BACKGROUND Diabetes is a metabolic disease with a high complication rate.Diabetic foot ulcers(DFUs)seriously affect the quality of life of patients.A total of 15%-20%of diabetic patients develop DFUs,which heal with d...BACKGROUND Diabetes is a metabolic disease with a high complication rate.Diabetic foot ulcers(DFUs)seriously affect the quality of life of patients.A total of 15%-20%of diabetic patients develop DFUs,which heal with difficulty over a long time and can result in amputation and disability.Traditional Chinese medicine has a unique effect in the treatment of skin ulcerative diseases.Ruyi Jinhuang powder(RHP)is one of the classic prescriptions in traditional Chinese medicine and is widely used in clinical practice.AIM To verify the ability of RHP to promote wound healing by electron microscopy analysis in animal models and hematoxylin-eosin(HE)staining.The effective components of RHP were extracted and identified by gas chromatography-mass spectrometry(GC-MS),and the obtained chemical components were analyzed by network pharmacology methods to predict its therapeutic mechanism.METHODS Sprague Dawley rats were injected with streptozotocin to establish the DFU model.HE staining was used to observe the wound tissue under an electron microscope.The chemical constituents of RHP were extracted first by supercritical fluid extraction and alcohol extraction,and then,GC-MS and ultra-performance liquid chromatography–MS were used to separately identify the chemical constituents.In addition,the"herb-component-target"link was established through the Traditional Chinese Medicine Systems Pharmacology database to obtain the target information,and the molecular docking of important components and key targets was performed in Discovery Studio software.Cytoscape software was used to visualize and analyze the relationship between the chemical composition,targets and Traditional Chinese Medicine network.RESULTS RHP promoted DFU healing in rats by affecting fibroblasts and nerve cells.A total of 89 chemical components were obtained by GC-MS.Network pharmacological analysis revealed that RHP was associated with 36 targets and 27 pathways in the treatment of DFU,of which the important components were luteolin,trans caryophyllene,ar-turmerone,palmitic acid,methyl palmitate,gallic acid,demethoxycurcumin,berberine,and rheic acid.The key targets were posttranscriptional silencing,topoisomerase II alpha,muscarinic acetylcholine receptor M2,interleukin 6,tumor necrosis factor and retinoic X receptor alpha,and the key pathways were the phosphoinositide 3-kinase-protein kinase B signaling pathway,neuroactive ligand–receptor interactions,and the forkhead box O signaling pathway.CONCLUSION Our results indicated that RHP may play a role in the treatment of DFU through these target pathways by affecting insulin resistance,altering the nervous system and immune system,participating in inflammatory responses and regulating cell proliferation,differentiation and apoptosis through other specific mechanisms.展开更多
Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-...Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-blind,placebo-controlled trial encompassing a three-menstrual cycle intervention followed by a three-menstrual cycle follow-up period.A total of 164 eligible females with endometriosis-associated dysmenorrhea were randomly divided into HHRY pills and placebo groups in a 1:1 ratio.The primary outcome included dysmenorrhea symptoms assessed using Visual Analog Scale(VAS)scores and quality of life,whereas the secondary outcome measures included the maximum VAS for non-menstrual pelvic pain,duration of pain episodes(in days),frequency and quantity of the consumption of ibuprofen sustained-release capsules(or other non-steroidal anti-inflammatory drugs),and days off work/study for staff/student due to dysmenorrhea,ovarian cyst,and/or pelvic nodule size.The safety was monitored throughout the treatment period.All the analyses were based on the intention-to-treat principle.For continuous outcomes,simple or multiple linear regressions were used to estimate the differences between the HHRY pills and placebo groups,with categorical data expressed as the number and percentage of occurrences.Differences were compared using the chi-square test or Fisher's exact test.The predefined analysis was adjusted for concomitant treatment,a variable considered to be associated with outcomes but unaffected by treatment allocation.Estimates of treatment effects were reported with 95%confidence intervals.Two-tailed P values≤.05 were considered statistically significant.Conclusion:Positive results from this trial,upon completion would provide robust evidence for the efficacy and safety of HHRY pills in treating dysmenorrhea in patients with endometriosis.展开更多
Objective:To evaluate the efficacy and safety of Tibetan medicine Ruyi Zhenbao Pills(RZPs)in the treatment of patients with motor and sensory dysfunction after stroke.Methods:A total of 120 convalescent stroke patient...Objective:To evaluate the efficacy and safety of Tibetan medicine Ruyi Zhenbao Pills(RZPs)in the treatment of patients with motor and sensory dysfunction after stroke.Methods:A total of 120 convalescent stroke patients hospitalized in the Rehabilitation Department of Guangdong Provincial Hospital of Chinese Medicine from June 2017 to December 2019 were enrolled in this trial.Patients were assigned to control(60 cases)and research(60 cases)groups by computer random assignment.All patients received internal treatment and modern rehabilitation training.On this basis,the research group was given oral RZPs for 4 weeks,while the control group was given oral placebo.The primary outcome was motor function of the affected side evaluated by simplified FuglMeyer Motion Assessment Scale(FMA-M).The secondary outcomes included sensory function,activity of daily living(ADL),quality of life,balance function,and pain,which were assessed by Fugl-Meyer Sensory Assessment Scale(FMA-S),Modified Barthel Index(MBI),Special Scale of the Quality of Life(SS-QOL),Berg Balance Scale(BBS),and Visual Analogue Scale(VAS),respectively.All of the assessments were performed before treatment,and 4 and 8 weeks after treatment.Vital signs,liver and kidney functions,routine blood test,blood coagulation profile,and routine urinalysis of patients were monitored.Results:After 4-week treatment,the FMA-M,BBS and FMA-S scores in the research group significantly increased compared with the control group(P<0.05).At 8-week follow-up,the BBS and MBI scores in the research group were higher than the control group(P<0.05).There was no statistical difference between the 2 groups in the SS-QOL and VAS scores at 4 and 8 weeks(P>0.05).Moreover,after treatment,there was no significant difference in vital signs,liver and kidney functions,blood coagulation function,blood routine and urinalysis between the 2 groups(P>0.05).Conclusion:RZPs improved limb motor,balance,and sensory functions of stroke patients during recovery period with good safety.(Trial registration No.NCT04029701)展开更多
The list of 2008 Top 1O Dyeing&Printing Companieswas unveiled in Beijing recently.Shandong Ruyi was awardedthis title again by China Dyeing&Printing Association.Under the impact of global financial crisis,Ruyi...The list of 2008 Top 1O Dyeing&Printing Companieswas unveiled in Beijing recently.Shandong Ruyi was awardedthis title again by China Dyeing&Printing Association.Under the impact of global financial crisis,Ruyi madegreat effort to improve quality and reduce the cost,and establishits brand name in African market.It is reported thatVIVI WEX brand dyeing fabric of this company is popular展开更多
基金Supported by National Natural Science Foundation of China,No.82074025Scientific Research Project of Heilongjiang Health Committee,No.2020-293Scientific and Technological Innovation Project for College Students of Heilongjiang University of Chinese Medicine,No.2021-13.
文摘BACKGROUND Diabetes is a metabolic disease with a high complication rate.Diabetic foot ulcers(DFUs)seriously affect the quality of life of patients.A total of 15%-20%of diabetic patients develop DFUs,which heal with difficulty over a long time and can result in amputation and disability.Traditional Chinese medicine has a unique effect in the treatment of skin ulcerative diseases.Ruyi Jinhuang powder(RHP)is one of the classic prescriptions in traditional Chinese medicine and is widely used in clinical practice.AIM To verify the ability of RHP to promote wound healing by electron microscopy analysis in animal models and hematoxylin-eosin(HE)staining.The effective components of RHP were extracted and identified by gas chromatography-mass spectrometry(GC-MS),and the obtained chemical components were analyzed by network pharmacology methods to predict its therapeutic mechanism.METHODS Sprague Dawley rats were injected with streptozotocin to establish the DFU model.HE staining was used to observe the wound tissue under an electron microscope.The chemical constituents of RHP were extracted first by supercritical fluid extraction and alcohol extraction,and then,GC-MS and ultra-performance liquid chromatography–MS were used to separately identify the chemical constituents.In addition,the"herb-component-target"link was established through the Traditional Chinese Medicine Systems Pharmacology database to obtain the target information,and the molecular docking of important components and key targets was performed in Discovery Studio software.Cytoscape software was used to visualize and analyze the relationship between the chemical composition,targets and Traditional Chinese Medicine network.RESULTS RHP promoted DFU healing in rats by affecting fibroblasts and nerve cells.A total of 89 chemical components were obtained by GC-MS.Network pharmacological analysis revealed that RHP was associated with 36 targets and 27 pathways in the treatment of DFU,of which the important components were luteolin,trans caryophyllene,ar-turmerone,palmitic acid,methyl palmitate,gallic acid,demethoxycurcumin,berberine,and rheic acid.The key targets were posttranscriptional silencing,topoisomerase II alpha,muscarinic acetylcholine receptor M2,interleukin 6,tumor necrosis factor and retinoic X receptor alpha,and the key pathways were the phosphoinositide 3-kinase-protein kinase B signaling pathway,neuroactive ligand–receptor interactions,and the forkhead box O signaling pathway.CONCLUSION Our results indicated that RHP may play a role in the treatment of DFU through these target pathways by affecting insulin resistance,altering the nervous system and immune system,participating in inflammatory responses and regulating cell proliferation,differentiation and apoptosis through other specific mechanisms.
基金supported by the National Natural Science Foundation of China(81830115).
文摘Objective:To provide high-quality clinical evidence of the efficacy of Tibetan medicine Honghua Ruyi(HHRY)pills for endometriosis-associated dysmenorrhea.Methods:This study constitutes a multicenter,randomized,double-blind,placebo-controlled trial encompassing a three-menstrual cycle intervention followed by a three-menstrual cycle follow-up period.A total of 164 eligible females with endometriosis-associated dysmenorrhea were randomly divided into HHRY pills and placebo groups in a 1:1 ratio.The primary outcome included dysmenorrhea symptoms assessed using Visual Analog Scale(VAS)scores and quality of life,whereas the secondary outcome measures included the maximum VAS for non-menstrual pelvic pain,duration of pain episodes(in days),frequency and quantity of the consumption of ibuprofen sustained-release capsules(or other non-steroidal anti-inflammatory drugs),and days off work/study for staff/student due to dysmenorrhea,ovarian cyst,and/or pelvic nodule size.The safety was monitored throughout the treatment period.All the analyses were based on the intention-to-treat principle.For continuous outcomes,simple or multiple linear regressions were used to estimate the differences between the HHRY pills and placebo groups,with categorical data expressed as the number and percentage of occurrences.Differences were compared using the chi-square test or Fisher's exact test.The predefined analysis was adjusted for concomitant treatment,a variable considered to be associated with outcomes but unaffected by treatment allocation.Estimates of treatment effects were reported with 95%confidence intervals.Two-tailed P values≤.05 were considered statistically significant.Conclusion:Positive results from this trial,upon completion would provide robust evidence for the efficacy and safety of HHRY pills in treating dysmenorrhea in patients with endometriosis.
基金Supported by SHEN Bao-fan Academic Experience Inheritance Studio of Guangdong Provincial Hospital of Chinese Medicine。
文摘Objective:To evaluate the efficacy and safety of Tibetan medicine Ruyi Zhenbao Pills(RZPs)in the treatment of patients with motor and sensory dysfunction after stroke.Methods:A total of 120 convalescent stroke patients hospitalized in the Rehabilitation Department of Guangdong Provincial Hospital of Chinese Medicine from June 2017 to December 2019 were enrolled in this trial.Patients were assigned to control(60 cases)and research(60 cases)groups by computer random assignment.All patients received internal treatment and modern rehabilitation training.On this basis,the research group was given oral RZPs for 4 weeks,while the control group was given oral placebo.The primary outcome was motor function of the affected side evaluated by simplified FuglMeyer Motion Assessment Scale(FMA-M).The secondary outcomes included sensory function,activity of daily living(ADL),quality of life,balance function,and pain,which were assessed by Fugl-Meyer Sensory Assessment Scale(FMA-S),Modified Barthel Index(MBI),Special Scale of the Quality of Life(SS-QOL),Berg Balance Scale(BBS),and Visual Analogue Scale(VAS),respectively.All of the assessments were performed before treatment,and 4 and 8 weeks after treatment.Vital signs,liver and kidney functions,routine blood test,blood coagulation profile,and routine urinalysis of patients were monitored.Results:After 4-week treatment,the FMA-M,BBS and FMA-S scores in the research group significantly increased compared with the control group(P<0.05).At 8-week follow-up,the BBS and MBI scores in the research group were higher than the control group(P<0.05).There was no statistical difference between the 2 groups in the SS-QOL and VAS scores at 4 and 8 weeks(P>0.05).Moreover,after treatment,there was no significant difference in vital signs,liver and kidney functions,blood coagulation function,blood routine and urinalysis between the 2 groups(P>0.05).Conclusion:RZPs improved limb motor,balance,and sensory functions of stroke patients during recovery period with good safety.(Trial registration No.NCT04029701)
文摘The list of 2008 Top 1O Dyeing&Printing Companieswas unveiled in Beijing recently.Shandong Ruyi was awardedthis title again by China Dyeing&Printing Association.Under the impact of global financial crisis,Ruyi madegreat effort to improve quality and reduce the cost,and establishits brand name in African market.It is reported thatVIVI WEX brand dyeing fabric of this company is popular
