Motor reinnervation after repair of tibial nerve defects using autologous vein grafts in rats has previously been reported, but sensory reinnervation after the same repair has not been fully investigated. In this stud...Motor reinnervation after repair of tibial nerve defects using autologous vein grafts in rats has previously been reported, but sensory reinnervation after the same repair has not been fully investigated. In this study, partial sensory reinnervation of muscle spindles was observed after repair of lO-mm left tibial nerve defects using autologous vein grafts with end-to-end anasto- mosis in rats, and functional recovery was confirmed by electrophysiological studies. There were no significant differences in the number, size, or electrophysiological function of reinnervated muscle spindles between the two experimental groups. These findings suggest that repair of short nerve defects with autologous vein grafts provides comparable results to immediate end-to-end anastomosis in terms of sensory reinnervation of muscle spindles.展开更多
Objective To study the morphological changes of soleus muscle spindle and electrical activity of neurons in Red Nucleus(RN) of the rat after 2 weeks' simulated weightlessness, and to reveal the interaction between...Objective To study the morphological changes of soleus muscle spindle and electrical activity of neurons in Red Nucleus(RN) of the rat after 2 weeks' simulated weightlessness, and to reveal the interaction between proprioceptive inputs of muscle spindles and reciprocal alterations in RN under simulated weightlessness. Methods Twenty female rats were exposed to weightlessness simulated by tail-suspension for 14 days (SW-14d). Body weight(200-220g) matched female rats were control group(Con). The morphological changes in isolated muscle spindle of soleus muscle, the discharges of red nucleus neurons were observed after 14d tail-suspensions by silver staining and extracellular recording respectively. Results Compared with control group ,the nerve ending of muscle spindle in SW-14d was distorted, degenerated and dissolved; the diameters of intrafusal fibers and capsule in equatorial region of soleus muscle spindles were diminished(P<0.05). The spontaneous cell activity and discharge of RN neurons (spikes/s) induced by afferent firing from muscle spindles after injection of succinylcholine were reduced after 2 weeks' simulated weightlessness respectively (18.44±5.96 vs. 10.19±6.88, 32.50±8.08 vs. 16.86±5.97, P<0.01). Conclusion The degeneration of muscle spindle induced by simulated weightlessness may be one of the causes that led to alterations in discharges of RN.展开更多
To date, the medium and long-term space flight is urgent in need and has become a major task of our manned space flight program. There is no doubt that medium and long-term space flight has serious damaging impact upo...To date, the medium and long-term space flight is urgent in need and has become a major task of our manned space flight program. There is no doubt that medium and long-term space flight has serious damaging impact upon human physiological systems. For instance, atrophy of the lower limb anti-gravity muscle can be induced during the space flight. Muscle atrophy significantly affects the flight of astronauts in space. Most importantly, it influences the precise manipulation of the astronauts and their response capacity to emergencies on returning to the atmosphere from space. Muscle atrophy caused by weightlessness may also seriously disrupt the normal life and ＇work of the astronauts during the re-adaptation period. Here we summarize the corresponding research concentrating on weightlessness-induced changes of muscular structure and function. By combining research on muscle pain, which is a common clinical pain disease, we further provide a hypothesis concerning a dynamic feedback model of ＂weightlessness condition → muscular atrophy ←→ muscle pain＂. This may be useful to explore the neural mechanisms underlying the occurrence and development of muscular atrophy and muscle pain, through the key study of muscle spindle, and furthermore provide more effective therapy for clinical treatment.展开更多
Objective To analyse the antinociceptive effect of muscle spindle afferents and the involved mechanism.Methods The single unit of wide dynamic range neurons in the spinal cord dorsal horn were recorded extracelluarly....Objective To analyse the antinociceptive effect of muscle spindle afferents and the involved mechanism.Methods The single unit of wide dynamic range neurons in the spinal cord dorsal horn were recorded extracelluarly.The effects of muscle spindle afferents elicited by intravenous administration of succinylcholine on nociceptive responses (C fibres evoked responses,C responses) of WDR neurons were observed before and after bilateral lesions of ventrolateral periaqueduct gray .And the effects of muscle spindle afferents on the spontaneous discharge of the tail flick related cell in the rostral ventro medial medulla and on the spontaneous discharge of the PAG neurons were observed.Results The C responses of WDR neurons were significantly inhibited by muscle spindle afferents,and the inhibitory effects were reduced by bilateral lesions of ventrolateral PAG.The spontaneous discharge of the off cell in the RVM was excited while the on cell was inhibited by intravenous administration of Sch.The spontaneous discharge of the PAG neurons were excited by muscle spindle afferents.Conclusion Muscle spindle afferents show a distinct effect of antinociception.PAG RVM descending inhibitory system may play an important role in this nociceptive modulative mechanism.展开更多
Objective In drugs for i nvigorating blood circulation, to find a herb that can stimulate afferent discha rge of muscle spindle. Methods A single muscle spindle was isolated from sartorial mus cle of toad. Using ai...Objective In drugs for i nvigorating blood circulation, to find a herb that can stimulate afferent discha rge of muscle spindle. Methods A single muscle spindle was isolated from sartorial mus cle of toad. Using air-gap technique, afferent discharge of the muscle spindle was recorded. Effects of Angelica Sinensis, Salvia Miltiorrhiza, and Safflower o n afferent discharge of the muscle spindle were observed. Results Angelica Sinensis could distinctly increase afferent di scharge frequency of the muscle spindle, and this increase was dose-dependent. But Salvia Miltiorrhiza and Safflower had no this excitatory effect. Conclusion It is known that Angelica Sinensis can invigorate bl ood circulation, and we have found its excitatory effect on muscle spindle which makes it possible to serve people with muscle atrophy if more evidences from cl inical experiments are available.展开更多
Objective To analyse the antinociceptive effect of red nucleus (RN) and its role in the antinociceptive effect of muscle spindle afferents. Methods The single units of RN or wide dynamic range (WDR) neuron in the sp...Objective To analyse the antinociceptive effect of red nucleus (RN) and its role in the antinociceptive effect of muscle spindle afferents. Methods The single units of RN or wide dynamic range (WDR) neuron in the spinal cord dorsal horn were extracelluarly recorded. The effects of RN stimulation on nociceptive responses (C fibers evoked responses, C responses) of WDR neurons were observed. The influence of muscle spindle afferents elicited by intravenous administration of succinylcholine (Sch) on the spontaneous discharge of RN neurons and on C responses of WDR neurons were observed. The effect of muscle spindle afferents on C responses of WDR neurons after unilateral lesions of RN was also observed. Results Electrical stimulation of the RN produced a significantly inhibitory effect on the nociceptive responses of WDR neurons. RN neurons were excited by muscle spindle afferents. Muscle spindle afferents significantly inhibited C response of WDR neurons and this inhibitory effect was reduced by lesions of RN. Conclusion RN neurons have a significant antinociceptive effect and might be involved in the antinociceptive effects elicited by muscle spindle afferents.展开更多
Objective To compare the cross section area (CSA) and the immunoreactivity of conjugated ubiquitin in soleus extrafusal and intrafusal fibers after simulated microgravity and to demonstrate the role of muscle spin...Objective To compare the cross section area (CSA) and the immunoreactivity of conjugated ubiquitin in soleus extrafusal and intrafusal fibers after simulated microgravity and to demonstrate the role of muscle spindle in muscle atrophy induced by simulated microgravity. Methods The immunohistochemical technique (ABC) and image analysis were used to assess the conjugated ubiquitin immunostaining and the cross sectional area of intrafusal and extrafusal fibers of soleus in simulated microgravity rats. Results ①Tail suspension caused a progressive loss of soleus mass. Mean fiber CSA of extrafusal fibers were (7±2)%, (21±4)% and (32±7)% smaller after 3 days, 7 days and 14 days suspension, respectively. While the CAS of intrafusal fibers (bag + chain fibers) were (14±3)% ( P < 0.05 ), (30±7)% ( P < 0.01 ) and (44±10)% ( P < 0.01 ) smaller after 3 days, 7 days and 14 days suspension. ② The immunoreactivity of conjugated ubiquitin both in extrafusal and intrafusal fibers increased after tail suspension. The immunoreactivity of intrafusal fibers increased 1 day after suspension and reached the hightest level at 3 days after tail suspension. The immunoreactivity of extrafusal fibers increased after 3 days suspension and reached the highest level after 7 days tail suspension, which was lower than that in intrafusal fibers after 3 days tail suspension. Conclusion These results suggest that soleus atrophy of intrafusal fibers caused by tail suspension is earlier and more severe than that in extrafusal fibers.展开更多
Objective To investigate the role of NRM in the antinociceptive effect of muscle spindle afferents,the influence of NRM lesion on the inhibitory effect of muscle spindle afferents on the nociceptive responses of wide ...Objective To investigate the role of NRM in the antinociceptive effect of muscle spindle afferents,the influence of NRM lesion on the inhibitory effect of muscle spindle afferents on the nociceptive responses of wide dynamic range (WDR) neurons and the effects of the muscle spindle afferents on the NRM neuronal activities were observed. Methods The single units of WDR neurons in the spinal dorsal horn were recorded extracellularly, and the inhibitory effects of activating muscle spindle afferents by intravenous administration of succinylcholine (SCH) on the C fibers evoked responses (C responses) of WDR neurons were tested before and after lesion of NRM.The effects of the muscle spindle afferents activated by administrating SCH on the single NRM neurons were also examined.Results ①It was found that the C responses of WDR neurons were significantly inhibited by intravenously administration of SCH, and the inhibitory effect was reduced after lesion of NRM;②The activities of most of the NRM neurons could be changed significantly by administrating SCH. According to their responses, NRM neurons could be classified into three types:excitatory, inhibitory and non responsive neurons, and the responses were dose dependent. Conclusion These results suggest that the muscle spindle afferents evoked by SCH may activate the NRM neurons, which plays an important role in the antinociception of muscle spindle afferents.展开更多
Background:The perception of effort is a key determinant of endurance performance and a barrier to physical activity in inactive populations.From a neurophysiological viewpoint,effort perception is thought to arise fr...Background:The perception of effort is a key determinant of endurance performance and a barrier to physical activity in inactive populations.From a neurophysiological viewpoint,effort perception is thought to arise from the brain processing of an efference copy of the motor command in sensory areas.However,recent research suggests that feedback from muscle spindles plays a significant role in this perception.In this study,tendon vibration protocols were employed to attenuate sensory feedback during subsequent cycling exercises.The aim was to assess whether vibration would increase cycling power output,muscle activation,and heart rate at fixed perceived effort intensities.Methods:Fifteen healthy young participants completed 2 experimental visits(vibration and sham).In each visit,participants performed two 3-min cycling bouts,1 at a moderate perceived effort intensity and 1 at a strong perceived effort intensity,before(pre)and after(post)an actual or a sham vibration protocol.Vibration was applied bilaterally on the patellar and Achilles tendons for 10 min.Power output,heart rate,and vastus lateralis electromyography(VL EMG)were recorded and averaged for each bout.Absolute values as well as relative change(%)between pre and post conditions were compared across sham and vibration conditions.Results:At moderate perceived effort,power output,heart rate,and VL EMG increased post-vibration compared to pre-vibration(p<0.05),while no difference was observed in the sham condition.At strong perceived effort,power output and VL EMG decreased post-sham(p<0.05)but remained unchanged post-vibration.Moreover,the relative change between pre and post conditions was significantly higher in the vibration conditions compared to the sham condition for all variables.Conclusion:This study shows that tendon vibration reduces effort perception during subsequent cycling bouts.This effect is likely a consequence of vibration-induced reduction in muscle spindle reafferent signaling to the brain,but this mechanism remains to be further elucidated.From an applied perspective,these findings highlight tendon vibration as a promising tool for enhancing physical activity engagement.展开更多
Motoneuron is the control unit of skeletal muscles,and the dynamic frequency-regulating feedback from the afferent nerve of receptors like muscle spindles forms the physical basis of its closed-loop regulation.Focused...Motoneuron is the control unit of skeletal muscles,and the dynamic frequency-regulating feedback from the afferent nerve of receptors like muscle spindles forms the physical basis of its closed-loop regulation.Focused on the synapses of muscle spindle afferents,this paper established a dynamical system-Markov model starting from presynaptic stimulations to postsynaptic responses,and further verified the model via comparisons between theoretical results and relevant experimental data.With the purpose of describing the active features of dendritic membrane,we employed the methods of dynamical systems rather than the traditional passive cable theory,and identified the physical meaning of parameters involved.For the dynamic behavior of postsynaptic currents,we adopted simplified Markov models so that the analytical solutions for the open dynamics of postsynaptic receptors can be obtained.The model in this paper is capable of simulating the actual non-uniformity of channel density,and is suitable for complex finite element analysis of neurons;thus it facilitates the exploration of the frequency-regulating feedback and control mechanisms of motoneurons.展开更多
基金supported by the Medical Science and Technology Innovation Foundation of Nanjing Military Area Command of Chinese PLA in 2013,ZX22
文摘Motor reinnervation after repair of tibial nerve defects using autologous vein grafts in rats has previously been reported, but sensory reinnervation after the same repair has not been fully investigated. In this study, partial sensory reinnervation of muscle spindles was observed after repair of lO-mm left tibial nerve defects using autologous vein grafts with end-to-end anasto- mosis in rats, and functional recovery was confirmed by electrophysiological studies. There were no significant differences in the number, size, or electrophysiological function of reinnervated muscle spindles between the two experimental groups. These findings suggest that repair of short nerve defects with autologous vein grafts provides comparable results to immediate end-to-end anastomosis in terms of sensory reinnervation of muscle spindles.
基金This project was supported by the National Natural Science Foundation of China (No.30300106)
文摘Objective To study the morphological changes of soleus muscle spindle and electrical activity of neurons in Red Nucleus(RN) of the rat after 2 weeks' simulated weightlessness, and to reveal the interaction between proprioceptive inputs of muscle spindles and reciprocal alterations in RN under simulated weightlessness. Methods Twenty female rats were exposed to weightlessness simulated by tail-suspension for 14 days (SW-14d). Body weight(200-220g) matched female rats were control group(Con). The morphological changes in isolated muscle spindle of soleus muscle, the discharges of red nucleus neurons were observed after 14d tail-suspensions by silver staining and extracellular recording respectively. Results Compared with control group ,the nerve ending of muscle spindle in SW-14d was distorted, degenerated and dissolved; the diameters of intrafusal fibers and capsule in equatorial region of soleus muscle spindles were diminished(P<0.05). The spontaneous cell activity and discharge of RN neurons (spikes/s) induced by afferent firing from muscle spindles after injection of succinylcholine were reduced after 2 weeks' simulated weightlessness respectively (18.44±5.96 vs. 10.19±6.88, 32.50±8.08 vs. 16.86±5.97, P<0.01). Conclusion The degeneration of muscle spindle induced by simulated weightlessness may be one of the causes that led to alterations in discharges of RN.
基金supported by the National Natural Science Foundation of China (No. 30971424)
文摘To date, the medium and long-term space flight is urgent in need and has become a major task of our manned space flight program. There is no doubt that medium and long-term space flight has serious damaging impact upon human physiological systems. For instance, atrophy of the lower limb anti-gravity muscle can be induced during the space flight. Muscle atrophy significantly affects the flight of astronauts in space. Most importantly, it influences the precise manipulation of the astronauts and their response capacity to emergencies on returning to the atmosphere from space. Muscle atrophy caused by weightlessness may also seriously disrupt the normal life and ＇work of the astronauts during the re-adaptation period. Here we summarize the corresponding research concentrating on weightlessness-induced changes of muscular structure and function. By combining research on muscle pain, which is a common clinical pain disease, we further provide a hypothesis concerning a dynamic feedback model of ＂weightlessness condition → muscular atrophy ←→ muscle pain＂. This may be useful to explore the neural mechanisms underlying the occurrence and development of muscular atrophy and muscle pain, through the key study of muscle spindle, and furthermore provide more effective therapy for clinical treatment.
文摘Objective To analyse the antinociceptive effect of muscle spindle afferents and the involved mechanism.Methods The single unit of wide dynamic range neurons in the spinal cord dorsal horn were recorded extracelluarly.The effects of muscle spindle afferents elicited by intravenous administration of succinylcholine on nociceptive responses (C fibres evoked responses,C responses) of WDR neurons were observed before and after bilateral lesions of ventrolateral periaqueduct gray .And the effects of muscle spindle afferents on the spontaneous discharge of the tail flick related cell in the rostral ventro medial medulla and on the spontaneous discharge of the PAG neurons were observed.Results The C responses of WDR neurons were significantly inhibited by muscle spindle afferents,and the inhibitory effects were reduced by bilateral lesions of ventrolateral PAG.The spontaneous discharge of the off cell in the RVM was excited while the on cell was inhibited by intravenous administration of Sch.The spontaneous discharge of the PAG neurons were excited by muscle spindle afferents.Conclusion Muscle spindle afferents show a distinct effect of antinociception.PAG RVM descending inhibitory system may play an important role in this nociceptive modulative mechanism.
文摘Objective In drugs for i nvigorating blood circulation, to find a herb that can stimulate afferent discha rge of muscle spindle. Methods A single muscle spindle was isolated from sartorial mus cle of toad. Using air-gap technique, afferent discharge of the muscle spindle was recorded. Effects of Angelica Sinensis, Salvia Miltiorrhiza, and Safflower o n afferent discharge of the muscle spindle were observed. Results Angelica Sinensis could distinctly increase afferent di scharge frequency of the muscle spindle, and this increase was dose-dependent. But Salvia Miltiorrhiza and Safflower had no this excitatory effect. Conclusion It is known that Angelica Sinensis can invigorate bl ood circulation, and we have found its excitatory effect on muscle spindle which makes it possible to serve people with muscle atrophy if more evidences from cl inical experiments are available.
基金ThisresearchwassupportedbytheNationalNaturalScienceFoundationofChina (No .3 90 70 3 3 4)
文摘Objective To analyse the antinociceptive effect of red nucleus (RN) and its role in the antinociceptive effect of muscle spindle afferents. Methods The single units of RN or wide dynamic range (WDR) neuron in the spinal cord dorsal horn were extracelluarly recorded. The effects of RN stimulation on nociceptive responses (C fibers evoked responses, C responses) of WDR neurons were observed. The influence of muscle spindle afferents elicited by intravenous administration of succinylcholine (Sch) on the spontaneous discharge of RN neurons and on C responses of WDR neurons were observed. The effect of muscle spindle afferents on C responses of WDR neurons after unilateral lesions of RN was also observed. Results Electrical stimulation of the RN produced a significantly inhibitory effect on the nociceptive responses of WDR neurons. RN neurons were excited by muscle spindle afferents. Muscle spindle afferents significantly inhibited C response of WDR neurons and this inhibitory effect was reduced by lesions of RN. Conclusion RN neurons have a significant antinociceptive effect and might be involved in the antinociceptive effects elicited by muscle spindle afferents.
文摘Objective To compare the cross section area (CSA) and the immunoreactivity of conjugated ubiquitin in soleus extrafusal and intrafusal fibers after simulated microgravity and to demonstrate the role of muscle spindle in muscle atrophy induced by simulated microgravity. Methods The immunohistochemical technique (ABC) and image analysis were used to assess the conjugated ubiquitin immunostaining and the cross sectional area of intrafusal and extrafusal fibers of soleus in simulated microgravity rats. Results ①Tail suspension caused a progressive loss of soleus mass. Mean fiber CSA of extrafusal fibers were (7±2)%, (21±4)% and (32±7)% smaller after 3 days, 7 days and 14 days suspension, respectively. While the CAS of intrafusal fibers (bag + chain fibers) were (14±3)% ( P < 0.05 ), (30±7)% ( P < 0.01 ) and (44±10)% ( P < 0.01 ) smaller after 3 days, 7 days and 14 days suspension. ② The immunoreactivity of conjugated ubiquitin both in extrafusal and intrafusal fibers increased after tail suspension. The immunoreactivity of intrafusal fibers increased 1 day after suspension and reached the hightest level at 3 days after tail suspension. The immunoreactivity of extrafusal fibers increased after 3 days suspension and reached the highest level after 7 days tail suspension, which was lower than that in intrafusal fibers after 3 days tail suspension. Conclusion These results suggest that soleus atrophy of intrafusal fibers caused by tail suspension is earlier and more severe than that in extrafusal fibers.
基金This research was supported by the National Natural Science Foundation of China(No.390 70 344)
文摘Objective To investigate the role of NRM in the antinociceptive effect of muscle spindle afferents,the influence of NRM lesion on the inhibitory effect of muscle spindle afferents on the nociceptive responses of wide dynamic range (WDR) neurons and the effects of the muscle spindle afferents on the NRM neuronal activities were observed. Methods The single units of WDR neurons in the spinal dorsal horn were recorded extracellularly, and the inhibitory effects of activating muscle spindle afferents by intravenous administration of succinylcholine (SCH) on the C fibers evoked responses (C responses) of WDR neurons were tested before and after lesion of NRM.The effects of the muscle spindle afferents activated by administrating SCH on the single NRM neurons were also examined.Results ①It was found that the C responses of WDR neurons were significantly inhibited by intravenously administration of SCH, and the inhibitory effect was reduced after lesion of NRM;②The activities of most of the NRM neurons could be changed significantly by administrating SCH. According to their responses, NRM neurons could be classified into three types:excitatory, inhibitory and non responsive neurons, and the responses were dose dependent. Conclusion These results suggest that the muscle spindle afferents evoked by SCH may activate the NRM neurons, which plays an important role in the antinociception of muscle spindle afferents.
基金supported by the French National Research Agency(Agence Nationale de la Recherche,ANR,Grant No.ANR-23-CE37-0014)for the Effort_(LESS)Project.
文摘Background:The perception of effort is a key determinant of endurance performance and a barrier to physical activity in inactive populations.From a neurophysiological viewpoint,effort perception is thought to arise from the brain processing of an efference copy of the motor command in sensory areas.However,recent research suggests that feedback from muscle spindles plays a significant role in this perception.In this study,tendon vibration protocols were employed to attenuate sensory feedback during subsequent cycling exercises.The aim was to assess whether vibration would increase cycling power output,muscle activation,and heart rate at fixed perceived effort intensities.Methods:Fifteen healthy young participants completed 2 experimental visits(vibration and sham).In each visit,participants performed two 3-min cycling bouts,1 at a moderate perceived effort intensity and 1 at a strong perceived effort intensity,before(pre)and after(post)an actual or a sham vibration protocol.Vibration was applied bilaterally on the patellar and Achilles tendons for 10 min.Power output,heart rate,and vastus lateralis electromyography(VL EMG)were recorded and averaged for each bout.Absolute values as well as relative change(%)between pre and post conditions were compared across sham and vibration conditions.Results:At moderate perceived effort,power output,heart rate,and VL EMG increased post-vibration compared to pre-vibration(p<0.05),while no difference was observed in the sham condition.At strong perceived effort,power output and VL EMG decreased post-sham(p<0.05)but remained unchanged post-vibration.Moreover,the relative change between pre and post conditions was significantly higher in the vibration conditions compared to the sham condition for all variables.Conclusion:This study shows that tendon vibration reduces effort perception during subsequent cycling bouts.This effect is likely a consequence of vibration-induced reduction in muscle spindle reafferent signaling to the brain,but this mechanism remains to be further elucidated.From an applied perspective,these findings highlight tendon vibration as a promising tool for enhancing physical activity engagement.
基金supported by the National Natural Science Foundation of China (61075101)the National Basic Research Program of China(2011CB013203)the Science and Technology Intercrossing Research Foundation of Shanghai Jiao Tong University (LG2011ZD106)
文摘Motoneuron is the control unit of skeletal muscles,and the dynamic frequency-regulating feedback from the afferent nerve of receptors like muscle spindles forms the physical basis of its closed-loop regulation.Focused on the synapses of muscle spindle afferents,this paper established a dynamical system-Markov model starting from presynaptic stimulations to postsynaptic responses,and further verified the model via comparisons between theoretical results and relevant experimental data.With the purpose of describing the active features of dendritic membrane,we employed the methods of dynamical systems rather than the traditional passive cable theory,and identified the physical meaning of parameters involved.For the dynamic behavior of postsynaptic currents,we adopted simplified Markov models so that the analytical solutions for the open dynamics of postsynaptic receptors can be obtained.The model in this paper is capable of simulating the actual non-uniformity of channel density,and is suitable for complex finite element analysis of neurons;thus it facilitates the exploration of the frequency-regulating feedback and control mechanisms of motoneurons.
