目的 报道1例有输血史和妊娠史的产生抗-Jr^(a)的孕妇的抗体鉴定、妊娠期间血液管理与胎儿新生儿溶血病(hemolytic disease of fetus and newborn,HDFN)的监测过程。方法 利用盐水凝集技术和抗人球蛋白技术完成孕妇血型鉴定、意外抗体...目的 报道1例有输血史和妊娠史的产生抗-Jr^(a)的孕妇的抗体鉴定、妊娠期间血液管理与胎儿新生儿溶血病(hemolytic disease of fetus and newborn,HDFN)的监测过程。方法 利用盐水凝集技术和抗人球蛋白技术完成孕妇血型鉴定、意外抗体筛查与鉴定、抗体效价及IgG亚类检测;利用PCR-SSP技术完成孕妇18个血型系统基因分型,利用二代测序技术完成38个血型系统基因测序,利用一代测序技术验证二代测序检出的稀有血型突变位点并对家系成员完成对应稀有血型基因测序调查。通过孕期门诊贫血管理和自体血采集为孕妇完成备血。对新生儿完成HDFN三项试验及新生儿血浆IgG亚类检测。结果 孕妇血型为B型RhD阳性,意外抗体筛查阳性,抗体鉴定符合高频抗原抗体格局。基因测序发现孕妇为ABCG2 c.376C>T纯合突变所致Jr(a-)表型,血浆中存在抗-Jr^(a)。家系成员中未找到与孕妇相合的Jr(a-)血液。孕妇孕期IgG抗体效价基本维持256,未检测到针对Jr^(a)抗原的IgG1和IgG3亚类抗体。孕期两次储备自体血液800 mL。新生儿为B型RhD阳性,Jr(a+),意外抗体筛查阳性(抗-Jr^(a)),IgG抗体亚类分型未检出IgG1和IgG3,直接抗人球蛋白试验阳性,酸放散阴性。结论 血清学和血型基因分析技术结合可为高频抗原抗体鉴定提供诊断依据,围产期贫血管理和阶段性储备自体血的方式保障患者围术期用血,IgG抗体亚类分型可为临床评估及预防HDFN提供参考依据。展开更多
Jerry W.Ward,Jr.(1943-2025)is a renowned African American scholar and poet.This article presents a critique of Dr.Ward by remembering him as a literary scholar and critic,educator,mentor,and cultural and academic amba...Jerry W.Ward,Jr.(1943-2025)is a renowned African American scholar and poet.This article presents a critique of Dr.Ward by remembering him as a literary scholar and critic,educator,mentor,and cultural and academic ambassador to China and the world.It reveals the uniqueness in Dr.Ward’s critical thinking about African American culture,tradition and history,his importance as an internationally known scholar,his originality in his poetic art,and the value of his legacy for African American literature and its scholarship.展开更多
To construct the eukaryot ic expression system of E1 glycopr otein of rubella virus(RV)JR23 str ain and to study the biological an d immunological properties of the expressed product,the E1 gene was amplified by RT-PC...To construct the eukaryot ic expression system of E1 glycopr otein of rubella virus(RV)JR23 str ain and to study the biological an d immunological properties of the expressed product,the E1 gene was amplified by RT-PCR,sequenced and recombinated with the pBluescript ⅡSK+ vectorThe recombinated vecto r was co-transfected into BHK21 ce ll with vaccinia virus which carri es the gene of T7 RNA polymeraseT he expressed protein was characteri zed with hemadsorption,immunohisto chemistry and indirect immunofluor escence stainingThe results showe d that the recombinated vector,pBS K+-RV E1,was proved containing E1 gene by enzyme digestion and seque ncingThe expressed protein could be detected both in cytoplasm amd on membrane of the transfected cel ls by hemadsorption,immunohistoche mistry and indirect immunofluoresc enceThe E1 protein mainly focused in cytoplasm near the nucleusThes e data proved that the expression vector,pBSK+-RV E1,was successfull y constructed and the glycoprotein E1 of RV JR23 strain was effective ly expressed in BHK21 cell culture This study provides foundations f or studies on the relationships be tween structure and function of RV gene,between structure and functio n of RV proteins,and on the subuni t vaccine of rubella展开更多
文摘Jerry W.Ward,Jr.(1943-2025)is a renowned African American scholar and poet.This article presents a critique of Dr.Ward by remembering him as a literary scholar and critic,educator,mentor,and cultural and academic ambassador to China and the world.It reveals the uniqueness in Dr.Ward’s critical thinking about African American culture,tradition and history,his importance as an internationally known scholar,his originality in his poetic art,and the value of his legacy for African American literature and its scholarship.
文摘To construct the eukaryot ic expression system of E1 glycopr otein of rubella virus(RV)JR23 str ain and to study the biological an d immunological properties of the expressed product,the E1 gene was amplified by RT-PCR,sequenced and recombinated with the pBluescript ⅡSK+ vectorThe recombinated vecto r was co-transfected into BHK21 ce ll with vaccinia virus which carri es the gene of T7 RNA polymeraseT he expressed protein was characteri zed with hemadsorption,immunohisto chemistry and indirect immunofluor escence stainingThe results showe d that the recombinated vector,pBS K+-RV E1,was proved containing E1 gene by enzyme digestion and seque ncingThe expressed protein could be detected both in cytoplasm amd on membrane of the transfected cel ls by hemadsorption,immunohistoche mistry and indirect immunofluoresc enceThe E1 protein mainly focused in cytoplasm near the nucleusThes e data proved that the expression vector,pBSK+-RV E1,was successfull y constructed and the glycoprotein E1 of RV JR23 strain was effective ly expressed in BHK21 cell culture This study provides foundations f or studies on the relationships be tween structure and function of RV gene,between structure and functio n of RV proteins,and on the subuni t vaccine of rubella
