The localization and distribution of mRNAs and antigens of tissue type(t)and urokinasetype(u)plasminogen activator(PA)plus their corresponding inhibitors type-1(PAI-1)and type-2(PAI-2)were studied in human and rhesus ...The localization and distribution of mRNAs and antigens of tissue type(t)and urokinasetype(u)plasminogen activator(PA)plus their corresponding inhibitors type-1(PAI-1)and type-2(PAI-2)were studied in human and rhesus monkey placentae by in situ hybridization andimmunofluorencence We haye demonstrated that(1)plasmjnogen activators(tPA and uPA),and theirinhibitors(PAl-1 and PAl-2)for both mRNAs and proteins in most cases,co-localized fn the same areas.hut with different extent,In human and rhesus menkey placentae:(2)All the molecules,tPA.uPA.PAI-1,and PAl-2 and their mRNAs were identified in the wall of blood vessels of villi stem and decidua,extravillous cytotrophoblast cells of basal plate between Rohr's and Nitabuch's striace,trophoblast shelland decidual gland cells as well as eytotrophoblast cells of villi.However,tPA and PAI-1 were found tobe more evident in the regions adjacent to abscission planes from the maternal tissue,while uPA andPA1-2 concentrated mainly in villous trophoblast and extravillous trophoblast cells;(3)Distribution ofthe mRNAs of the 4 genes is generally consistent with the immunofluorescent pattern of thecorresponding antigens.however,no expressien of the mRNAs.but their proteins w'as observed insyncytiotrophoblast cells of villi;(4)The patterm of distribution and synthesis of the aetivators andinhibitors coincides with the distribution of fibrin-like molecules:(5)The distribution pattern of themRVAs and their proteins was similar in monkey and human placentae.no spcies-difference wasdemonstrated.The data suggest that the activators and the inhibitors described here interact at avariety of crucial physiological stages of pregnancy.They may interact in tightly restricted locations tothe matrix fibrinoids in rather short ranges and at specifie times,particularly during early and latepregnaney,to promote healthy gestation.It is possible that failure of the local control over the systemmay be important in failure of implantation and premature rupture of membranes.We new proposethat the location of these molecules gives the system potential in the control over establishment of utero-fetal blood circulation and placentatiton in the first two trimesters of gestation and abscission during thethird stage of labour,and predict a role in the pathology of abrupie placenta and retained placenta)products.展开更多
基金supported by WHO/Rockefeller Foundaton(RF9602078)the Welcome Trust(058659/2/99/2)+2 种基金the Royal Society of UK(RL/ART/CAN/JPQ7681)National Natural Science Foundationof China(39911130503)Chinese Academy of Seiences(KJ952-J1-603).
文摘The localization and distribution of mRNAs and antigens of tissue type(t)and urokinasetype(u)plasminogen activator(PA)plus their corresponding inhibitors type-1(PAI-1)and type-2(PAI-2)were studied in human and rhesus monkey placentae by in situ hybridization andimmunofluorencence We haye demonstrated that(1)plasmjnogen activators(tPA and uPA),and theirinhibitors(PAl-1 and PAl-2)for both mRNAs and proteins in most cases,co-localized fn the same areas.hut with different extent,In human and rhesus menkey placentae:(2)All the molecules,tPA.uPA.PAI-1,and PAl-2 and their mRNAs were identified in the wall of blood vessels of villi stem and decidua,extravillous cytotrophoblast cells of basal plate between Rohr's and Nitabuch's striace,trophoblast shelland decidual gland cells as well as eytotrophoblast cells of villi.However,tPA and PAI-1 were found tobe more evident in the regions adjacent to abscission planes from the maternal tissue,while uPA andPA1-2 concentrated mainly in villous trophoblast and extravillous trophoblast cells;(3)Distribution ofthe mRNAs of the 4 genes is generally consistent with the immunofluorescent pattern of thecorresponding antigens.however,no expressien of the mRNAs.but their proteins w'as observed insyncytiotrophoblast cells of villi;(4)The patterm of distribution and synthesis of the aetivators andinhibitors coincides with the distribution of fibrin-like molecules:(5)The distribution pattern of themRVAs and their proteins was similar in monkey and human placentae.no spcies-difference wasdemonstrated.The data suggest that the activators and the inhibitors described here interact at avariety of crucial physiological stages of pregnancy.They may interact in tightly restricted locations tothe matrix fibrinoids in rather short ranges and at specifie times,particularly during early and latepregnaney,to promote healthy gestation.It is possible that failure of the local control over the systemmay be important in failure of implantation and premature rupture of membranes.We new proposethat the location of these molecules gives the system potential in the control over establishment of utero-fetal blood circulation and placentatiton in the first two trimesters of gestation and abscission during thethird stage of labour,and predict a role in the pathology of abrupie placenta and retained placenta)products.
