目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期...目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期临床个体化干预提供参考。方法选取2020年3月—2025年3月河北省胸科医院收治的TRALI患者249例为研究对象,根据输血后30 d内预后情况,分别纳入预后良好组(178例)、预后不良组(71例)。比较2组一般临床资料及血清SP-D、MUC1、A20水平,Logistic回归分析血清SP-D、MUC1、A20水平对TRALI患者预后不良的影响因素,受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SP-D、MUC1、A20水平单独及联合检测对预后不良的预测效能,并采用一致性分析进行外部验证。结果2组输血次数、发血至输血时间、过敏史、急性生理与慢性健康评分系统Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分比较,差异有统计学意义(P<0.05);预后不良组血清SP-D、MUC1、A20水平分别为(89.54±21.36)g/L、(22.97±5.14)kU/L、(14.53±1.96)mg/L,明显高于预后良好组的(78.61±18.05)g/L、(16.28±4.32)kU/L、(12.67±1.84)mg/L,差异有统计学意义(P<0.05);Logistic回归分析显示,输血次数、发血至输血时间、过敏史均是TRALI患者预后不良的影响因素(P<0.05),APACHEⅡ评分及血清SP-D、MUC1、A20均与TRALI患者预后不良显著相关(P<0.05);ROC分析显示,血清SP-D、MUC1、A20联合预测预后不良的曲线下面积(area under the curve,AUC)为0.904(95%CI:0.860~0.938),优于各指标单独预测价值(Z=2.507、3.016、3.042,均P<0.05),且外部验证显示,联合预测预后不良与临床实际的符合率为95.00%,Kappa值为0.870(95%CI:0.617~0.982)差异有统计学意义(P<0.05)。结论血清SP-D、MUC1、A20均是TRALI患者预后不良的独立影响因素,联合检测对预后不良具有较高预测价值,可作为TRALI患者预后的潜在预测因子,并可指导临床工作。展开更多
Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,red...Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency.展开更多
文摘目的探讨血清表面活性蛋白D(surfactant protein D,SP-D)、黏蛋白1(mucin 1,MUC1)、锌指蛋白A20(zinc finger protein A20,A20)水平对输血相关性急性肺损伤(transfusion-related acute lung injury,TRALI)患者临床预后的预测价值,以期临床个体化干预提供参考。方法选取2020年3月—2025年3月河北省胸科医院收治的TRALI患者249例为研究对象,根据输血后30 d内预后情况,分别纳入预后良好组(178例)、预后不良组(71例)。比较2组一般临床资料及血清SP-D、MUC1、A20水平,Logistic回归分析血清SP-D、MUC1、A20水平对TRALI患者预后不良的影响因素,受试者工作特征(receiver operating characteristic,ROC)曲线分析血清SP-D、MUC1、A20水平单独及联合检测对预后不良的预测效能,并采用一致性分析进行外部验证。结果2组输血次数、发血至输血时间、过敏史、急性生理与慢性健康评分系统Ⅱ(acute physiology and chronic health evaluationⅡ,APACHEⅡ)评分比较,差异有统计学意义(P<0.05);预后不良组血清SP-D、MUC1、A20水平分别为(89.54±21.36)g/L、(22.97±5.14)kU/L、(14.53±1.96)mg/L,明显高于预后良好组的(78.61±18.05)g/L、(16.28±4.32)kU/L、(12.67±1.84)mg/L,差异有统计学意义(P<0.05);Logistic回归分析显示,输血次数、发血至输血时间、过敏史均是TRALI患者预后不良的影响因素(P<0.05),APACHEⅡ评分及血清SP-D、MUC1、A20均与TRALI患者预后不良显著相关(P<0.05);ROC分析显示,血清SP-D、MUC1、A20联合预测预后不良的曲线下面积(area under the curve,AUC)为0.904(95%CI:0.860~0.938),优于各指标单独预测价值(Z=2.507、3.016、3.042,均P<0.05),且外部验证显示,联合预测预后不良与临床实际的符合率为95.00%,Kappa值为0.870(95%CI:0.617~0.982)差异有统计学意义(P<0.05)。结论血清SP-D、MUC1、A20均是TRALI患者预后不良的独立影响因素,联合检测对预后不良具有较高预测价值,可作为TRALI患者预后的潜在预测因子,并可指导临床工作。
文摘Pretreatment of Low-Density Polyethylene(LDPE)with physicochemical methods before biodegradation has been demonstrated as an effective strategy.The pretreatment of LDPE exhibited alterations in molecular structure,reducing hydrophobicity and decreasing tensile strength.Additionally,pretreating LDPE enhanced microbial biodegradability to improve biofilm formation and significantly reduced the physical weight of LDPE film.AS3–8 consortia exhibited a maximum weight loss of 8.0%±0.5%after 45 days of incubation.While Bacillus sp.AS3 and Sphingobacterium sp.AS8 demonstrated LDPE weight loss of 5.03%±1.6%and 1.6%±0.5%,respectively.The structure of LDPE was altered after incubation with the bacterial strains,resulting in a reduction in the intensity of functional groups,including C=O,C=C,N–H,and C–N.The carbonyl index(CI)of LDPE also decreased by 7.17%after the consortia AS3–8 degradation.Consortia AS3–8 significantly impacted the physical properties of LDPE by reducing the water contact angle(WCA),decreasing to 64.21°±3.69°,and tensile strength(TS),decreasing to 17.97±0.3 MPa.Moreover,the esterase activity was measured through 45 days of incubation.SDS-PAGE analysis of the AS3–8 consortia revealed bands at 35,48,and 70 kDa molecular weights,similar to known enzymes like laccase and esterase.Furthermore,SEM observations showed rough,cracked surfaces on pretreated LDPE,with biofilms present after incubation with the bacterial strains.GC–MS analysis revealed that AS3–8 consortia produced depolymerized chemicals,including alkanes,aldehydes,and esters.The LDPE biodegradation pathway was elucidated.This study addresses critical knowledge gaps in improving plastic degradation efficiency.
