摘要
目的 应用脉冲电磁场 (PEMFs)刺激体外分离培养的人骨髓间充质干细胞 (hMSCs) ,对其生物学特性进行研究。方法 PEMFs (1 2Hz、 1 1mT、 8h/d)刺激hMSCs体外培养 ,计算细胞的倍增时间 ,观察细胞超微结构 ,放免法测定骨钙素的分泌情况和钙化结节VonKossa染色。结果 脉冲电磁场作用后的细胞增殖能力提高 ,细胞形态发生变化 ,透射电镜观察提示细胞成熟 ,VonKossa钙染色阳性 ,骨钙素的分泌活性增高。结论 hMSCs经合理的PEMFs体外刺激培养后 ,不仅能够大量扩增细胞 ,同时能够向成骨细胞转化 ,具有一定的成骨活性 。
Objective To study biological characteristics of cultured human mesenchymal stem cells in vitro with pulsed electromagnetic fields(PEMFs) stimulated.Methods After hMSCs were stimulated by the PEMFs(12Hz pulse burst,1.1mT peak,8h/day),measure the cellular doubling timeon、osteocalcin concentration,observe cells ultrastructure and Von kossa staining for calcium nodule were applied to measure the hMSCs differentiation.Results PEMFs treatment accelerated cellar proliferation obviously,At the same time the cellular form was changed,A transmission electron micrograph of The third passaged MSCs cultured with PEMFs stimuated showing expanded endoplasmic reticulum,staining for calcium nodule were positive,the osteocalcin concentration was also increased.Conclusion After reasonable PEMFs stimulating the hMSCs,cellar proliferation and cellar differentiation can be accelerated.This study reveals that the hMSCs could be used as the seed cells in the bone tissue engineering.
出处
《骨与关节损伤杂志》
2004年第6期387-389,共3页
The Journal of Bone and Joint Injury
基金
国家 8 63计划重大专项 (编号 :2 0 0 3AA2 0 50 0 2 )
重庆市重点科技攻关基金资助项目 (编号 :2 0 0 3 - 1 3 - 6)
