),and acupuncture combined with low-dose XXMD group(A+L).After treatment for 6 weeks,dopamine(DA)neurons and the pathological changes of tyrosine hydroxylase(TH)positive cells were observed.The enzyme-linked immunosorbent assay(ELISA)was used to measure the content of DA and the level of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor alpha(TNF-α).The m RNA level of PINK1 and Parkin and the protein expression of Nix,PINK1 and Parkin in the substantia nigra were also detected.RESULTS:Combination treatment effectively ameliorated the symptoms of PD.Compared with model group,combined treatment significantly up-regulated the protein expression of Nix,Parkin and PINK1 and the m RNA levels of PINK1 and Parkin in the substantia nigra(P<0.0001,P<0.001,P<0.01 or P<0.05).Furthermore,the levels of pro-inflammation cytokines were obviously decreased after combination therapy,while IL-10 content was increased remarkably(P<0.01).CONCLUSION:Compared with each treatment alone,combination therapy improved the pathological damage of DA neurons of PD mice more effectively.The possible mechanism may be attributed to the up-regulated level of mitochondrial autophagy and improved mitochondrial function.These results provide fresh insight into the mechanism of co-treatment with Tongdu Tiaoshen acupuncture and XXMD for PD.展开更多
Objective:To observe the impacts of the combined treatment of Tongdu Tiaoshen moxibustion(moxibustion for promoting the circulation of the governor vessel and regulating the spirit)and rehabilitation training on the m...Objective:To observe the impacts of the combined treatment of Tongdu Tiaoshen moxibustion(moxibustion for promoting the circulation of the governor vessel and regulating the spirit)and rehabilitation training on the motor function recovery in the patients with apoplectic hemiplegia.Methods:A total of 50 patients with apoplectic hemiplegia and qualified in the trial recruitment criteria were divided into two groups according to random number table,25 cases in each group.In the control group,the simple rehabilitation training was provided.In the observation group,on the base of the treatment as the control group,Tongdu Tiaoshen moxibustion was given.Main acupoints:Baihul(百会GV20),Fengfu(风府GV16)and Dazhui(大椎GV14).The treatment was given once a day,6 treatments a week,4 weeks as one course and two courses of treatment were required.Before and after treatment,the scores of Fugle-Meyer assessment(FMA),the modified Barthel index(MBI)and action research arm test(ARAT)were detected before and after treatment in the two groups separately.Results:After 4 and 8 weeks of treatment,the scores of FMA,MBI and ARAT were all improved obviously as compared with those before treatment respectively in patients of the two groups(all P<0.01).After8 weeks of treatment,the score of each scale in the observation group was more obviously improved as compared with the control group(all P<0.05).The difference in the clinical therapeutic effect was significant statistically between the two groups(P=0.005).Conclusion:The combined treatment of Tongdu Tiaoshen moxibustion and rehabilitation training promotes the recovery of the upper limb motor function and improves the self-ability of living activities in the patients with apoplectic hemiplegia.The long-term effect of this combined therapy is superior to that of simple rehabilitation training.展开更多
Lumbar spinal stenosis is caused by the compression of the nerve root or cauda equina nerve by stenosis of the lumbar spinal canal or intervertebral foramen,and is manifested as chronic low back and leg pain.Danlu Ton...Lumbar spinal stenosis is caused by the compression of the nerve root or cauda equina nerve by stenosis of the lumbar spinal canal or intervertebral foramen,and is manifested as chronic low back and leg pain.Danlu Tongdu(DLTD)tablets can relieve chronic pain caused by lumbar spinal stenosis,but the molecular mechanism remains largely unknown.In this study,the potential molecular mechanism of DLTD tablets in the treatment of lumbar spinal stenosis was first predicted by the network pharmacology method.Results showed that DLTD functions in regulating anti-oxidative,apoptosis,and inflammation signaling pathways.Furthermore,the flow cytometry results showed that DLTD tablets efficiently reduced reactive oxygen species content and inhibited rat neural stem cell apoptosis induced by hydrogen peroxide.DLTD also inhibited the mitochondrial membrane potential damage induced by hydrogen peroxide.Elisa analysis showed that DLTD induced cell cycle-related protein,CDK2 and CDK4,and reduced CDKN1A protein expression level.Taken together,our study provided new insights of DLTD in treating lumbar spinal stenosis through reducing reactive oxygen species content,decreasing apoptosis by inhibiting CDKN1A and promoting CDK2 and CDK4 expression levels.展开更多
[Objectives]To evaluate the effects of Tongdu Tiaoshen Acupuncture method on improving learning and memory function and hippocampal nerve regeneration and repair after stroke.[Methods]Animal models of cognitive impair...[Objectives]To evaluate the effects of Tongdu Tiaoshen Acupuncture method on improving learning and memory function and hippocampal nerve regeneration and repair after stroke.[Methods]Animal models of cognitive impairment in the recovery phase of cerebral infarction in rats were prepared,and rats were randomly divided into control group,model group,donepezil group,and acupuncture group.Morris water maze experiment was carried out to evaluate learning and memory ability.Nissl staining,BrdU and DCX immunofluorescence staining were used to observe the number and morphological structure of neuronal pyramidal and granular cells in the dentate gyrus(DG)area of the hippocampus,as well as the proliferation and differentiation of neural progenitor cells.TUNEL method was used to detect cell apoptosis.[Results]Before intervention,the scores and ELP of the model group,donepezil group and acupuncture group were significantly higher than those of the blank group(P<0.05);after the intervention,the neurological scores and ELP of the blank group and the model group were not significantly different from those before the intervention(P<0.05);the donepezil group and acupuncture group were significantly reduced,there was no difference between the donepezil group and acupuncture group(P>0.05),but it was still significantly higher than the blank group(P<0.05).The results of BrdU and DCX immunofluorescence staining showed that there were only a few BrdU positive cells and DCX protein in the hippocampal DG area of mice in the blank group,and the positive cells in the model group were significantly increased(P<0.05);donepezil group and acupuncture group showed significant increase compared with the model group(P<0.05);compared with the blank group,the number of neurons in the hippocampal DG area of each group was significantly reduced(P<0.01),and the neuronal apoptosis index was significantly increased(P<0.01);compared with the blank group,the number of neurons in the hippocampal DG area of each group was significantly reduced(P<0.01),and the neuronal apoptosis index was significantly increased(P<0.01).The number of neurons in the hippocampal DG area of rats in the acupuncture group and donepezil group was significantly increased(P<0.01),and the neuronal apoptosis index was significantly decreased(P<0.01).[Conclusions]Tongdu Tiaoshen Acupuncture method can improve the learning and memory function of stroke model rats by promoting the regeneration and repair of hippocampal nerve in rats with cerebral infarction.展开更多
Spinal disorders are diseases caused by congenital, degenerative, postural errors, trauma, and external evils that result in lesions of bones, discs, ligaments, and muscles, thus compressing the nerves. Spine-related ...Spinal disorders are diseases caused by congenital, degenerative, postural errors, trauma, and external evils that result in lesions of bones, discs, ligaments, and muscles, thus compressing the nerves. Spine-related diseases include scoliosis, intervertebral disc herniation, intervertebral stenosis, spinal fracture, ankylosing spondylitis, spinal cord injury, spinal tumour, etc., which seriously affect people’s daily life. This paper summarises the relevant studies on the treatment of spinal diseases by Tongzhu therapy. The mechanism of action of Tongzhu therapy in treating spinal diseases is closely related to the control of inflammatory factors, the improvement of local microcirculation, analgesia, and the promotion of nerve repair. This paper describes the application and modern mechanism of Tongzhu therapy in the treatment of spinal diseases, which will provide a direction for future research on Tongzhu therapy.展开更多
Objective To observe the effects of Tongdu Tiaoshen acupuncture(for unblocking the obstruction in the governor vessel and regulating the spirit)on depressionlike behavior and the hippocampal Endophilin A1/reactive oxy...Objective To observe the effects of Tongdu Tiaoshen acupuncture(for unblocking the obstruction in the governor vessel and regulating the spirit)on depressionlike behavior and the hippocampal Endophilin A1/reactive oxygen species(ROS)pathway in the chronic unpredictable mild stress(CUMS)model rats,and explore the mechanism of this therapy for depression.Methods Forty-eight male SD rats of SPF grade were randomly divided into a normal group(n=12) and a modeling group(n=36).展开更多
Objective To explore the metabolomic changes and possible mechanism of Tongdu Xingshen electroacupuncture in alleviating middle cerebral artry occlusion/reperfusion(MCAO/R) learning and memory impairment rats.Methods ...Objective To explore the metabolomic changes and possible mechanism of Tongdu Xingshen electroacupuncture in alleviating middle cerebral artry occlusion/reperfusion(MCAO/R) learning and memory impairment rats.Methods A total of 90 SD rats were divided into a control group(n=15),a sham group(n=15),and 60 MCAOR rat models were established by thread suppositing method.展开更多
Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord in...Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord injury,thereby improving the infiammatory microenvironment after spinal cord injury.Methods Fifty C57BL/6 mice were divided into 5 groups by random number table method:sham operation group,model group,deferoxamine(DFO)group,WSTDR group,WSTDR+DFO group,10 mice in each group.A mouse model of spinal cord injury was prepared by modified Allen's method.The pathological changes of the spinal cord were observed by HE staining and Nissl staining.The iron ion content in serum was determined by the iron ion colorimetric assay kit.The expression levels of monocyte chemoattractant protein-1(MCP-1),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in the homogenate and serum of the spinal cord were detected by ELISA.The phagocytosis and clearance of myelin debris by MECs in the spinal cord were observed by 3D laser confocal.Results One mouse died in the model group,DFO group,and WSTDR group,respectively.The results of HE staining showed that compared with the model group,spinal cord cavities were reduced with nerve fiber disorder in DFO group and WSTDR group.Nissl staining analysis showed that compared with the model group,Nissl morphology and neuronal pyknosis were improved in DFO group and WSTDR group.Ferric ion colorimetric assay showed that compared with the model group,serum iron ion concentration were significantly reduced in DFO group and WSTDR group(P<0.01).ELISA results showed that compared with the sham-operation group,the levels of MCP-1,IL-6,IL-1β,and TNF-αin serum and spinal cord tissues significantly increased in the model group(P<0.01).Compared with the model group,serum levels of MCP-1,IL-6,IL-1β,and TNF-αwere significantly lowered in DFO group and WSTDR group(P<0.05,P<0.01).Serum concentrations of MCP-1 and IL-1βwere significantly lowered in WSTDR+DFO group(P<0.01).Compared with WSTDR group,serum concentrations of MCP-1,IL-6,anddIL-1βwere significantly improved in WSTDR+DFO group(P<0.01).Compared with the model group,the levels of MCP-1,IL-6,IL-1β(except WSTDR group),and TNF-αin spinal cord tissues were significantly reduced in DFO group,WSTDR group,and WSTDR+DFO group(P<0.05,P<0.01).3D laser confocal results showed MECs phagocytosis and clearance of myelin debris were promoted in DFO group and WSTDR group.Conclusion WSTDR promotes MECs to engulf myelin debris by reducing iron overload,improving the infiammatory microenvironment,and promoting spinal cord injury repairment.展开更多
基金Supported by National Natural Science Foundation of China:Study on the Mechanism of Regulating miR-124 by Tongdu Tiaoshen Acupuncture to Promote Neuroprotection in Cerebral Ischemia Reperfusion Injury(No.81973933)Natural Science Fund for Colleges and Universities in Anhui Province:Study on the Regulatory Mechanism of Tongdu Tiaoshen Acupuncture on Dopaminergic Neurons in Parkinson’s Disease Model Mice Based on Mitochondrial Autophagy(No.KJ2019A0475)。
文摘OBJECTIVE:To explore the possible mechanism of Tongdu Tiaoshen acupuncture combined with Xiaoxuming decoction(小续命汤,XXMD)in the treatment of Parkinson’s disease(PD).METHODS:C57BL/6 mice were randomly divided into eight groups(n=12),including blank group,model group,medication group,acupuncture group,high-dose XXMD group(XXMD-H),low-dose XXMD group(XXMD-L),acupuncture combined with high-dose XXMD group(A+H),and acupuncture combined with low-dose XXMD group(A+L).After treatment for 6 weeks,dopamine(DA)neurons and the pathological changes of tyrosine hydroxylase(TH)positive cells were observed.The enzyme-linked immunosorbent assay(ELISA)was used to measure the content of DA and the level of interleukin-1β(IL-1β),interleukin-6(IL-6),interleukin-10(IL-10)and tumor necrosis factor alpha(TNF-α).The m RNA level of PINK1 and Parkin and the protein expression of Nix,PINK1 and Parkin in the substantia nigra were also detected.RESULTS:Combination treatment effectively ameliorated the symptoms of PD.Compared with model group,combined treatment significantly up-regulated the protein expression of Nix,Parkin and PINK1 and the m RNA levels of PINK1 and Parkin in the substantia nigra(P<0.0001,P<0.001,P<0.01 or P<0.05).Furthermore,the levels of pro-inflammation cytokines were obviously decreased after combination therapy,while IL-10 content was increased remarkably(P<0.01).CONCLUSION:Compared with each treatment alone,combination therapy improved the pathological damage of DA neurons of PD mice more effectively.The possible mechanism may be attributed to the up-regulated level of mitochondrial autophagy and improved mitochondrial function.These results provide fresh insight into the mechanism of co-treatment with Tongdu Tiaoshen acupuncture and XXMD for PD.
基金Supported by Research Program of Anhui University of Traditional Chinese Medicine:2019zryb21。
文摘Objective:To observe the impacts of the combined treatment of Tongdu Tiaoshen moxibustion(moxibustion for promoting the circulation of the governor vessel and regulating the spirit)and rehabilitation training on the motor function recovery in the patients with apoplectic hemiplegia.Methods:A total of 50 patients with apoplectic hemiplegia and qualified in the trial recruitment criteria were divided into two groups according to random number table,25 cases in each group.In the control group,the simple rehabilitation training was provided.In the observation group,on the base of the treatment as the control group,Tongdu Tiaoshen moxibustion was given.Main acupoints:Baihul(百会GV20),Fengfu(风府GV16)and Dazhui(大椎GV14).The treatment was given once a day,6 treatments a week,4 weeks as one course and two courses of treatment were required.Before and after treatment,the scores of Fugle-Meyer assessment(FMA),the modified Barthel index(MBI)and action research arm test(ARAT)were detected before and after treatment in the two groups separately.Results:After 4 and 8 weeks of treatment,the scores of FMA,MBI and ARAT were all improved obviously as compared with those before treatment respectively in patients of the two groups(all P<0.01).After8 weeks of treatment,the score of each scale in the observation group was more obviously improved as compared with the control group(all P<0.05).The difference in the clinical therapeutic effect was significant statistically between the two groups(P=0.005).Conclusion:The combined treatment of Tongdu Tiaoshen moxibustion and rehabilitation training promotes the recovery of the upper limb motor function and improves the self-ability of living activities in the patients with apoplectic hemiplegia.The long-term effect of this combined therapy is superior to that of simple rehabilitation training.
基金financially supported by the National Science Foundation of China(No.32271440).
文摘Lumbar spinal stenosis is caused by the compression of the nerve root or cauda equina nerve by stenosis of the lumbar spinal canal or intervertebral foramen,and is manifested as chronic low back and leg pain.Danlu Tongdu(DLTD)tablets can relieve chronic pain caused by lumbar spinal stenosis,but the molecular mechanism remains largely unknown.In this study,the potential molecular mechanism of DLTD tablets in the treatment of lumbar spinal stenosis was first predicted by the network pharmacology method.Results showed that DLTD functions in regulating anti-oxidative,apoptosis,and inflammation signaling pathways.Furthermore,the flow cytometry results showed that DLTD tablets efficiently reduced reactive oxygen species content and inhibited rat neural stem cell apoptosis induced by hydrogen peroxide.DLTD also inhibited the mitochondrial membrane potential damage induced by hydrogen peroxide.Elisa analysis showed that DLTD induced cell cycle-related protein,CDK2 and CDK4,and reduced CDKN1A protein expression level.Taken together,our study provided new insights of DLTD in treating lumbar spinal stenosis through reducing reactive oxygen species content,decreasing apoptosis by inhibiting CDKN1A and promoting CDK2 and CDK4 expression levels.
基金Research Project of Health Commission of Hubei Province(WJ2017F072):Study on Brain Function Imaging Mechanism of Scalp Acupuncture Treatment of Post-Stroke Depression.
文摘[Objectives]To evaluate the effects of Tongdu Tiaoshen Acupuncture method on improving learning and memory function and hippocampal nerve regeneration and repair after stroke.[Methods]Animal models of cognitive impairment in the recovery phase of cerebral infarction in rats were prepared,and rats were randomly divided into control group,model group,donepezil group,and acupuncture group.Morris water maze experiment was carried out to evaluate learning and memory ability.Nissl staining,BrdU and DCX immunofluorescence staining were used to observe the number and morphological structure of neuronal pyramidal and granular cells in the dentate gyrus(DG)area of the hippocampus,as well as the proliferation and differentiation of neural progenitor cells.TUNEL method was used to detect cell apoptosis.[Results]Before intervention,the scores and ELP of the model group,donepezil group and acupuncture group were significantly higher than those of the blank group(P<0.05);after the intervention,the neurological scores and ELP of the blank group and the model group were not significantly different from those before the intervention(P<0.05);the donepezil group and acupuncture group were significantly reduced,there was no difference between the donepezil group and acupuncture group(P>0.05),but it was still significantly higher than the blank group(P<0.05).The results of BrdU and DCX immunofluorescence staining showed that there were only a few BrdU positive cells and DCX protein in the hippocampal DG area of mice in the blank group,and the positive cells in the model group were significantly increased(P<0.05);donepezil group and acupuncture group showed significant increase compared with the model group(P<0.05);compared with the blank group,the number of neurons in the hippocampal DG area of each group was significantly reduced(P<0.01),and the neuronal apoptosis index was significantly increased(P<0.01);compared with the blank group,the number of neurons in the hippocampal DG area of each group was significantly reduced(P<0.01),and the neuronal apoptosis index was significantly increased(P<0.01).The number of neurons in the hippocampal DG area of rats in the acupuncture group and donepezil group was significantly increased(P<0.01),and the neuronal apoptosis index was significantly decreased(P<0.01).[Conclusions]Tongdu Tiaoshen Acupuncture method can improve the learning and memory function of stroke model rats by promoting the regeneration and repair of hippocampal nerve in rats with cerebral infarction.
文摘Spinal disorders are diseases caused by congenital, degenerative, postural errors, trauma, and external evils that result in lesions of bones, discs, ligaments, and muscles, thus compressing the nerves. Spine-related diseases include scoliosis, intervertebral disc herniation, intervertebral stenosis, spinal fracture, ankylosing spondylitis, spinal cord injury, spinal tumour, etc., which seriously affect people’s daily life. This paper summarises the relevant studies on the treatment of spinal diseases by Tongzhu therapy. The mechanism of action of Tongzhu therapy in treating spinal diseases is closely related to the control of inflammatory factors, the improvement of local microcirculation, analgesia, and the promotion of nerve repair. This paper describes the application and modern mechanism of Tongzhu therapy in the treatment of spinal diseases, which will provide a direction for future research on Tongzhu therapy.
文摘Objective To observe the effects of Tongdu Tiaoshen acupuncture(for unblocking the obstruction in the governor vessel and regulating the spirit)on depressionlike behavior and the hippocampal Endophilin A1/reactive oxygen species(ROS)pathway in the chronic unpredictable mild stress(CUMS)model rats,and explore the mechanism of this therapy for depression.Methods Forty-eight male SD rats of SPF grade were randomly divided into a normal group(n=12) and a modeling group(n=36).
文摘Objective To explore the metabolomic changes and possible mechanism of Tongdu Xingshen electroacupuncture in alleviating middle cerebral artry occlusion/reperfusion(MCAO/R) learning and memory impairment rats.Methods A total of 90 SD rats were divided into a control group(n=15),a sham group(n=15),and 60 MCAOR rat models were established by thread suppositing method.
文摘Objective To study the action mechanism of Wenshen Tongdu Recipe(WSTDR)inpromoting microvascular endothelial cells(MECs)phagocytosis and clearing myelin debris by reducing iron overload phenomenon after spinal cord injury,thereby improving the infiammatory microenvironment after spinal cord injury.Methods Fifty C57BL/6 mice were divided into 5 groups by random number table method:sham operation group,model group,deferoxamine(DFO)group,WSTDR group,WSTDR+DFO group,10 mice in each group.A mouse model of spinal cord injury was prepared by modified Allen's method.The pathological changes of the spinal cord were observed by HE staining and Nissl staining.The iron ion content in serum was determined by the iron ion colorimetric assay kit.The expression levels of monocyte chemoattractant protein-1(MCP-1),interleukin(IL)-6,IL-1β,and tumor necrosis factor-α(TNF-α)in the homogenate and serum of the spinal cord were detected by ELISA.The phagocytosis and clearance of myelin debris by MECs in the spinal cord were observed by 3D laser confocal.Results One mouse died in the model group,DFO group,and WSTDR group,respectively.The results of HE staining showed that compared with the model group,spinal cord cavities were reduced with nerve fiber disorder in DFO group and WSTDR group.Nissl staining analysis showed that compared with the model group,Nissl morphology and neuronal pyknosis were improved in DFO group and WSTDR group.Ferric ion colorimetric assay showed that compared with the model group,serum iron ion concentration were significantly reduced in DFO group and WSTDR group(P<0.01).ELISA results showed that compared with the sham-operation group,the levels of MCP-1,IL-6,IL-1β,and TNF-αin serum and spinal cord tissues significantly increased in the model group(P<0.01).Compared with the model group,serum levels of MCP-1,IL-6,IL-1β,and TNF-αwere significantly lowered in DFO group and WSTDR group(P<0.05,P<0.01).Serum concentrations of MCP-1 and IL-1βwere significantly lowered in WSTDR+DFO group(P<0.01).Compared with WSTDR group,serum concentrations of MCP-1,IL-6,anddIL-1βwere significantly improved in WSTDR+DFO group(P<0.01).Compared with the model group,the levels of MCP-1,IL-6,IL-1β(except WSTDR group),and TNF-αin spinal cord tissues were significantly reduced in DFO group,WSTDR group,and WSTDR+DFO group(P<0.05,P<0.01).3D laser confocal results showed MECs phagocytosis and clearance of myelin debris were promoted in DFO group and WSTDR group.Conclusion WSTDR promotes MECs to engulf myelin debris by reducing iron overload,improving the infiammatory microenvironment,and promoting spinal cord injury repairment.
