One new Iriterpene saponin was isolated from Panaxjaponicus C. A. Meyer var major （Burk.） C. Y. Wu et K. M. Feng, and established as oleanolic acid 3-O-[β-D-glucopyranosyl-（1 →2）-β-D-glucuronopyranosyl-6＇-O-n-...One new Iriterpene saponin was isolated from Panaxjaponicus C. A. Meyer var major （Burk.） C. Y. Wu et K. M. Feng, and established as oleanolic acid 3-O-[β-D-glucopyranosyl-（1 →2）-β-D-glucuronopyranosyl-6＇-O-n-butyl ester] which showed mod- erate antitumor activities against the A2780 cells and OVCAR-3 cells. Its structure was established by means of spectral data, particularly NMR, including HSQC and HMBC techniques.展开更多
Panax japonicus and its approximation varieties,such as Rhizoma Panacis Majoris and Panax japonicus C. A. Mey. var.major （Burk.） C.Y. Wu et K.M. Feng belong to Panax,which are less commonly used traditional Chinese ...Panax japonicus and its approximation varieties,such as Rhizoma Panacis Majoris and Panax japonicus C. A. Mey. var.major （Burk.） C.Y. Wu et K.M. Feng belong to Panax,which are less commonly used traditional Chinese medicine. Because of similar traits and effectiveness,they were always used as one type of medicine for a long time. Aiming at this phenomenon,the chemical composition and contents of P. japonicus and its approximation varieties from different area were compared in order to provide a chemical basis for clarifying the classification of the genus.展开更多
Water-soluble polysaccharides were prepared from Panax japonicus by hot water extraction and ethanol precipitation.The polysaccharides were further purified by ion exchange chromatography to obtain neutral and acidic ...Water-soluble polysaccharides were prepared from Panax japonicus by hot water extraction and ethanol precipitation.The polysaccharides were further purified by ion exchange chromatography to obtain neutral and acidic polysaccharides.The neutral polysaccharide fraction mainly contained Glc(90.2%),which was a glucan fraction.The acidic polysaccharide fraction mainly contained GalA(43.6%),Gal(21.7%),and Ara(15.4%),with a degree of methyl-esterification of 20.3%,which was a pectic polysaccharide.The acidic polysaccharide of Panax japonicus could effectively inhibit the replication of human seasonal influenza virus H1N1 and canine influenza virus H3N2 in MDCK cells and A549 cells and significantly reduce the virus titer in infected cells.It also effectively inhibited the number of infected cells of the SARS-CoV-2 South Africa strain and the Omicron strain.The acid polysaccharide of Panax japonicus showed good efficacy against influenza virus and COVID-19 infection,which could be used as a potential antiviral candidate drug molecule in the future.展开更多
[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogo...[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, and Allium macrostemon. [Method] Herbal active ingredients were extracted by water boiling method and a bacteriostatic experiment was per- formed by oxford cup diffusion method, to select Chinese herbs with antibacterial effects. MIC values of seven kinds of Chinese herbal medicine were determined by in vitro half-fold dilution method. [Results] Root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, Allium macrostemon, Juncus effusus and Xanthi- um sibiricum had bacteriostatic effects on Escherichia coil and Staphylococcus au- reus in varying degrees. Xanthium and leaf of Ophiopogon japonicus performed bet- ter on Escherichia coil, with MIC values at 7.81 and 15.63 mg/ml, respectively. Panax pseudoginseng and Xanthium performed better on Staphylococcus aureus with MIC values at 15.63 and 31.25 mg/ml. And the drug＇s effect was positively correlated with the concentration. [Conclusion] Xanthium sibiricum is the best bacte- riostatic effect, followed by leaf of Ophiopogon japonicus, and Juncus effusus per- forms the poorest. Panax pseudoginseng showed the best bacteriostatic effect, fol- lowed by Xanthium sibiricum and Juncus effusus performs the poorest. These pro- vide references for clinic safety.展开更多
Three-year-old Panax japonicus was exposed to elevated CO_(2) concentrations using open-top chambers:ambient CO_(2)(aCO_(2)),moderately elevated(e1CO_(2),550μmol/mol),and highly elevated(e2CO_(2),750μmol/mol).Gas ex...Three-year-old Panax japonicus was exposed to elevated CO_(2) concentrations using open-top chambers:ambient CO_(2)(aCO_(2)),moderately elevated(e1CO_(2),550μmol/mol),and highly elevated(e2CO_(2),750μmol/mol).Gas exchange parameters,photosynthetic pigments,sugar accumulation,and total saponin content were measured to assess the effects of CO_(2) enrichment on photosynthesis,sugar metabolism,and saponin biosynthesis.The e1CO_(2) treatment significantly increased net photosynthetic rate(by 17.22%at 36 days and 69.62%at 92 days),chlorophyll a content,and soluble sugar,sucrose,and starch accumulation.Key sugar metabolism enzymes,including sucrose phosphate synthase(SPS),also showed enhanced activity.Consequently,underground rhizome total saponins rose significantly by 15.16%.In contrast,e2CO_(2) initially(36 days)stimulated photosynthesis but lost this effect over prolonged exposure(92 days),with no significant impact on photosynthetic parameters,pigments,or sugar levels.Correlation analysis indicated that rhizome saponin content was positively associated with leaf sucrose levels and sucrose synthase(synthetic direction)activity.These findings suggest that moderate CO_(2) elevation(e1CO_(2))enhances P.japonicus photosynthesis and sugar metabolism,driving greater saponin accumulation.However,high CO_(2)(e2CO_(2))has transient benefits,with stimulatory effects diminishing over time.展开更多
焦虑症是高发的心理疾病,研究表明肥胖是导致焦虑症的重要危险因素。该研究探讨竹节参总皂苷(saponins from Panax japonicus,SPJ)对高脂饮食小鼠焦虑症的改善作用及机制。将50只C57BL/6J小鼠随机分为正常组、高脂饮食组、SPJ低剂量组、...焦虑症是高发的心理疾病,研究表明肥胖是导致焦虑症的重要危险因素。该研究探讨竹节参总皂苷(saponins from Panax japonicus,SPJ)对高脂饮食小鼠焦虑症的改善作用及机制。将50只C57BL/6J小鼠随机分为正常组、高脂饮食组、SPJ低剂量组、SPJ高剂量组。第12周从高脂饮食组取6只小鼠,分为对照组、外源性给予成纤维细胞生长因子21(FGF21)组。旷场和高架十字迷宫实验评价小鼠焦虑样行为;苏木素-伊红(HE)染色和油红O染色观察肝脏、脂肪的病理变化;葡萄糖耐量实验和胰岛素耐量实验评估小鼠糖代谢水平;蛋白免疫印迹法检测肝脏和皮层中FGF21及下游相关蛋白和大脑皮层中脑源性神经营养因子(BDNF)、突触后膜蛋白4(DLG4)和突触素(SYP)表达;实时荧光定量PCR(qPCR)检测肝脏和皮层中FGF21及相关受体基因表达;免疫荧光检测大脑皮层神经元激活物(c-Fos)、FGF21、FGF21辅助受体β-klotho的表达。结果显示,SPJ可显著改善高脂饮食小鼠在高架十字迷宫的开臂区及旷场中心区的活动频率,并上调BDNF、DLG4和SYP表达,有效改善高脂饮食小鼠的焦虑样行为。与正常组相比,高脂饮食组小鼠的肝脏及大脑皮层中FGF21的表达上调,成纤维细胞生长因子受体1(FGFR1)与β-klotho的表达显著下降,提示高脂饮食小鼠呈FGF21抵抗状态;SPJ可显著上调高脂饮食小鼠β-klotho水平,逆转FGF21抵抗。进一步与外源性给予FGF21进行对比,显示SPJ激活大脑皮层区域与其一致,同时SPJ能促进大脑皮层中c-Fos与β-klotho阳性细胞数量及共定位。综上所述,SPJ可有效改善高脂饮食小鼠的焦虑样行为,其机制与上调大脑β-klotho表达,逆转FGF21抵抗,进而激活大脑皮层、杏仁核神经元有关。展开更多
目的研究五加科人参属植物珠子参(Panax japonicus C.A.Mey.var.major(Burk.)C.Y.Wu et K.M.Feng.)叶的化学成分。方法采用大孔树脂、MCI、硅胶柱色谱、半制备液相等手段对珠子参叶70%乙醇部位进行了精细的分离与纯化。然后,利用1D/2D-...目的研究五加科人参属植物珠子参(Panax japonicus C.A.Mey.var.major(Burk.)C.Y.Wu et K.M.Feng.)叶的化学成分。方法采用大孔树脂、MCI、硅胶柱色谱、半制备液相等手段对珠子参叶70%乙醇部位进行了精细的分离与纯化。然后,利用1D/2D-NMR、高分辨质谱等多种波谱技术,依据其理化特性,来确认该化合物的结构。结果分离鉴定得到10个化合物,分别为20(R)-原人参三醇(1)、20(R)-人参三醇(2)、20(R)-达玛烷-3β,6α,12β,20,25-戊醇(3)、达玛-20(22)E,24-二烯-3β,6α,12β-三醇(4)、达玛-20(22)Z,24-二烯-3β,6α,12β-三醇(5)、27-demethyl-(E,E)-20(22),23-dien-3β,6α,12β-trihydroxydammar-25-one(6)、3β,6α,12β-triol-22,23,24,25,26,27-hexanordammaran-20-one(7)、山柰酚(8)、5-羟甲基-2-呋喃甲醛(9)、4-甲氧基苯酚(10)。结论化合物2、3、6~10为首次从珠子参中分离鉴定。展开更多
竹节参作为国家重点二级保护野生植物,兼具北药人参和南药三七的特点;珠子参作为秦巴特色中草药资源“太白七药”之一,是药效好、价格高的药食两用野生名贵药材。但是目前对竹节参、珠子参的研究主要集中在药用部位,从而忽略地上部位的...竹节参作为国家重点二级保护野生植物,兼具北药人参和南药三七的特点;珠子参作为秦巴特色中草药资源“太白七药”之一,是药效好、价格高的药食两用野生名贵药材。但是目前对竹节参、珠子参的研究主要集中在药用部位,从而忽略地上部位的药用价值,导致资源浪费。竹节参、珠子参地上部位含有皂苷类、黄酮类、挥发油等化学成分,具有抗血栓、抗癌、护肝、抗炎镇痛、提高免疫力等药理活性,开发前景广阔。通过查询中草药书籍及中国知网、Web of Science、Pub Med、中国中医药数据库、中药材植物网等科学数据库,对竹节参、珠子参非药用部位的化学成分、药理作用及资源利用方面进行梳理与归纳,旨在为竹节参和珠子参非药用部位的化学研究、资源价值发现、产品开发利用等方面提供参考和依据。展开更多
基金financially supported by Chinese Academy of Nutritional Sciences,Shanghai Institutes for Biological Sciences
文摘One new Iriterpene saponin was isolated from Panaxjaponicus C. A. Meyer var major （Burk.） C. Y. Wu et K. M. Feng, and established as oleanolic acid 3-O-[β-D-glucopyranosyl-（1 →2）-β-D-glucuronopyranosyl-6＇-O-n-butyl ester] which showed mod- erate antitumor activities against the A2780 cells and OVCAR-3 cells. Its structure was established by means of spectral data, particularly NMR, including HSQC and HMBC techniques.
基金Supported by the National Natural Foundation of China(30873383)~~
文摘Panax japonicus and its approximation varieties,such as Rhizoma Panacis Majoris and Panax japonicus C. A. Mey. var.major （Burk.） C.Y. Wu et K.M. Feng belong to Panax,which are less commonly used traditional Chinese medicine. Because of similar traits and effectiveness,they were always used as one type of medicine for a long time. Aiming at this phenomenon,the chemical composition and contents of P. japonicus and its approximation varieties from different area were compared in order to provide a chemical basis for clarifying the classification of the genus.
基金supported by National Key Research and Development Program of China(2023YFC0871100)
文摘Water-soluble polysaccharides were prepared from Panax japonicus by hot water extraction and ethanol precipitation.The polysaccharides were further purified by ion exchange chromatography to obtain neutral and acidic polysaccharides.The neutral polysaccharide fraction mainly contained Glc(90.2%),which was a glucan fraction.The acidic polysaccharide fraction mainly contained GalA(43.6%),Gal(21.7%),and Ara(15.4%),with a degree of methyl-esterification of 20.3%,which was a pectic polysaccharide.The acidic polysaccharide of Panax japonicus could effectively inhibit the replication of human seasonal influenza virus H1N1 and canine influenza virus H3N2 in MDCK cells and A549 cells and significantly reduce the virus titer in infected cells.It also effectively inhibited the number of infected cells of the SARS-CoV-2 South Africa strain and the Omicron strain.The acid polysaccharide of Panax japonicus showed good efficacy against influenza virus and COVID-19 infection,which could be used as a potential antiviral candidate drug molecule in the future.
文摘[Objective] The aim was to explore the effects of seven kinds of Chinese herbal medicine on Escherichia coil and Staphylococcus aureus in terms of unilater- al bacteriostatic effect, such as root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, and Allium macrostemon. [Method] Herbal active ingredients were extracted by water boiling method and a bacteriostatic experiment was per- formed by oxford cup diffusion method, to select Chinese herbs with antibacterial effects. MIC values of seven kinds of Chinese herbal medicine were determined by in vitro half-fold dilution method. [Results] Root and leaf of Ophiopogon japonicus, honeycomb, Panax pseudoginseng, Allium macrostemon, Juncus effusus and Xanthi- um sibiricum had bacteriostatic effects on Escherichia coil and Staphylococcus au- reus in varying degrees. Xanthium and leaf of Ophiopogon japonicus performed bet- ter on Escherichia coil, with MIC values at 7.81 and 15.63 mg/ml, respectively. Panax pseudoginseng and Xanthium performed better on Staphylococcus aureus with MIC values at 15.63 and 31.25 mg/ml. And the drug＇s effect was positively correlated with the concentration. [Conclusion] Xanthium sibiricum is the best bacte- riostatic effect, followed by leaf of Ophiopogon japonicus, and Juncus effusus per- forms the poorest. Panax pseudoginseng showed the best bacteriostatic effect, fol- lowed by Xanthium sibiricum and Juncus effusus performs the poorest. These pro- vide references for clinic safety.
基金funded by the Guizhou Province Technology Plan Project,Qiankehe Basic-ZK[2023]General 023,Key 002 and Guizhou Province Project for the Growth of Young Scientific and Technological Talents in General Higher Education Institutions,QianJiao He Foundation,Grant No.(2022)029.
文摘Three-year-old Panax japonicus was exposed to elevated CO_(2) concentrations using open-top chambers:ambient CO_(2)(aCO_(2)),moderately elevated(e1CO_(2),550μmol/mol),and highly elevated(e2CO_(2),750μmol/mol).Gas exchange parameters,photosynthetic pigments,sugar accumulation,and total saponin content were measured to assess the effects of CO_(2) enrichment on photosynthesis,sugar metabolism,and saponin biosynthesis.The e1CO_(2) treatment significantly increased net photosynthetic rate(by 17.22%at 36 days and 69.62%at 92 days),chlorophyll a content,and soluble sugar,sucrose,and starch accumulation.Key sugar metabolism enzymes,including sucrose phosphate synthase(SPS),also showed enhanced activity.Consequently,underground rhizome total saponins rose significantly by 15.16%.In contrast,e2CO_(2) initially(36 days)stimulated photosynthesis but lost this effect over prolonged exposure(92 days),with no significant impact on photosynthetic parameters,pigments,or sugar levels.Correlation analysis indicated that rhizome saponin content was positively associated with leaf sucrose levels and sucrose synthase(synthetic direction)activity.These findings suggest that moderate CO_(2) elevation(e1CO_(2))enhances P.japonicus photosynthesis and sugar metabolism,driving greater saponin accumulation.However,high CO_(2)(e2CO_(2))has transient benefits,with stimulatory effects diminishing over time.
文摘焦虑症是高发的心理疾病,研究表明肥胖是导致焦虑症的重要危险因素。该研究探讨竹节参总皂苷(saponins from Panax japonicus,SPJ)对高脂饮食小鼠焦虑症的改善作用及机制。将50只C57BL/6J小鼠随机分为正常组、高脂饮食组、SPJ低剂量组、SPJ高剂量组。第12周从高脂饮食组取6只小鼠,分为对照组、外源性给予成纤维细胞生长因子21(FGF21)组。旷场和高架十字迷宫实验评价小鼠焦虑样行为;苏木素-伊红(HE)染色和油红O染色观察肝脏、脂肪的病理变化;葡萄糖耐量实验和胰岛素耐量实验评估小鼠糖代谢水平;蛋白免疫印迹法检测肝脏和皮层中FGF21及下游相关蛋白和大脑皮层中脑源性神经营养因子(BDNF)、突触后膜蛋白4(DLG4)和突触素(SYP)表达;实时荧光定量PCR(qPCR)检测肝脏和皮层中FGF21及相关受体基因表达;免疫荧光检测大脑皮层神经元激活物(c-Fos)、FGF21、FGF21辅助受体β-klotho的表达。结果显示,SPJ可显著改善高脂饮食小鼠在高架十字迷宫的开臂区及旷场中心区的活动频率,并上调BDNF、DLG4和SYP表达,有效改善高脂饮食小鼠的焦虑样行为。与正常组相比,高脂饮食组小鼠的肝脏及大脑皮层中FGF21的表达上调,成纤维细胞生长因子受体1(FGFR1)与β-klotho的表达显著下降,提示高脂饮食小鼠呈FGF21抵抗状态;SPJ可显著上调高脂饮食小鼠β-klotho水平,逆转FGF21抵抗。进一步与外源性给予FGF21进行对比,显示SPJ激活大脑皮层区域与其一致,同时SPJ能促进大脑皮层中c-Fos与β-klotho阳性细胞数量及共定位。综上所述,SPJ可有效改善高脂饮食小鼠的焦虑样行为,其机制与上调大脑β-klotho表达,逆转FGF21抵抗,进而激活大脑皮层、杏仁核神经元有关。
文摘目的研究五加科人参属植物珠子参(Panax japonicus C.A.Mey.var.major(Burk.)C.Y.Wu et K.M.Feng.)叶的化学成分。方法采用大孔树脂、MCI、硅胶柱色谱、半制备液相等手段对珠子参叶70%乙醇部位进行了精细的分离与纯化。然后,利用1D/2D-NMR、高分辨质谱等多种波谱技术,依据其理化特性,来确认该化合物的结构。结果分离鉴定得到10个化合物,分别为20(R)-原人参三醇(1)、20(R)-人参三醇(2)、20(R)-达玛烷-3β,6α,12β,20,25-戊醇(3)、达玛-20(22)E,24-二烯-3β,6α,12β-三醇(4)、达玛-20(22)Z,24-二烯-3β,6α,12β-三醇(5)、27-demethyl-(E,E)-20(22),23-dien-3β,6α,12β-trihydroxydammar-25-one(6)、3β,6α,12β-triol-22,23,24,25,26,27-hexanordammaran-20-one(7)、山柰酚(8)、5-羟甲基-2-呋喃甲醛(9)、4-甲氧基苯酚(10)。结论化合物2、3、6~10为首次从珠子参中分离鉴定。
文摘竹节参作为国家重点二级保护野生植物,兼具北药人参和南药三七的特点;珠子参作为秦巴特色中草药资源“太白七药”之一,是药效好、价格高的药食两用野生名贵药材。但是目前对竹节参、珠子参的研究主要集中在药用部位,从而忽略地上部位的药用价值,导致资源浪费。竹节参、珠子参地上部位含有皂苷类、黄酮类、挥发油等化学成分,具有抗血栓、抗癌、护肝、抗炎镇痛、提高免疫力等药理活性,开发前景广阔。通过查询中草药书籍及中国知网、Web of Science、Pub Med、中国中医药数据库、中药材植物网等科学数据库,对竹节参、珠子参非药用部位的化学成分、药理作用及资源利用方面进行梳理与归纳,旨在为竹节参和珠子参非药用部位的化学研究、资源价值发现、产品开发利用等方面提供参考和依据。
