[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of ...[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of total flavonoids in C.tangutorum O.E.Schulz was optimized by Box-Behnken design from aspects of ethanol volume fraction,extraction time,extraction temperature and enzyme addition,with yield of total flavonoids as the evaluation index.[Results]The optimal extraction process of total flavonoids in C.tangutorum O.E.Schulz was as follows:ethanol volume fraction of 70%,ultrasonic extraction time of 43 min,ultrasonic extraction temperature of 37℃and cellulose addition of 0.07 mg.Under the optimal conditions,the yield of total flavonoids from C.tangutorum O.E.Schulz reached 75.90 mg/g.[Conclusions]The optimized extraction process was reasonable and feasible,and it could provide a reference for the extraction of total flavonoids in C.tangutorum O.E.Schulz.This study provided a foundation for the quality control of food/medicinal materials and the development and research of big health products of C.tangutorum O.E.Schulz.展开更多
[Objectives]This study aimed to determine the contents of inorganic elements in Cardamine tangutorum O.E.Schulz samples from different origins to provide a scientific reference for the quality control,safety evaluatio...[Objectives]This study aimed to determine the contents of inorganic elements in Cardamine tangutorum O.E.Schulz samples from different origins to provide a scientific reference for the quality control,safety evaluation and clinical medicinal use of C.tangutorum O.E.Schulz.[Methods]The contents of Al,As,B,Ba,Ca,Cd,Co,Cr,Cu,Fe,Hg,K,Mg,Mn,Mo,Na,Ni,P,Pb,Pd,Rb,Se,Si,Sn,Sr,Ti,Tl,V and Zn in the C.tangutorum O.E.Schulz samples were determined simultaneously by wet digestion and inductively coupled plasma optical emission spectroscopy(ICP-OES),and the determination results were analyzed by principal component analysis.[Results]A total of 28 inorganic elements were detected in the samples of C.tangutorum O.E.Schulz from different origins.The contents of heavy metals did not exceed the limits.Among the elements,the determined values of Hg in all samples were below the detection limit,so it was not detected.The contents of Ca,Al,P and Fe were relatively high,and they were essential nutrients in the human body.There were significant differences among different elements.Samples from different origins show similar patterns in the distribution of main elements.Correlation analysis shows that 15 pairs of inorganic elements out of 24 elements had extremely significant positive correlations,and 26 pairs of elements had significant positive correlations.The principal component analysis determined Al,As,Ba,Ca,Cd,Fe,Mn,Ni,P,Pb,Pd,Se,V and Zn were the characteristic inorganic elements in the samples of C.tangutorum O.E.Schulz.[Conclusions]The contents of toxic heavy metals in C.tangutorum O.E.Schulz samples from different origins met the edible requirements.The method was simple,rapid and accurate,and could be used to analyze and determine the inorganic elements in the samples of C.tangutorum O.E.Schulz,providing a theoretical basis for the better development and utilization of C.tangutorum O.E.Schulz.展开更多
UBE2O is a distinctive ubiquitin-conjugating enzyme characterized by its large size(1292 residues)and dual E2/E3 enzymatic activities,enabling diverse ubiquitylation types.Unlike typical E2 enzymes(150~200 residues),U...UBE2O is a distinctive ubiquitin-conjugating enzyme characterized by its large size(1292 residues)and dual E2/E3 enzymatic activities,enabling diverse ubiquitylation types.Unlike typical E2 enzymes(150~200 residues),UBE2O’s multifunctionality allows it to regulate substrate degradation,subcellular localization,and functional modulation.Emerging studies highlight its critical roles in protein quality control,erythroid differentiation,metabolic regulation,and maintenance of circadian rhythm.Dysregulation of UBE2O is implicated in various diseases,including cancers,neurodegenerative disorders,and metabolic diseases.This review extensively discusses the unique structural features,diverse biological functions,and pathological roles of UBE2O,as well as its therapeutic potential for associated diseases.展开更多
采用RAPD分子标记技术对湘鄂5个不同区域的堇叶碎米荠(Cardamine violifolia O. E. Schulz)进行居群间遗传多样性分析,从50个随机引物中筛选出10个条带清晰、多态性明显且重复性好的引物,共扩增出78条DNA片段,其中49条为多态性条带,多...采用RAPD分子标记技术对湘鄂5个不同区域的堇叶碎米荠(Cardamine violifolia O. E. Schulz)进行居群间遗传多样性分析,从50个随机引物中筛选出10个条带清晰、多态性明显且重复性好的引物,共扩增出78条DNA片段,其中49条为多态性条带,多态率达62.8%。利用NTSYS 2.10软件进行聚类分析,以相似系数0.68为标准,可将所有居群分为3类,第Ⅰ类为自选品种鄂碎米荠1号和恩施双河鱼塘坝居群;第Ⅱ类为宜昌长阳四方洞居群和湖南常德石门壶瓶山居群;宜昌五峰栗子坪居群独立分为一类。展开更多
基金National Key Research and Development Program of China(2018YFC1708005)Applied Basic Research Plan of Sichuan Province(2018ZY0842)Fundamental Research Funds for the Central Universities(2020NGD01).
文摘[Objectives]This study aimed to optimize the extraction process of total flavonoids in Cardamine tangutorum O.E.Schulz.[Methods]On the basis of single-factor experiments,the ultrasonic-enzymatic extraction process of total flavonoids in C.tangutorum O.E.Schulz was optimized by Box-Behnken design from aspects of ethanol volume fraction,extraction time,extraction temperature and enzyme addition,with yield of total flavonoids as the evaluation index.[Results]The optimal extraction process of total flavonoids in C.tangutorum O.E.Schulz was as follows:ethanol volume fraction of 70%,ultrasonic extraction time of 43 min,ultrasonic extraction temperature of 37℃and cellulose addition of 0.07 mg.Under the optimal conditions,the yield of total flavonoids from C.tangutorum O.E.Schulz reached 75.90 mg/g.[Conclusions]The optimized extraction process was reasonable and feasible,and it could provide a reference for the extraction of total flavonoids in C.tangutorum O.E.Schulz.This study provided a foundation for the quality control of food/medicinal materials and the development and research of big health products of C.tangutorum O.E.Schulz.
基金National Key Research&Development Program of China(2018YFC1708005)Applied Basic Research Program of Sichuan Province(2018ZY0342)Fundamental Research Funds for the Central Universities(2020NGD01).
文摘[Objectives]This study aimed to determine the contents of inorganic elements in Cardamine tangutorum O.E.Schulz samples from different origins to provide a scientific reference for the quality control,safety evaluation and clinical medicinal use of C.tangutorum O.E.Schulz.[Methods]The contents of Al,As,B,Ba,Ca,Cd,Co,Cr,Cu,Fe,Hg,K,Mg,Mn,Mo,Na,Ni,P,Pb,Pd,Rb,Se,Si,Sn,Sr,Ti,Tl,V and Zn in the C.tangutorum O.E.Schulz samples were determined simultaneously by wet digestion and inductively coupled plasma optical emission spectroscopy(ICP-OES),and the determination results were analyzed by principal component analysis.[Results]A total of 28 inorganic elements were detected in the samples of C.tangutorum O.E.Schulz from different origins.The contents of heavy metals did not exceed the limits.Among the elements,the determined values of Hg in all samples were below the detection limit,so it was not detected.The contents of Ca,Al,P and Fe were relatively high,and they were essential nutrients in the human body.There were significant differences among different elements.Samples from different origins show similar patterns in the distribution of main elements.Correlation analysis shows that 15 pairs of inorganic elements out of 24 elements had extremely significant positive correlations,and 26 pairs of elements had significant positive correlations.The principal component analysis determined Al,As,Ba,Ca,Cd,Fe,Mn,Ni,P,Pb,Pd,Se,V and Zn were the characteristic inorganic elements in the samples of C.tangutorum O.E.Schulz.[Conclusions]The contents of toxic heavy metals in C.tangutorum O.E.Schulz samples from different origins met the edible requirements.The method was simple,rapid and accurate,and could be used to analyze and determine the inorganic elements in the samples of C.tangutorum O.E.Schulz,providing a theoretical basis for the better development and utilization of C.tangutorum O.E.Schulz.
基金Supported by Special Projects in Key Areas for Guangdong Provincial Colleges and Universities (No.2021ZDZX2009)Guangzhou Medical University Discipline Construction Funds (Basic Medicine)(No.JCXKJS2022A05)。
文摘UBE2O is a distinctive ubiquitin-conjugating enzyme characterized by its large size(1292 residues)and dual E2/E3 enzymatic activities,enabling diverse ubiquitylation types.Unlike typical E2 enzymes(150~200 residues),UBE2O’s multifunctionality allows it to regulate substrate degradation,subcellular localization,and functional modulation.Emerging studies highlight its critical roles in protein quality control,erythroid differentiation,metabolic regulation,and maintenance of circadian rhythm.Dysregulation of UBE2O is implicated in various diseases,including cancers,neurodegenerative disorders,and metabolic diseases.This review extensively discusses the unique structural features,diverse biological functions,and pathological roles of UBE2O,as well as its therapeutic potential for associated diseases.
文摘采用RAPD分子标记技术对湘鄂5个不同区域的堇叶碎米荠(Cardamine violifolia O. E. Schulz)进行居群间遗传多样性分析,从50个随机引物中筛选出10个条带清晰、多态性明显且重复性好的引物,共扩增出78条DNA片段,其中49条为多态性条带,多态率达62.8%。利用NTSYS 2.10软件进行聚类分析,以相似系数0.68为标准,可将所有居群分为3类,第Ⅰ类为自选品种鄂碎米荠1号和恩施双河鱼塘坝居群;第Ⅱ类为宜昌长阳四方洞居群和湖南常德石门壶瓶山居群;宜昌五峰栗子坪居群独立分为一类。
