The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cell...The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E2) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E2, PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.展开更多
we will demonstrate the ability of multiple and specific alternative medical technologies used in combination that support the body’s natural ability to repair peripheral nerves in neuropathy patients. This paper loo...we will demonstrate the ability of multiple and specific alternative medical technologies used in combination that support the body’s natural ability to repair peripheral nerves in neuropathy patients. This paper looks to review currently known biological foundations for the regenerative effects of Low-Level Laser Therapy, Pulsed Electromagnetic Field Therapy, Infrared and Near Infrared Therapy<span style="font-family:;" "="">,</span><span style="font-family:;" "=""> and Nutritional Protocols to demonstrate the significant potential to repair peripheral nerve injuries. There is favorable evidence to expand functional recovery in diverse types of peripheral nerve injuries. The conclusion from this study indicates using a specific combination of technologies that scientifically enhances the body’s ability to repair nerves is more effective in helping patients with neuropathies than the normal standard of care.</span>展开更多
The interaction of pulsed electromagnetic fields (PEMF) with the human body may result in a variety of positive outcomes including analgesia, enhanced healing, chondroprotection, cognitive improvement and better quali...The interaction of pulsed electromagnetic fields (PEMF) with the human body may result in a variety of positive outcomes including analgesia, enhanced healing, chondroprotection, cognitive improvement and better quality of life. Previous human studies have also revealed the potential of PEMF to enhance muscle function and athletic performance. To further evaluate this potential, an open label pilot study was conducted with 19 competitive cyclists who repeatedly participated in 63 training routes. Cyclist performance was tracked before and during use of a novel and portable PEMF device that is worn as a wristband. Comparison of performance before and during use of the wristband revealed a significant association with improved muscle power. The odds ratio was 3.02 (P < 0.01) for experiencing increased muscle power while wearing the PEMF device. Among the cycling routes in which an increase was observed, the average increase in power was about 9.8%. The data suggests the novel PEMF technology may be a safe and effective therapeutic approach for improved physical performance and likely involves improved oxygen delivery due to reduced rouleaux (erythrocyte aggregation). These results warrant further investigation comprising larger studies and additional outcomes.展开更多
Nerve regeneration following traumas remains an unmet challenge.The application of pulsed electromagnetic field(PEMF)stimulation has gained traction for a minimally invasive regeneration of nerves.However,a sys-temati...Nerve regeneration following traumas remains an unmet challenge.The application of pulsed electromagnetic field(PEMF)stimulation has gained traction for a minimally invasive regeneration of nerves.However,a sys-tematic exploration of different PEMF parameters influencing neuron function at a cellular level is not available.In this study,we exposed neuroblastoma F11 cells to PEMF to trigger beneficial effects on neurite outgrowth.Different carrier frequencies,pulse repetition frequencies,and duty cycles were screened with a custom ad hoc setup to find the most influential parameters values.A carrier frequency of 13.5 MHz,a pulse repetition frequency of 20 Hz,and a duty cycle of 10%allowed maximal neurite outgrowth,with unaltered viability with respect to non-stimulated controls.Furthermore,in a longer-term analysis,such optimal conditions were also able to in-crease the gene expression of neuronal expression markers NeuN and Tuj-1 and transcription factor Ngn1.Finally,the same optimal stimulation conditions were also applied to THP-1 macrophages,and both pro-inflammatory(TNF-α,IL-1β,IL-6,IL-8)and anti-inflammatory cytokines(IL-10,CD206)were analyzed.The optimal PEMF stimulation parameters did not induce differentiation towards an M1 macrophage phenotype,decreased IL-1β and IL-8 gene expression,decreased TNF-α and IL-8 cytokine release in M1-differentiated cells,increased IL-10 and CD206 gene expression,as well as IL-10 cytokine release in M0 cells.The specific PEMF stimulation regime,which is optimal in vitro,might have a high potential for a future in vivo translation targeting neural regeneration and anti-inflammatory action for treating peripheral nerve injuries.展开更多
文摘The study investigated the effects of pulsed electromagnetic fields (PEMFs) of different frequencies on the gene expression of receptor activator of nuclear factor kappa B (RANK) and Nuclear factor of activated T-cells cytoplasmic 1 (NFATc1) in rat osteoblast and osteoclast co-cultured model. Osteoblast-like cells were isolated from calvariae of Newborn Sprague Dawley rats (SD rats), while osteoclast-like cells were obtained from femora and tibiae of five weeks old SD rats. After 1 days of co-culture, the cells were exposed to premarin (E2) and different frequencies of PEMFs (8 Hz and 16 Hz, respectively) for 3 days. The expression of RANK and NFATc1 mRNA was analysed with realtime quantitative polymerase chain reaction. The gene expression of RANK and NFATc1 in the E2, PEMF with 8 Hz and 16 Hz group was significantly lower than that in the control group respectively. The gene expression of NFATc1 in the PEMF with 8 Hz group was significantly lower than that in the control group and PEMF with 16 Hz group. The study indicates that PEMF with 8 Hz could regulate the gene expression of RANK and NFATc1 in co-cultured model.
文摘we will demonstrate the ability of multiple and specific alternative medical technologies used in combination that support the body’s natural ability to repair peripheral nerves in neuropathy patients. This paper looks to review currently known biological foundations for the regenerative effects of Low-Level Laser Therapy, Pulsed Electromagnetic Field Therapy, Infrared and Near Infrared Therapy<span style="font-family:;" "="">,</span><span style="font-family:;" "=""> and Nutritional Protocols to demonstrate the significant potential to repair peripheral nerve injuries. There is favorable evidence to expand functional recovery in diverse types of peripheral nerve injuries. The conclusion from this study indicates using a specific combination of technologies that scientifically enhances the body’s ability to repair nerves is more effective in helping patients with neuropathies than the normal standard of care.</span>
文摘The interaction of pulsed electromagnetic fields (PEMF) with the human body may result in a variety of positive outcomes including analgesia, enhanced healing, chondroprotection, cognitive improvement and better quality of life. Previous human studies have also revealed the potential of PEMF to enhance muscle function and athletic performance. To further evaluate this potential, an open label pilot study was conducted with 19 competitive cyclists who repeatedly participated in 63 training routes. Cyclist performance was tracked before and during use of a novel and portable PEMF device that is worn as a wristband. Comparison of performance before and during use of the wristband revealed a significant association with improved muscle power. The odds ratio was 3.02 (P < 0.01) for experiencing increased muscle power while wearing the PEMF device. Among the cycling routes in which an increase was observed, the average increase in power was about 9.8%. The data suggests the novel PEMF technology may be a safe and effective therapeutic approach for improved physical performance and likely involves improved oxygen delivery due to reduced rouleaux (erythrocyte aggregation). These results warrant further investigation comprising larger studies and additional outcomes.
文摘Nerve regeneration following traumas remains an unmet challenge.The application of pulsed electromagnetic field(PEMF)stimulation has gained traction for a minimally invasive regeneration of nerves.However,a sys-tematic exploration of different PEMF parameters influencing neuron function at a cellular level is not available.In this study,we exposed neuroblastoma F11 cells to PEMF to trigger beneficial effects on neurite outgrowth.Different carrier frequencies,pulse repetition frequencies,and duty cycles were screened with a custom ad hoc setup to find the most influential parameters values.A carrier frequency of 13.5 MHz,a pulse repetition frequency of 20 Hz,and a duty cycle of 10%allowed maximal neurite outgrowth,with unaltered viability with respect to non-stimulated controls.Furthermore,in a longer-term analysis,such optimal conditions were also able to in-crease the gene expression of neuronal expression markers NeuN and Tuj-1 and transcription factor Ngn1.Finally,the same optimal stimulation conditions were also applied to THP-1 macrophages,and both pro-inflammatory(TNF-α,IL-1β,IL-6,IL-8)and anti-inflammatory cytokines(IL-10,CD206)were analyzed.The optimal PEMF stimulation parameters did not induce differentiation towards an M1 macrophage phenotype,decreased IL-1β and IL-8 gene expression,decreased TNF-α and IL-8 cytokine release in M1-differentiated cells,increased IL-10 and CD206 gene expression,as well as IL-10 cytokine release in M0 cells.The specific PEMF stimulation regime,which is optimal in vitro,might have a high potential for a future in vivo translation targeting neural regeneration and anti-inflammatory action for treating peripheral nerve injuries.
