摘要
PLATZ是一类植物特异性的锌指类转录因子(plant AT-rich sequence and zinc-binding),在植物的生长发育和抗逆中发挥重要作用。为了更好地解析茶树[Camellia sinensis(L.)Kuntze]PLATZ基因,明确其在干旱及高温条件下的响应,本研究对茶树品种‘铁观音’PLATZ基因家族进行了系统鉴定,共鉴定到12个CsPLATZ家族成员。利用Expasy在线软件及其他生物信息学软件对PLATZ基因家族成员进行蛋白质理化性质、进化关系、顺式作用元件、基因结构、物种内及物种间共线性等分析。进化分析结果将CsPLATZ家族分为2个亚家族;保守结构域分析结果表明,同一亚家族内的PLATZ转录因子家族成员的保守结构域种类及基因结构具有高度一致性,而不同亚家族之间则呈现一定的多样性。12个PLATZ基因不均匀分布在茶树的7条染色体上,这些基因的启动子区域具有多种与激素及逆境胁迫响应有关的顺式作用元件;共线性分析表明,CsPLATZ基因家族存在4对重复事件,均为片段复制,与水稻(Oryza sativa L.)相比,茶树和拟南芥[Arabidopsis thaliana(L.)Heynh.]之间存在更近的进化关系;转录组分析显示,CsPLATZ家族成员在茶树不同组织部位的表达存在差异。挑选在芽、幼叶和根部表达量较高的6个基因(CsPLATZ-1、CsPLATZ-2、CsPLATZ-3、CsPLATZ-4、CsPLATZ-6、CsPLATZ-8)分别进行高温、干旱胁迫处理,利用实时荧光定量PCR(quantitative real-time polymerase chain reaction,qRT-PCR)对其表达进行定量分析。结果表明,CsPLATZ-1、CsPLATZ-2、CsPLATZ-3、CsPLATZ-4、CsPLATZ-6、CsPLATZ-8可能在茶树响应干旱胁迫中发挥重要作用;CsPLATZ-2、CsPLATZ-8可能在高温胁迫响应中具有重要功能。本研究结果有助于更好地理解PLATZ基因的生物学功能及其在茶树生长发育及抗逆过程中可能发挥的作用。
The plant AT-rich sequence and zinc-binding protein(PLATZ)family is composed of plant-specific zinc finger-like transcription factors,which play important roles in plant growth,development,and stress tolerance.In this study,to gain a better understanding of the PLATZ gene in C.sinensis and elucidate its response under drought and high temperature conditions,the PLATZ gene family of the C.sinensis cultivar‘Tieguanyin’was systematically identified,and a total of 12 CsPLATZ family members were identified.Expasy online and other bioinformatics tools were used to analyze the members of the PLATZ gene family in terms of protein physicochemical properties,phylogenetic relationships,cis-acting elements,gene structures,and intra-and inter-species collinearity.The results of phylogenetic analysis classified the CsPLATZ family members into 2 subfamilies.The conserved domains and gene structures of PLATZ family members within the same subfamily had a high degree of consistency,whereas a certain degree of diversity was observed among the subfamilies.Twelve PLATZ genes were unevenly distributed across 7 chromosomes of C.sinensis and the promoter regions of these genes had multiple cis-acting elements related to hormone and stress responses.The collinearity analysis showed that there were 4 pairs of duplication events in the CsPLATZ gene family,all of which were segmental duplications.Based on this gene family,C.sinensis had a closer evolutionary relationship with A.thaliana than with O.sativa.The transcriptome analysis showed that the expression levels of CsPLATZ family members varied in different tissue samples of C.sinensis.6 genes(CsPLATZ-1,CsPLATZ-2,CsPLATZ-3,CsPLATZ-4,CsPLATZ-6,and CsPLATZ-8)with high expression in shoots,young leaves,and roots were selected for high temperature and drought stress treatments,and their expression was quantified by qRT-PCR.The results indicated that the six genes might play important roles in the response to drought stress.In addition,CsPLATZ-2 and CsPLATZ-8 might have important functions in the response to high temperature stress.The results of this study will contribute to a better understanding of the biological functions of PLATZ genes and their possible roles in the growth,development,and stress responses of C.sinensis.
作者
易晓姝
郑安儒
周承哲
田采云
张成
郭玉琼
陈暄
YI Xiaoshu;ZHENG Anru;ZHOU Chengzhe;TIAN Caiyun;ZHANG Cheng;GUO Yuqiong;CHEN Xuan(College of Horticulture,Nanjing Agricultural University,Nanjing 210095,Jiangsu,China;Anxi College of Tea Science/College of Horticulture,Fujian Agriculture and Forestry University,Fuzhou 350002,Fujian,China)
出处
《生物工程学报》
北大核心
2025年第7期2897-2912,共16页
Chinese Journal of Biotechnology
基金
江苏省重点研发计划(BE2023364)。
关键词
PLATZ基因家族
茶树
生物信息学分析
高温
干旱
PLATZ gene family
Camellia sinensis
bioinformatics analysis
high temperature
drought
