摘要
花青素苷是玫瑰(Rosa spp.)花瓣呈色的主导色素,对其色泽表现具有决定性作用。本研究基于靶向代谢组学分析花瓣呈色由浅至深的‘紫枝玫瑰’(R.rugosa‘Zizhi Meigui’)、‘丰花玫瑰’(R.rugosa‘Fenghua Meigui’)、大马士革玫瑰(R.damascena)、苦水玫瑰(R.sertata×R.rugosa)4种玫瑰花瓣中花青素苷的组成,旨在阐明花青素苷含量与玫瑰花瓣呈色的相关性。通过超高效液相色谱串联质谱(UPLC-MS/MS)检测与分析,揭示了玫瑰花瓣中花青素苷衍生物构成的核心组分为矢车菊素-3,5-O-二葡萄糖苷和芍药花素-3,5-O-二葡萄糖苷,两种组分共占花青素苷总量的73.77%~81.57%。此外,还检测到少量天竺葵素、飞燕草素和极微量矮牵牛素、锦葵色素。随着玫瑰花器官的生长发育,花瓣中花青素苷含量会逐渐降低。相关性分析发现芍药花素-3,5-O-二葡萄糖苷的积累对于玫瑰花瓣展现红色调具有积极影响,飞燕草素-3,5-O-二葡萄糖苷的积累会促使玫瑰花瓣亮度值降低。结果表明,矢车菊素-3,5-O-二葡萄糖苷和芍药花素-3,5-O-二葡萄糖苷共同影响玫瑰花瓣的着色程度,飞燕草素-3,5-O-二葡萄糖苷则调控花瓣的亮度。本研究深入剖析了玫瑰花瓣呈色的主导花青素苷,揭示了玫瑰花中花青素苷的主要代谢途径,为更多色系的玫瑰新品种选育提供理论支撑。
Anthocyanins serve as the dominant pigments in rose(Rosa spp.)petals,playing a decisive role in their coloration.This study employed targeted metabolomics to analyze the anthocyanin composition in the petals of four rose species-R.rugosa'Zizhi Meigui',R.rugosa'Fenghua Meigui',R.damascena,and R.sertata×R.rugosa-displaying a gradient of color intensity from light to dark.The aim was to elucidate the correlation between anthocyanin content and petals coloration.Using UPLC-MS/MS,the main coloring agents identified in rose petals were peonidin-3,5-O-diglucoside and cyanidin-3,5-O-diglucoside,which accounted for 73.77%to 81.57%of the total anthocyanins.Minor amounts of pelargonidin and delphinidin,along with trace quantities of petunidin and malvidin,were also detected.With the growth and development of rose flowers,the anthocyanins in the petals gradually degrade.Correlation analysis revealed that the accumulation of peonidin-3,5-O-diglucoside was more conducive to red coloration in rose petals,whereas the accumulation of delphinidin-3,5-O-diglucoside reduced the brightness value of the petals.The results indicated that cyanidin-3,5-O-diglucoside and peonidin-3,5-O-diglucoside jointly influenced the degree of petal coloration,while delphinidin-3,5-O-diglucoside regulated petal brightness.This study analyzed the primary anthocyanins affecting rose petal coloration,revealed the main metabolic pathways of anthocyanins in roses,and provided a theoretical basis for the breeding of new rose varieties with a broader color spectrum.
作者
杨阳
李娇娇
李军
郭爱华
YANG Yang;LI Jiaojiao;LI Jun;GUO Aihua(Department of Biological and Food Engineering,Lyuliang University,Liliang,Shanxi 033000,China;Luliang Key Laboratory of Horticultural Plants Germplasm Resources Innovation and Genetic Improvement,Luliang,Shanxi033000,China;Characteristic Plant Resource Chemistry Provincial and Municipal Joint Construction Shanxi Provincial Key Laboratory Cultivation Base,Luliang,Shanxi 033000,China)
出处
《植物生理学报》
北大核心
2025年第6期749-764,共16页
Plant Physiology Journal
基金
山西省基础研究计划(自由探索类)青年科学研究项目(202103021223384)
吕梁市引进高层次科技人才重点研发项目(2022RC20和2023RC12)
吕梁市平台基地建设项目(2022ZDSY02)
吕梁市重点研发计划项目(农业领域)(2024NY10)。
