For efficient mucosal vaccine delivery, nanoparticulate antigens are better taken by microfold cells in the nasal associated lymphoid tissue and also dendritic cells. Nanoparticles based on polymers such as chitosan(C...For efficient mucosal vaccine delivery, nanoparticulate antigens are better taken by microfold cells in the nasal associated lymphoid tissue and also dendritic cells. Nanoparticles based on polymers such as chitosan(CHT) and its water soluble derivative, trimethylchitosan(TMC), could be successfully used as carrier/adjuvant for this purpose. Sodium alginate, a negatively charged biopolymer, could modify the immunostimulatory properties of CHT and TMC NPs and increase their stability. Sodium alginate(ALG)-coated chitosan(CHT)and trimethylchitosan(TMC) nanoparticles(NPs) loaded with inactivated PR8 influenza virus were successfully prepared by direct coating of the virus with CHT or TMC polymers to evaluate their immunoadjuvant potential after nasal immunization. After nasal immunizations in BALB/c mice, PR8-CHT formulation elicited higher IgG2 a and Ig G1 antibody titers compared with PR8-TMC. ALG coating of this formulation(PR8-CHT-ALG) significantly decreased the antibody titers and a less immune response was induced than PR8-TMC-ALG formulation. PR8-TMC-ALG formulation showed significantly higher Ig G2 a/Ig G1 ratio, as criteria for Th1-type immune response, compared with PR8-CHT-ALG and PR8 virus alone. Altogether, the PR8-TMC-ALG formulation could be considered as an efficient intranasal antigen delivery system for nasal vaccines.展开更多
[目的/意义]基于论文被引频次8个区段百分位数排序(percentile rank 8,PR8)赋分,尝试构建新的跨学科期刊评价指标:期刊PR8指数(journal index for PR8,JIPR8),并检验JIPR8的跨学科期刊评价效果。[方法/过程]选择JCR中8个学科301种期刊...[目的/意义]基于论文被引频次8个区段百分位数排序(percentile rank 8,PR8)赋分,尝试构建新的跨学科期刊评价指标:期刊PR8指数(journal index for PR8,JIPR8),并检验JIPR8的跨学科期刊评价效果。[方法/过程]选择JCR中8个学科301种期刊作为研究对象,分别计算每种期刊的JIPR8,并与其他几个跨学科期刊评价指标进行比较,检验JIPR8跨学科期刊评价的敏感度和稳定性,以及与其他跨学科期刊评价指标的相关性。[结果/结论]在选择的所有指标中,8个学科301种期刊JIPR8的变异程度最低,说明其用于跨学科期刊评价的稳定性最好;不同分区期刊(Q1、Q2、Q3和Q4)JIPR8的组间差异性较为明显,仅次于期刊影响因子百分位(journal impact factor pencentile,JIFP),表明其对优秀和一般期刊的区分度较好。认为JIPR8是一个非常理想的跨学科期刊评价指标。展开更多
基金part of Amir-Hossein Sabbaghi Pharm.D.thesis(Grant number:911042)supported by Vice Chancellor for Research,Mashhad University of Medical Sciences
文摘For efficient mucosal vaccine delivery, nanoparticulate antigens are better taken by microfold cells in the nasal associated lymphoid tissue and also dendritic cells. Nanoparticles based on polymers such as chitosan(CHT) and its water soluble derivative, trimethylchitosan(TMC), could be successfully used as carrier/adjuvant for this purpose. Sodium alginate, a negatively charged biopolymer, could modify the immunostimulatory properties of CHT and TMC NPs and increase their stability. Sodium alginate(ALG)-coated chitosan(CHT)and trimethylchitosan(TMC) nanoparticles(NPs) loaded with inactivated PR8 influenza virus were successfully prepared by direct coating of the virus with CHT or TMC polymers to evaluate their immunoadjuvant potential after nasal immunization. After nasal immunizations in BALB/c mice, PR8-CHT formulation elicited higher IgG2 a and Ig G1 antibody titers compared with PR8-TMC. ALG coating of this formulation(PR8-CHT-ALG) significantly decreased the antibody titers and a less immune response was induced than PR8-TMC-ALG formulation. PR8-TMC-ALG formulation showed significantly higher Ig G2 a/Ig G1 ratio, as criteria for Th1-type immune response, compared with PR8-CHT-ALG and PR8 virus alone. Altogether, the PR8-TMC-ALG formulation could be considered as an efficient intranasal antigen delivery system for nasal vaccines.
文摘[目的/意义]基于论文被引频次8个区段百分位数排序(percentile rank 8,PR8)赋分,尝试构建新的跨学科期刊评价指标:期刊PR8指数(journal index for PR8,JIPR8),并检验JIPR8的跨学科期刊评价效果。[方法/过程]选择JCR中8个学科301种期刊作为研究对象,分别计算每种期刊的JIPR8,并与其他几个跨学科期刊评价指标进行比较,检验JIPR8跨学科期刊评价的敏感度和稳定性,以及与其他跨学科期刊评价指标的相关性。[结果/结论]在选择的所有指标中,8个学科301种期刊JIPR8的变异程度最低,说明其用于跨学科期刊评价的稳定性最好;不同分区期刊(Q1、Q2、Q3和Q4)JIPR8的组间差异性较为明显,仅次于期刊影响因子百分位(journal impact factor pencentile,JIFP),表明其对优秀和一般期刊的区分度较好。认为JIPR8是一个非常理想的跨学科期刊评价指标。
