目的:黏蛋白1(mucin 1,MUC1)在胰腺癌(pancreatic adenocarcinoma,PAAD)中的表达与患者预后的关联存在争议,部分研究显示其高表达与不良预后相关。叉状头转录因子P3(forkhead box protein 3,FOXP3)作为调节性T细胞(regulatory T cells,T...目的:黏蛋白1(mucin 1,MUC1)在胰腺癌(pancreatic adenocarcinoma,PAAD)中的表达与患者预后的关联存在争议,部分研究显示其高表达与不良预后相关。叉状头转录因子P3(forkhead box protein 3,FOXP3)作为调节性T细胞(regulatory T cells,Tregs)的特异性标志物,在PAAD肿瘤细胞、肿瘤微环境(tumor microenvironment,TME)及癌旁胰腺组织中均有表达,但其与MUC1的潜在关联及对患者预后的提示作用尚不明确。本研究旨在探讨PAAD组织中MUC1和FOXP3的表达水平与临床病理特征的关系,以及其对患者生存预后的影响。方法:收集山西医科大学附属山西省人民医院2018年1月至2024年6月收治的28例PAAD患者手术切除标本的癌组织及癌旁组织,采用免疫组织化学法检测MUC1、FOXP3的表达,分析MUC1和FOXP3表达与PAAD患者临床病理特征及总生存期(overall survival,OS)的关系。结果:PAAD癌组织中MUC1阳性表达率高于癌旁组织(P<0.001);癌组织中MUC1阳性患者术前血清总胆红素水平较阴性患者高(W=41.000,P=0.008);癌组织中MUC1表达水平与术前血清总胆红素水平呈正相关(r=0.456,P=0.015)。癌组织中FOXP3蛋白阳性表达率低于癌旁组织(P=0.011);癌组织中FOXP3阳性患者术前血清淋巴细胞计数低于阴性患者(W=101.000,P=0.010),且肿瘤最大径较小(W=108.000,P=0.002);癌组织中FOXP3表达水平与术前血清淋巴细胞计数(r=−0.443,P=0.018)、肿瘤最大径(r=−0.773,P<0.001)呈负相关。癌组织MUC1与FOXP3表达无关(r=0.025,P=0.898)。Kaplan-Meier分析表明癌组织中MUC1和FOXP3阳性表达均不提示预后不良(均P>0.05),而癌旁组织中FOXP3阳性表达提示患者预后不良(AUC=0.641,χ^(2)=5.398,P=0.020)。结论:PAAD癌组织中MUC1表达与患者术前血清总胆红素水平呈正相关;癌组织中FOXP3表达与患者术前血清淋巴细胞计数、肿瘤最大径呈负相关。癌组织中MUC1和FOXP3表达无关联且均不提示预后不良,癌旁组织中FOXP3阳性表达可提示患者预后不良。展开更多
Background:Growth differentiation factor 11(GDF11),a transforming growth factor-beta superfamily member,is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis,and it has bee...Background:Growth differentiation factor 11(GDF11),a transforming growth factor-beta superfamily member,is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis,and it has been extensively characterized due to its capacity to target poorly differentiated cells,including transformed or cancer cells.Aim:In the present work,we aimed to describe the effects on migration,proliferation,and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.Methods:Based on previous evidence,we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.Results:We found a profound impact on mitochondrial metabolism and reactive oxygen species content;these were related to a decrement in the expression of the transcription factor forkhead-box-protein P3(FOXP3),which is highly involved in aggressiveness in leukemia cells;this was verified by a decrement in invasion capacity exhibited by the Jurkat cells under the GDF11 treatment.Conclusion:The results position the GDF11 response as a good alternative in the search for new therapeutic options for these diseases.展开更多
文摘目的:黏蛋白1(mucin 1,MUC1)在胰腺癌(pancreatic adenocarcinoma,PAAD)中的表达与患者预后的关联存在争议,部分研究显示其高表达与不良预后相关。叉状头转录因子P3(forkhead box protein 3,FOXP3)作为调节性T细胞(regulatory T cells,Tregs)的特异性标志物,在PAAD肿瘤细胞、肿瘤微环境(tumor microenvironment,TME)及癌旁胰腺组织中均有表达,但其与MUC1的潜在关联及对患者预后的提示作用尚不明确。本研究旨在探讨PAAD组织中MUC1和FOXP3的表达水平与临床病理特征的关系,以及其对患者生存预后的影响。方法:收集山西医科大学附属山西省人民医院2018年1月至2024年6月收治的28例PAAD患者手术切除标本的癌组织及癌旁组织,采用免疫组织化学法检测MUC1、FOXP3的表达,分析MUC1和FOXP3表达与PAAD患者临床病理特征及总生存期(overall survival,OS)的关系。结果:PAAD癌组织中MUC1阳性表达率高于癌旁组织(P<0.001);癌组织中MUC1阳性患者术前血清总胆红素水平较阴性患者高(W=41.000,P=0.008);癌组织中MUC1表达水平与术前血清总胆红素水平呈正相关(r=0.456,P=0.015)。癌组织中FOXP3蛋白阳性表达率低于癌旁组织(P=0.011);癌组织中FOXP3阳性患者术前血清淋巴细胞计数低于阴性患者(W=101.000,P=0.010),且肿瘤最大径较小(W=108.000,P=0.002);癌组织中FOXP3表达水平与术前血清淋巴细胞计数(r=−0.443,P=0.018)、肿瘤最大径(r=−0.773,P<0.001)呈负相关。癌组织MUC1与FOXP3表达无关(r=0.025,P=0.898)。Kaplan-Meier分析表明癌组织中MUC1和FOXP3阳性表达均不提示预后不良(均P>0.05),而癌旁组织中FOXP3阳性表达提示患者预后不良(AUC=0.641,χ^(2)=5.398,P=0.020)。结论:PAAD癌组织中MUC1表达与患者术前血清总胆红素水平呈正相关;癌组织中FOXP3表达与患者术前血清淋巴细胞计数、肿瘤最大径呈负相关。癌组织中MUC1和FOXP3表达无关联且均不提示预后不良,癌旁组织中FOXP3阳性表达可提示患者预后不良。
基金funded by a grant from the Consejo Nacional de humanidades Ciencia y Tecnología(Conahcyt).Fronteras de la Ciencia 1320,Infra-2017280788Universidad Autónoma Metropolitana-Iztapalapa.
文摘Background:Growth differentiation factor 11(GDF11),a transforming growth factor-beta superfamily member,is a crucial protein involved in many differentiation processes in embryogenesis and morphogenesis,and it has been extensively characterized due to its capacity to target poorly differentiated cells,including transformed or cancer cells.Aim:In the present work,we aimed to describe the effects on migration,proliferation,and metabolism in the T-cell acute lymphoblastic leukemia-derived cell line Jurkat.Methods:Based on previous evidence,we analyzed metabolic changes exerted by GDF11 and its relationship with the aggressive phenotype.Results:We found a profound impact on mitochondrial metabolism and reactive oxygen species content;these were related to a decrement in the expression of the transcription factor forkhead-box-protein P3(FOXP3),which is highly involved in aggressiveness in leukemia cells;this was verified by a decrement in invasion capacity exhibited by the Jurkat cells under the GDF11 treatment.Conclusion:The results position the GDF11 response as a good alternative in the search for new therapeutic options for these diseases.
