[Objective] This study was to develop an in vitro tissue culture system of Rosa spp.[Method] Using ten species of Rosa spp.plants as experimental materials,different combinations of hormones were designed to establish...[Objective] This study was to develop an in vitro tissue culture system of Rosa spp.[Method] Using ten species of Rosa spp.plants as experimental materials,different combinations of hormones were designed to establish their in vitro tissue culture system with the stem segments as explants.[Result] All ten tested varieties germinated when the nodal segment explants were cultured on the sprouting medium MS＋ 0.5 mg/L BA ＋0.01 mg/L NAA and grew vigorous shoots,and the sprouting rate was up to 70%.Of the ten tested rose varieties,each has a respective optimal proliferation medium,and the multiplication rates for all the varieties reached 3.0%.The axillary buds were vigorous and normal in leaf color.The optimal medium for rooting and acclimation was 1/2MS medium containing 0.1 mg/L or 0.2 mg/L NAA,in which the rooting frequency reached 90%-100% and the root system was developed.After acclimation and transplant,the survival rate was as high as 95%.[Conclusion] An in vitro tissue culture system of Rosa spp.has been established in this study,which lays foundation for the molecular breeding of Rosa spp.展开更多
月季(Rosa spp.)是一种具有重要价值的观赏植物,花色是其最重要的观赏性状之一。为了解析月季花色形成的分子机理,本研究以‘云蒸霞蔚’和‘太阳城’的杂交后代为试验材料,探索类黄酮和类胡萝卜素的提取与测定方法,并且对‘梅朗口红’...月季(Rosa spp.)是一种具有重要价值的观赏植物,花色是其最重要的观赏性状之一。为了解析月季花色形成的分子机理,本研究以‘云蒸霞蔚’和‘太阳城’的杂交后代为试验材料,探索类黄酮和类胡萝卜素的提取与测定方法,并且对‘梅朗口红’花瓣中类黄酮和类胡萝卜素进行定性定量分析。结果表明:(1)类黄酮提取的最佳浸提剂为甲醇-水-甲酸-三氟乙酸(70∶27∶2∶1, V/V),采用超声波辅助提取,流动相为0.5%甲酸水溶液(A)和乙腈(B),洗脱梯度为0 min 5%B,5 min 10%B,30 min 19%B,50 min 40%B,50.01~60 min 5%B;(2)类胡萝卜素提取的最佳浸提剂为甲醇(含0.1%BHT),需要皂化,最佳复溶溶剂为甲醇-甲基叔丁基醚(1∶1,V/V,含0.1%BHT);(3)‘梅朗口红’花瓣中总共检测到1种花青苷、8种黄酮醇和6种类胡萝卜素,其中花青苷含量最高,黄酮醇次之,类胡萝卜素最低,开花过程中色素含量变化显著,矢车菊3,5-双氧葡萄糖苷的含量先升高后降低,在S3时期,最大值为1 705.90μg/g FW,槲皮素3-O-鼠李糖苷、槲皮素总含量以及黄酮醇总含量先升高后降低,在S2时期达到最大值,槲皮素7-O-鼠李糖苷和山奈酚总含量逐渐下降;(4)相关分析显示,明度L*与花青苷含量极显著负相关,色相a*与花青苷含量显著正相关。本研究不仅为月季花色的分子育种提供帮助,同时也为月季花瓣中功能物质的开发提供借鉴。展开更多
基金Supported by National Natural Science Foundation of China(30871733)~~
文摘[Objective] This study was to develop an in vitro tissue culture system of Rosa spp.[Method] Using ten species of Rosa spp.plants as experimental materials,different combinations of hormones were designed to establish their in vitro tissue culture system with the stem segments as explants.[Result] All ten tested varieties germinated when the nodal segment explants were cultured on the sprouting medium MS＋ 0.5 mg/L BA ＋0.01 mg/L NAA and grew vigorous shoots,and the sprouting rate was up to 70%.Of the ten tested rose varieties,each has a respective optimal proliferation medium,and the multiplication rates for all the varieties reached 3.0%.The axillary buds were vigorous and normal in leaf color.The optimal medium for rooting and acclimation was 1/2MS medium containing 0.1 mg/L or 0.2 mg/L NAA,in which the rooting frequency reached 90%-100% and the root system was developed.After acclimation and transplant,the survival rate was as high as 95%.[Conclusion] An in vitro tissue culture system of Rosa spp.has been established in this study,which lays foundation for the molecular breeding of Rosa spp.
文摘月季(Rosa spp.)是一种具有重要价值的观赏植物,花色是其最重要的观赏性状之一。为了解析月季花色形成的分子机理,本研究以‘云蒸霞蔚’和‘太阳城’的杂交后代为试验材料,探索类黄酮和类胡萝卜素的提取与测定方法,并且对‘梅朗口红’花瓣中类黄酮和类胡萝卜素进行定性定量分析。结果表明:(1)类黄酮提取的最佳浸提剂为甲醇-水-甲酸-三氟乙酸(70∶27∶2∶1, V/V),采用超声波辅助提取,流动相为0.5%甲酸水溶液(A)和乙腈(B),洗脱梯度为0 min 5%B,5 min 10%B,30 min 19%B,50 min 40%B,50.01~60 min 5%B;(2)类胡萝卜素提取的最佳浸提剂为甲醇(含0.1%BHT),需要皂化,最佳复溶溶剂为甲醇-甲基叔丁基醚(1∶1,V/V,含0.1%BHT);(3)‘梅朗口红’花瓣中总共检测到1种花青苷、8种黄酮醇和6种类胡萝卜素,其中花青苷含量最高,黄酮醇次之,类胡萝卜素最低,开花过程中色素含量变化显著,矢车菊3,5-双氧葡萄糖苷的含量先升高后降低,在S3时期,最大值为1 705.90μg/g FW,槲皮素3-O-鼠李糖苷、槲皮素总含量以及黄酮醇总含量先升高后降低,在S2时期达到最大值,槲皮素7-O-鼠李糖苷和山奈酚总含量逐渐下降;(4)相关分析显示,明度L*与花青苷含量极显著负相关,色相a*与花青苷含量显著正相关。本研究不仅为月季花色的分子育种提供帮助,同时也为月季花瓣中功能物质的开发提供借鉴。
文摘【目的】通过研究月季新叶与花色的相关性,探讨月季生产早期定向选择的方法,解决月季育种周期长、育种效率低等问题。【方法】以月季"云蒸霞蔚"×"太阳城"的F1代植株为材料,测定了其新叶相对叶绿素含量(soil and plant analyzer development,SPAD)、花青苷含量、色差值及花瓣色差值,用CIELab系统对叶色和花色进行描述,探索新叶各参数与花瓣色差值之间的关联性。【结果】花瓣亮度L^(*)和色相a^(*)、饱和度C呈显著负相关,根据L^(*)与C的关系,可将F1代植株分为2个类群;新叶花青苷含量与花瓣L^(*)呈显著负相关,与a^(*)和C呈显著正相关;新叶L^(*)和花瓣L^(*)、新叶a^(*)和花瓣a^(*)之间均呈显著正相关,新叶a^(*)与花瓣L^(*)、新叶L^(*)与花瓣a^(*)、新叶C与花瓣C间呈显著负相关。【结论】利用月季新叶花青苷含量和色差值对其花色进行预选具有可行性。
