PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress.However,there has been very little research on the function of this...PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress.However,there has been very little research on the function of this family gene in tomatoes,which limits its application in germplasm resource improvement.Therefore,the PLATZ gene family was identified and analyzed in tomato,and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene function.In this study,the PLATZ family members of tomato were identified in the whole genome,and 19 SlPLATZ genes were obtained.Functional prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern analysis.SlPLATZ genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene resources.SlPLATZ17 was selected for functional verification by experiment-based analysis.The results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato plants.Tomato plants overexpressing SlPLATZ17 had larger flower sizes and long,thin petals,adjacent petals were not connected at the base,and the stamen circumference was smaller.This study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening.展开更多
Plant AT-rich sequence and zinc binding(PLATZ)transcription factors are a class of plant specific zincdependent DNA-binding proteins that function in abiotic stress response and plant development.In this study,31 GmPL...Plant AT-rich sequence and zinc binding(PLATZ)transcription factors are a class of plant specific zincdependent DNA-binding proteins that function in abiotic stress response and plant development.In this study,31 GmPLATZ genes were identified in soybean.GmPLATZ17 was down-regulated by drought and exogenous abscisic acid.Transgenic Arabidopsis and soybean hairy roots overexpressing GmPLATZ17 showed drought sensitivity and inhibition of stress-associated gene transcription.In contrast,suppressed expression of GmPLATZ17 led to increased drought tolerance in transgenic soybean hairy roots.The GmPLATZ17 protein was verified to interact physically with the GmDREB5 transcription factor,and overexpression of GmDREB5 increased drought tolerance in soybean hairy roots.Interaction of GmPLATZ17 with GmDREB5 was shown to interfere with the DRE-binding activity of GmDREB5,suppressing downstream stress-associated gene expression.These results show that GmPLATZ17 inhibits drought tolerance by interacting with GmDREB5.This study sheds light on PLATZ transcription factors and the function of GmPLATZ17 in regulating drought sensitivity.展开更多
Plant AT-rich sequence and zinc-binding protein(PLATZ)is a plant transcription factor that has been studied in corn.PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression.I...Plant AT-rich sequence and zinc-binding protein(PLATZ)is a plant transcription factor that has been studied in corn.PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression.It is involved in the regulation of dehydration tolerance in seeds.In this study,we performed bioinformatics analysis to identify and characterize wheat PLATZ(TaPLATZ)genes.We identified 49 wheat PLATZ genes by searching the wheat genome by using known PLATZ gene sequences from rice,Arabidopsis,and maize.Phylogenetic analysis on PLATZ gene sequences from different species was performed.We found that PLATZs could be divided into three groups.The chromosome(chr)distribution analysis revealed that the 49 identified wheat PLATZ genes are distributed in 15 chrs.Gene structure and motif analyses indicated that most PLATZ genes possess conserved exon/intron arrangements and motif compositions.Our analysis of transcriptional data indicated that several wheat PLATZ genes may play an important role in abiotic stress resistance given that they are expressed under salt stress.The results of qRT-PCR further confirmed that TaPLATZ is involved in plant abiotic stress and is also related to the cell differentiation of plant tissues.Our results lay the foundation for further studies on the function of the wheat PLATZ gene family.展开更多
Grain size is an important agronomic trait that largely determines grain yield in rice(Oryza sativa L.).The genes encoding the Growth Regulating Factors(GRFs)and G-proteins are major regulators for grain length regula...Grain size is an important agronomic trait that largely determines grain yield in rice(Oryza sativa L.).The genes encoding the Growth Regulating Factors(GRFs)and G-proteins are major regulators for grain length regulation,but how these pathways are coordinated in plants remains elusive.Here,we described OsPLATZ1 as a transcriptional activator,a member of the Plant AT-rich sequence-and Zinc-binding family proteins in rice that positively regulates grain length.OsPLATZ1 interacted with multiple GRFs,and the OsPLATZ1-Os GRF4 complex bound to regulatory regions in the promoter of the G-protein gene DENSE AND ERECT PANICLE1(DEP1)to enhance its expression,thereby regulating grain length.We used gene editing to modify the OsPLATZ1 promoter regulatory region and obtained mutant lines with downregulated or upregulated OsPLATZ1 expression depending on the type of editing event.One of these mutant lines had changes in multiple agronomic traits and improved grain yield and grain appearance quality.Our findings reveal a new regulatory module in which OsPLATZ1 connects the GRFs and G-protein signaling pathways to regulate grain length and suggest that finely modulating OsPLATZ1activity might be a promising molecular breeding approach.展开更多
Saline-alkaline soils are a major environmental problem that limit plant growth and crop productivity.Plasma membrane H^(+)-ATPases and the salt overly sensitive(SOS)signaling pathway play important roles in plant res...Saline-alkaline soils are a major environmental problem that limit plant growth and crop productivity.Plasma membrane H^(+)-ATPases and the salt overly sensitive(SOS)signaling pathway play important roles in plant responses to saline-alkali stress.However,little is known about the functional genes and mechanisms regulating the transcription of H^(+)-ATPases and SOS pathway genes under saline–alkali stress.In the present study,we identified that the plant AT-rich sequence and zinc-binding(TaPLATZ2)transcription factor are involved in wheat response to saline-alkali stress by directly suppressing the expression of TaHA2/TaSOS3.The knockdown of TaPLATZ2 enhances salt and alkali stress tolerance,while overexpression of TaPLATZ2 leads to salt and alkali stress sensitivity in wheat.In addition,TaWRKY55 directly upregulated the expression of TaPLATZ2 during saline-alkali stress.Through knockdown and overexpression of Ta WRKY55 in wheat,Ta WRKY55 was shown to negatively modulate salt and alkali stress tolerance.Genetic analyses confirmed that Ta PLATZ2 functions downstream of Ta WRKY55 in response to salt and alkaline stresses.These findings provide a TaWRKY55–TaPLATZ2–TaHA2/TaSOS3 regulatory module that regulates wheat responses to saline-alkali stress.展开更多
基金support from the National Natural Science Foundation of China(32102390 and 32072589)the China Agriculture Research System(CARS-23-A11)+1 种基金the Heilongjiang Provincial Natural Science Foundation of China(YQ2021C013)the Northeast Agricultural University Scholars Program(20XG28),China。
文摘PLATZ is a novel zinc finger DNA-binding protein that plays an important role in regulating plant growth and development and resisting abiotic stress.However,there has been very little research on the function of this family gene in tomatoes,which limits its application in germplasm resource improvement.Therefore,the PLATZ gene family was identified and analyzed in tomato,and its roles were predicted and verified to provide a basis for in-depth research on SlPLATZ gene function.In this study,the PLATZ family members of tomato were identified in the whole genome,and 19 SlPLATZ genes were obtained.Functional prediction was conducted based on gene and promoter structure analysis and RNA-seq-based expression pattern analysis.SlPLATZ genes that responded significantly under different abiotic stresses or were significantly differentially expressed among multiple tissues were screened as functional gene resources.SlPLATZ17 was selected for functional verification by experiment-based analysis.The results showed that the downregulation of SlPLATZ17 gene expression reduced the drought and salt tolerance of tomato plants.Tomato plants overexpressing SlPLATZ17 had larger flower sizes and long,thin petals,adjacent petals were not connected at the base,and the stamen circumference was smaller.This study contributes to understanding the functions of the SlPLATZ family in tomato and provides a reference for functional gene screening.
基金supported by the National Natural Science Foundation of China(31871624)the Agricultural Science and Technology Innovation Program(CAAS-ZDRW202109 and CAAS-ZDRW202002)the Central Public-interest Scientific Institution Basal Research Fund.
文摘Plant AT-rich sequence and zinc binding(PLATZ)transcription factors are a class of plant specific zincdependent DNA-binding proteins that function in abiotic stress response and plant development.In this study,31 GmPLATZ genes were identified in soybean.GmPLATZ17 was down-regulated by drought and exogenous abscisic acid.Transgenic Arabidopsis and soybean hairy roots overexpressing GmPLATZ17 showed drought sensitivity and inhibition of stress-associated gene transcription.In contrast,suppressed expression of GmPLATZ17 led to increased drought tolerance in transgenic soybean hairy roots.The GmPLATZ17 protein was verified to interact physically with the GmDREB5 transcription factor,and overexpression of GmDREB5 increased drought tolerance in soybean hairy roots.Interaction of GmPLATZ17 with GmDREB5 was shown to interfere with the DRE-binding activity of GmDREB5,suppressing downstream stress-associated gene expression.These results show that GmPLATZ17 inhibits drought tolerance by interacting with GmDREB5.This study sheds light on PLATZ transcription factors and the function of GmPLATZ17 in regulating drought sensitivity.
基金This work was supported by the“National Key R&D Program of China(2018YFD0200500)”“Open Project Program of Engineering Research Center of Ecology and Agricultural Use of Wetland,Ministry of Education(KF201802)”“Open Project Program of Shanxi Key Laboratory of Integrated Pest Management in Agriculture,Institute of Plant Protection(YHSW2018002)”.
文摘Plant AT-rich sequence and zinc-binding protein(PLATZ)is a plant transcription factor that has been studied in corn.PLATZ can non-specifically bind to sequences rich in A/T bases to induce transcriptional repression.It is involved in the regulation of dehydration tolerance in seeds.In this study,we performed bioinformatics analysis to identify and characterize wheat PLATZ(TaPLATZ)genes.We identified 49 wheat PLATZ genes by searching the wheat genome by using known PLATZ gene sequences from rice,Arabidopsis,and maize.Phylogenetic analysis on PLATZ gene sequences from different species was performed.We found that PLATZs could be divided into three groups.The chromosome(chr)distribution analysis revealed that the 49 identified wheat PLATZ genes are distributed in 15 chrs.Gene structure and motif analyses indicated that most PLATZ genes possess conserved exon/intron arrangements and motif compositions.Our analysis of transcriptional data indicated that several wheat PLATZ genes may play an important role in abiotic stress resistance given that they are expressed under salt stress.The results of qRT-PCR further confirmed that TaPLATZ is involved in plant abiotic stress and is also related to the cell differentiation of plant tissues.Our results lay the foundation for further studies on the function of the wheat PLATZ gene family.
基金supported by grants from the National Natural Science Foundation of China(32100278)the China Postdoctoral Science Foundation(2023M741225)the Postdoctoral Fellowship Program(Grade C)of China Postdoctoral Science Foundation(GZC20230864)。
文摘Grain size is an important agronomic trait that largely determines grain yield in rice(Oryza sativa L.).The genes encoding the Growth Regulating Factors(GRFs)and G-proteins are major regulators for grain length regulation,but how these pathways are coordinated in plants remains elusive.Here,we described OsPLATZ1 as a transcriptional activator,a member of the Plant AT-rich sequence-and Zinc-binding family proteins in rice that positively regulates grain length.OsPLATZ1 interacted with multiple GRFs,and the OsPLATZ1-Os GRF4 complex bound to regulatory regions in the promoter of the G-protein gene DENSE AND ERECT PANICLE1(DEP1)to enhance its expression,thereby regulating grain length.We used gene editing to modify the OsPLATZ1 promoter regulatory region and obtained mutant lines with downregulated or upregulated OsPLATZ1 expression depending on the type of editing event.One of these mutant lines had changes in multiple agronomic traits and improved grain yield and grain appearance quality.Our findings reveal a new regulatory module in which OsPLATZ1 connects the GRFs and G-protein signaling pathways to regulate grain length and suggest that finely modulating OsPLATZ1activity might be a promising molecular breeding approach.
基金supported by grants from the Agricultural Variety Improvement Project of Shandong Province(2022LZGC002)the National Natural Science Foundation of China(32171935 and 32372039)+2 种基金the National Key Research and Development Program of China(2022YFD1201700)the Natural Science Foundation of Shandong Province(ZR2019ZD16)the Intramural Joint Program Fund of State Key Laboratory of Microbial Technology(SKLMTIJP2024-06)。
文摘Saline-alkaline soils are a major environmental problem that limit plant growth and crop productivity.Plasma membrane H^(+)-ATPases and the salt overly sensitive(SOS)signaling pathway play important roles in plant responses to saline-alkali stress.However,little is known about the functional genes and mechanisms regulating the transcription of H^(+)-ATPases and SOS pathway genes under saline–alkali stress.In the present study,we identified that the plant AT-rich sequence and zinc-binding(TaPLATZ2)transcription factor are involved in wheat response to saline-alkali stress by directly suppressing the expression of TaHA2/TaSOS3.The knockdown of TaPLATZ2 enhances salt and alkali stress tolerance,while overexpression of TaPLATZ2 leads to salt and alkali stress sensitivity in wheat.In addition,TaWRKY55 directly upregulated the expression of TaPLATZ2 during saline-alkali stress.Through knockdown and overexpression of Ta WRKY55 in wheat,Ta WRKY55 was shown to negatively modulate salt and alkali stress tolerance.Genetic analyses confirmed that Ta PLATZ2 functions downstream of Ta WRKY55 in response to salt and alkaline stresses.These findings provide a TaWRKY55–TaPLATZ2–TaHA2/TaSOS3 regulatory module that regulates wheat responses to saline-alkali stress.
