We genotyped 23 Y-STR loci（DYS576, DYS389 I, DYS448, DYS389 II, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643, DYS393, DYS458, DYS385a/b, DYS456, and GATA-H4） ...We genotyped 23 Y-STR loci（DYS576, DYS389 I, DYS448, DYS389 II, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643, DYS393, DYS458, DYS385a/b, DYS456, and GATA-H4） in a sample of 109 unrelated male Chinese people residing in Minnan area and compared the results with those from our previous study on 12 Y-STR. The haplotype diversity and the discrimination capacity of the 23 Y-STR reached 0.9903 and 0.9725, respectively, and the genetic diversity for each locus ranged from 0.321（DYS391） to 0.955（DYS385）. Besides, we observed a strong correlation between the number of Y-STR markers and the substantial improvement of forensic parameters used to discriminate between individuals. The results indicated that these highly polymorphic Y-STR markers were useful for human identification in forensic cases and paternity tests within the Minnan Han Chinese population.展开更多
目的对D19S433基因座稀有等位基因8.2,用分子生物学方法,验证其命名,对突变发生的位置进行确认和分析。方法设计引物对目的基因进行扩增和测序,验证常规命名法。将测序所得序列与D19S433基因座的基础序列进行比对分析。结果Goldeneye DN...目的对D19S433基因座稀有等位基因8.2,用分子生物学方法,验证其命名,对突变发生的位置进行确认和分析。方法设计引物对目的基因进行扩增和测序,验证常规命名法。将测序所得序列与D19S433基因座的基础序列进行比对分析。结果Goldeneye DNA 20A和AGCU EX22亲子鉴定系统联合检测,相互比对,确定在检案中发现的分型标准物之外(Off⁃ladder,OL)的等位基因为D19S433基因座的稀有等位基因。经常规漂移校正计算该等位基因为8.2。测序后分析其重复序列确定该等位基因为8.2无误。结论对STR分型中发现的稀有等位基因进行测序,分析其重复序列,可以准确的对其进行命名,确定稀有等位基因突变的位置,丰富中国人群STR数据信息。展开更多
基金supported by grants from the Li Ka-Shing Foundation(Hong Kong,China),Cambridge University,UK(No.432034)the Key Research Project of Shantou Science and Technology Bureau(No.2005116)the Shantou University Research and Development Fund(No.L00007)
文摘We genotyped 23 Y-STR loci（DYS576, DYS389 I, DYS448, DYS389 II, DYS19, DYS391, DYS481, DYS549, DYS533, DYS438, DYS437, DYS570, DYS635, DYS390, DYS439, DYS392, DYS643, DYS393, DYS458, DYS385a/b, DYS456, and GATA-H4） in a sample of 109 unrelated male Chinese people residing in Minnan area and compared the results with those from our previous study on 12 Y-STR. The haplotype diversity and the discrimination capacity of the 23 Y-STR reached 0.9903 and 0.9725, respectively, and the genetic diversity for each locus ranged from 0.321（DYS391） to 0.955（DYS385）. Besides, we observed a strong correlation between the number of Y-STR markers and the substantial improvement of forensic parameters used to discriminate between individuals. The results indicated that these highly polymorphic Y-STR markers were useful for human identification in forensic cases and paternity tests within the Minnan Han Chinese population.
文摘目的对D19S433基因座稀有等位基因8.2,用分子生物学方法,验证其命名,对突变发生的位置进行确认和分析。方法设计引物对目的基因进行扩增和测序,验证常规命名法。将测序所得序列与D19S433基因座的基础序列进行比对分析。结果Goldeneye DNA 20A和AGCU EX22亲子鉴定系统联合检测,相互比对,确定在检案中发现的分型标准物之外(Off⁃ladder,OL)的等位基因为D19S433基因座的稀有等位基因。经常规漂移校正计算该等位基因为8.2。测序后分析其重复序列确定该等位基因为8.2无误。结论对STR分型中发现的稀有等位基因进行测序,分析其重复序列,可以准确的对其进行命名,确定稀有等位基因突变的位置,丰富中国人群STR数据信息。
