摘要
目的 研究兔角膜碱烧伤后修复过程中 ,组织型纤溶酶原激活剂 (tPA)和 2型纤溶酶原激活剂抑制剂 (PAI 2 )的作用。方法 在构建离体和在体兔角膜碱烧伤模型的基础上 ,采用细胞培养、组织切片、免疫细胞化学以及图象分析等技术。结果 tPA的表达在碱烧伤后 2 4小时左右达到峰值 ,而PAI 2则在伤后4 8小时表达最高 ,离体表达部位位于胞浆 ,并相对靠近胞膜和核膜 ,在体表达部位主要位于角膜上皮层。结论 tPA和PAI
Objective To explore the function of tPA and PAI-2 in the course of corneal repair after alkali burn. Methods After the models of alkali burn of cornea were established successfully in vivo and in vitro,some techniques such as cell culture,tissue slice,ICC and image analysis were used. Results The expression of tPA increased to the maximum at about 24 hours after alkali burn,whereas the maximum expression of PAI-2 was at located relatively about 48 hours. The part of their expression in vivo distributed in the cytoplasm which was nearer to the membrane of cell and nucleus. In vitro it was mainly distributed in the corneal epithelial layer. Conclusion General characters may exist in the function of tPA and PAI-2 in epithelial tissue.
出处
《创伤外科杂志》
2003年第4期282-285,共4页
Journal of Traumatic Surgery
基金
国家重点基础研究发展规划项目 (G1 9990 5 4 2 0 4 )
