摘要
运用 18个有效的 10mer 寡核苷酸引物对浙江产车前 (Plantagoasiatica)的 8个种群基因组DNA进行了PCR扩增 ,共检测到 186个位点 ,其中多态位点 12 5个 ,占 6 8.70 % .应用Jaccard公式计算了 8个种群在 12 5个位点上的相似性 .以此为基础 ,应用主坐标排序 ,作出了 8个种群遗传分化的三维排序图 .结果表明 ,8个种群的遗传分化与地理位置、海拔高度有联系 .
Eight populations of Plantago asiatica collected from Zhejiang were analyzed using random amplified polymorphic DNA (RAPD). Eighteen of the 60 random primers were selected.186 bands were amplified by the 18 primers ,and 68.70% of them were polymorphic. Jaccard coefficient was applied to compare the genetic of these eight populations based on the RAPD data. Three-dimensional ordination plot of the eight populations based on the Jaccard coefficient was constructed by using principal axes analysis (PAA). The results showed that the genetic differentiation of eight populations could be explained to a certain extent by their geographical location and altitude .
出处
《应用生态学报》
CAS
CSCD
2002年第10期1283-1286,共4页
Chinese Journal of Applied Ecology
基金
国家教育部高等学校骨干教师资助计划项目 (No .182 1) .
