Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-...Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.展开更多
Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HP...Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.展开更多
Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysi...Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysis of spectroscopic data.All compounds were tested for their cytotoxic activity,and all compounds except 4 were tested for their acetylcholinesterase(AChE)inhibitory activities.展开更多
Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,...Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,and P3,through gradient elution using AB-8 macroporous resin columns.The primary constituents of these fractions include iridoids,phenylethanoid glucosides,and flavonoids.Subsequently,a comprehensive in vitro and in vivo screening and evaluation model was developed considering the fundamental pathological processes of gout,including the inhibition of uric acid(UA)production,augmentation of UA excretion,and anti-inflammatory effects.This model was used to analyze the anti-gout activity of P1,P2,P3,and the overall extracts derived from PAL.Results:All the PAL parts exhibited specific therapeutic properties as part of gout therapy.Specifically,the P2 part demonstrated the ability to diminish interleukin-1 beta levels,lower UA concentrations,suppress joint inflammation in rats,and restore abnormal parameters associated with gout,such as creatinine and urea nitrogen.These findings highlight the significant effect of P2 on gout treatment.Conclusions:Following a thorough assessment of the outcomes derived from diverse anti-gout experiments,P2 exhibited superior anti-gout properties compared to the remaining parts.P2 demonstrated a restorative effect on the aberrant parameters associated with gout,thereby comprehensively elucidating the therapeutic mechanism of P2 against gout.This study provides substantial evidence and serves as a reference for future investigations regarding the material foundation of PAL in gout treatment.展开更多
Box dimension and information dimension based on individual and leaf number were applied to analyze the distribution patterns of Plantato asiatica and P. viriginica in suburbs of Jinhua. The results showed that both t...Box dimension and information dimension based on individual and leaf number were applied to analyze the distribution patterns of Plantato asiatica and P. viriginica in suburbs of Jinhua. The results showed that both the box dimension and the information dimention of P. virginica were obviously higher than those of P. asiatica in the high human-distributed environment. P. asiatica was mostly distributed in the environment with compacted soil. The information dimension on the basis of leaf probability in the boxes showed that the populations of P. virginica and P. asiatica in low density could exploit environmental resources effectively.展开更多
Objective To identify the active compounds for protein tyrosine phosphatase 1B(PTP1B) from the seeds of Plantago asiatica.Methods Bioassay-guided fractionation resulted in the isolation of iridoid glucosides(1-5) with...Objective To identify the active compounds for protein tyrosine phosphatase 1B(PTP1B) from the seeds of Plantago asiatica.Methods Bioassay-guided fractionation resulted in the isolation of iridoid glucosides(1-5) with PTP1B inhibitory activity.Results Five compounds were identified as desacetylhookerioside(1),melittoside(2),geniposidic acid(3),10-O-acetyl-geniposidic acid(4),and alpinoside(5).Conclusion Isolated compounds 35 inhibit PTP1B with IC50 values ranged from(16.3 ± 1.1) to(19.8 ± 1.2) μmol/L.展开更多
基金supported by funding from Jilin Province (No.20060904-1 and 20071102).
文摘Two phenylethanoid glycosides （PhGs） were isolated and purified from the aerial parts of Plantago asiatica for the first time by high performance centrifugal partition chromatography （HPCPC） using ethyl acetate-n-butanol-ethanol-water （0.5：0.5：0.1：1, v/v/v/v）. A total of 45.6 mg of compound 1 and 293.8 mg of compound 2 were purified from 1341 mg of the n-butanol extract of P. asiatica. The structures of the two PhGs were tentatively identified as plantamajoside and aeteoside or isoacteoside by eleetrospray ionization multi stage tandem mass spectrometry （ESI-MS^n） in the negative ion mode.
基金Supported by the National Natural Science Foundation of China(No.30873364)the Natural Science Foundation of Jilin Province of China(Nos.20071102 and 2008-167)
文摘Two phenylethanoid glycosides（PhGs）, plantamajoside and acteoside, were isolated and purified from the aerial parts ofPlantago asiatica for the first time by high performance centrifugal partition chromatography（HPCPC） with ethyl acetate, n-butanol, ethanol and water（0.5：0.5：0.1：1, volume ratio） as solvent system. A total of 45.6 mg of plantamajoside and 293.8 mg of acteoside were purified from 1341 mg of the n-butanol extract of P. asiatica, with a purity of 〉93.3% as determined by HPLC. The HPCPC fractions were analyzed by HPLC-DAD and the structures were identified by their retention time, UV, electrospray ionization multi stage tandem mass spectrometry（ESI-MSn） in the negative ion mode, and confirmed by NMR experiments. The characteristic fragment ions of ESI-MS of the two PhGs isolated from Plantago asiatica were discussed, which are specific and useful for the identification of the structures of PhGs.
基金supported financially by the grants from the Chinese Academy of Sciences(KSCX2-EW-Q-10 and KSCX1-YW-R-24)the NSFC(No.20802082 and 30830115)+1 种基金the Major State Basic Research Development Program of China(No.2009CB522303 and 2009CB940900)the project of recruited top talent of sciences and technology of Yunnan Province(2006PY01-47).
文摘Four new indole alkaloids,plasiaticines A-D(1-4),together with two known ones,were isolated from the seeds of Plantago asiatica.The structures of the new compounds were elucidated on the basis of comprehensive analysis of spectroscopic data.All compounds were tested for their cytotoxic activity,and all compounds except 4 were tested for their acetylcholinesterase(AChE)inhibitory activities.
基金the support of the National Natural Science Foundation of China(No.8187296 Authors 9)the Science and Youth Innovation Promotion Association of the CAS(Grant No.2019227)。
文摘Objective:This study aimed to elucidate the active components and mechanisms of Plantago asiatica L.(PAL)in the treatment of gout.Materials and Methods:Based on its polarity,PAL was partitioned into three parts,P1,P2,and P3,through gradient elution using AB-8 macroporous resin columns.The primary constituents of these fractions include iridoids,phenylethanoid glucosides,and flavonoids.Subsequently,a comprehensive in vitro and in vivo screening and evaluation model was developed considering the fundamental pathological processes of gout,including the inhibition of uric acid(UA)production,augmentation of UA excretion,and anti-inflammatory effects.This model was used to analyze the anti-gout activity of P1,P2,P3,and the overall extracts derived from PAL.Results:All the PAL parts exhibited specific therapeutic properties as part of gout therapy.Specifically,the P2 part demonstrated the ability to diminish interleukin-1 beta levels,lower UA concentrations,suppress joint inflammation in rats,and restore abnormal parameters associated with gout,such as creatinine and urea nitrogen.These findings highlight the significant effect of P2 on gout treatment.Conclusions:Following a thorough assessment of the outcomes derived from diverse anti-gout experiments,P2 exhibited superior anti-gout properties compared to the remaining parts.P2 demonstrated a restorative effect on the aberrant parameters associated with gout,thereby comprehensively elucidating the therapeutic mechanism of P2 against gout.This study provides substantial evidence and serves as a reference for future investigations regarding the material foundation of PAL in gout treatment.
文摘Box dimension and information dimension based on individual and leaf number were applied to analyze the distribution patterns of Plantato asiatica and P. viriginica in suburbs of Jinhua. The results showed that both the box dimension and the information dimention of P. virginica were obviously higher than those of P. asiatica in the high human-distributed environment. P. asiatica was mostly distributed in the environment with compacted soil. The information dimension on the basis of leaf probability in the boxes showed that the populations of P. virginica and P. asiatica in low density could exploit environmental resources effectively.
基金Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (JWSL-2009-1590)Key Laboratory of Natural Resources of Changbai Mountain & Functional Molecules (Yanbian University),Ministry of Education,China (CSSHZ-2009-05)
文摘Objective To identify the active compounds for protein tyrosine phosphatase 1B(PTP1B) from the seeds of Plantago asiatica.Methods Bioassay-guided fractionation resulted in the isolation of iridoid glucosides(1-5) with PTP1B inhibitory activity.Results Five compounds were identified as desacetylhookerioside(1),melittoside(2),geniposidic acid(3),10-O-acetyl-geniposidic acid(4),and alpinoside(5).Conclusion Isolated compounds 35 inhibit PTP1B with IC50 values ranged from(16.3 ± 1.1) to(19.8 ± 1.2) μmol/L.
