摘要
对佛手瓜下胚轴再生植株、试管苗移栽及大田定植进行了试验，结果表明：在ＭＳ＋ＩＡＡ０．１ｍｇ／Ｌ＋ＢＡ１．０ｍｇ／Ｌ＋ＬＨ２００ｍｇ／Ｌ培养基上容易形成下胚轴愈伤组织；愈伤组织分化芽的培养基为ＭＳ＋ＩＡＡ０．５ｍｇ／Ｌ＋ＢＡ０．５ｍｇ／Ｌ，诱导分化率可达５７５％；顶芽和带腋芽茎段生根的培养基分别为ＭＳ＋ＩＡＡ１．０ｍｇ／Ｌ和１２ＭＳ＋ＩＡＡ０．３ｍｇ／Ｌ，生根率分别为８１．８％和８３．３％；移栽培养土用土砂粪＝３２１的灭菌土，幼苗成活率达７５％；大田定值，平均单株结果８８．４３个，平均单果重２４０．２３ｇ．
Plantlet regeneration from hypocotyl of Buddhas hand ( Sechium edule Swortz ),plantlet transfering and its fruiting in the field were studied.THe results showed that the calli were apt to be induced from hypocotyls at MS meddium supplemented with 0.1 mg/L IAA.1.0 mg/L BA and 200 mg/L LH;Shoots were easily differentiated from tissue calli at MS mediu with 0.5 mg/L IAA and 0.5 mg/L BA,with the differentiation ratio of 575%;The optimal rooting medium for terminal bud and stem segments with axillary buds were MS medium containing 1.0 mg/L IAA and 1/2 MS medium containing 0.3 mg/L IAA respectively.Their rooting ratio was 81.8% and 83.3% respectively.The conposts composed of 3 parts of soil,2 parts of sand and 1 part of mannul were sterilized before transplanting.The survival rate of transferred plantlets reached 75%.THe average number of fruits per plant was 88.43 and the average weight per fruit was 240.2 g.
出处
《西北农业大学学报》
CSCD
北大核心
1997年第1期83-87,共5页
Journal of Northwest Sci-Tech University of Agriculture and Forestry（Natural Science Edition)
关键词
佛手瓜
下胚轴
离体培养
再生植株
繁殖系数
Buddhas hand ( Sechium edule Swortz ),hypocotyl, In Vitro cultue,regenerated plantlet,propagation,coefficient
