摘要
目的评价疏风解毒胶囊在体内对H1N1流感病毒感染的防治作用。方法研究采用甲型H1N1流感病毒FM1株和PR8株滴鼻感染免疫低下小鼠造成肺炎模型,分别进行治疗性和预防性给药,观察小鼠肺指数、病死率,并进一步进行分子机理研究。结果①致免疫低下小鼠肺炎模型实验中,治疗性给予疏风解毒胶囊4d后,三个剂量组肺指数、肺组织中的病毒载量均明显降低;FM1株三个剂量组动物的死亡数均显著降低,PR8株中剂量组动物的存活天数显著增加;②致正常小鼠肺炎模型实验中,治疗性给药可明显降低肺组织中的病毒载量;提高小鼠肺组织IFN-γ含量,增加血清中SOD活力;并降低血清中TNF-α的含量。结论疏风解毒胶囊可通过影响小鼠免疫功能,改善流感病毒引起的小鼠肺炎症状;降低H1N1感染小鼠的肺指数,对病毒性感冒有较好的治疗;并可明显降低感染动物的死亡率,延长存活天数。
Objective To evaluate the efficacy of in vivo treatment and protection of shufengjiedu capsule on H1N1 infection. Methods The influenza viruses FM1 and PR8 of H1N1 were adopted to establish pneumonia models in immunocompromised mice by nasal dropping method. The medications for treatment and prevention were administered separately. The lung index and death rate were observed. Additionally, the molecular mechanism was further analyzed. Results 1. In the experimental research in pneumonia models of immunocompromised mice,after 4 days of treatment with shufengjiedu capsule, the lung index and viral load in lung tissue of three dose groups decreased apparently. The death numbers of animal in FM1 three dose groups reduced significantly. The survival days of animal in middle-dose group of PR8 increased significant ly. 2. In the experimental research in pneumonia models of immunocompromised mice, the medication for treatment reduced apparently viral load in lung tissue, improved IFN -γ level in lung tissue and SOD activity in serum,and decreased TNF-α level. Conclusion By impacting immune function of mice,shufengjiedu capsule improves in pneumonia symptoms induced by influenza virus in mice, decreases lung index in mice with H1N1 infection and is markedly effective on influenza. Additionally, this drug decreases apparently the death rate of infected animals and prolongs survival days in evaluation.
出处
《世界中西医结合杂志》
2010年第2期107-110,共4页
World Journal of Integrated Traditional and Western Medicine
基金
北京市科技计划
科技部科技计划--防治甲型H1N1流感专项
