Sechium edule(chayote)is an important vegetable crop belonging to the Cucurbitaceae family.To decipher the chayote genome,a highquality chromosome-level chayote genome was obtained by genome sequencing and bioinformat...Sechium edule(chayote)is an important vegetable crop belonging to the Cucurbitaceae family.To decipher the chayote genome,a highquality chromosome-level chayote genome was obtained by genome sequencing and bioinformatic analysis.The total length was612.91 Mb,and 25755 genes were detected in the chayote genome.The contig N50 was more than 20.01 Mb,and the scaffold N50 was over47.11 Mb.Of the genome,60.35%were composed of repetitive sequences,and 31.18%of genome sequences belonged to long-terminal repeats.A global alignment of homologous regions in chayote and other Cucurbitaceae plant genomes was constructed using grape as a reference.Based on this genome-wide and global alignment map,researchers can easily identify homologous collinear genes of the studied genomes in most Cucurbitaceae species.Twenty-five chayote accessions were divided into two subgroups based on phylogenetic tree,population structure analysis,and principal component analysis using genome re-sequencing data.The chayote genome,re-sequencing dataset,and comprehensive genomic analysis will accelerate comparative and functional genomic analysis of chayote and other Cucurbitaceae species in the future.展开更多
本文以佛手瓜为原料,采用响应面法优化超声辅助提取佛手瓜多糖。在单因素试验基础上,运用Box-Behnken中心组合实验和响应面法考察了液料比、超声功率、超声温度、超声时间四个因素对佛手瓜多糖得率的影响,并优化了提取工艺。结果表明最...本文以佛手瓜为原料,采用响应面法优化超声辅助提取佛手瓜多糖。在单因素试验基础上,运用Box-Behnken中心组合实验和响应面法考察了液料比、超声功率、超声温度、超声时间四个因素对佛手瓜多糖得率的影响,并优化了提取工艺。结果表明最佳的工艺条件为:液料比30 m L·g^(-1)、超声功率245 W、超声温度65℃,超声40 min。在此条件下佛手瓜多糖得率的预测值为3.319%,验证实验值为3.288%,其相对误差为0.934%。展开更多
基金supported by the National Natural Science Foundation of China Project(Grant No.32260097)the National Guidance Foundation for Local Science and Technology Development of China(Grant No.[2023]009)the Natural Science Foundation for Distinguished Young Scholars of Hebei(Grant No.C2022209010)。
文摘Sechium edule(chayote)is an important vegetable crop belonging to the Cucurbitaceae family.To decipher the chayote genome,a highquality chromosome-level chayote genome was obtained by genome sequencing and bioinformatic analysis.The total length was612.91 Mb,and 25755 genes were detected in the chayote genome.The contig N50 was more than 20.01 Mb,and the scaffold N50 was over47.11 Mb.Of the genome,60.35%were composed of repetitive sequences,and 31.18%of genome sequences belonged to long-terminal repeats.A global alignment of homologous regions in chayote and other Cucurbitaceae plant genomes was constructed using grape as a reference.Based on this genome-wide and global alignment map,researchers can easily identify homologous collinear genes of the studied genomes in most Cucurbitaceae species.Twenty-five chayote accessions were divided into two subgroups based on phylogenetic tree,population structure analysis,and principal component analysis using genome re-sequencing data.The chayote genome,re-sequencing dataset,and comprehensive genomic analysis will accelerate comparative and functional genomic analysis of chayote and other Cucurbitaceae species in the future.
文摘本文以佛手瓜为原料,采用响应面法优化超声辅助提取佛手瓜多糖。在单因素试验基础上,运用Box-Behnken中心组合实验和响应面法考察了液料比、超声功率、超声温度、超声时间四个因素对佛手瓜多糖得率的影响,并优化了提取工艺。结果表明最佳的工艺条件为:液料比30 m L·g^(-1)、超声功率245 W、超声温度65℃,超声40 min。在此条件下佛手瓜多糖得率的预测值为3.319%,验证实验值为3.288%,其相对误差为0.934%。
