Previous studies have indicated that the Ilex genus exhibits antioxidant, neuroprotective, hepatoprotective, and antiinflammatory activities. However, the pharmacologic action and mechanisms of Ilex cornuta against ca...Previous studies have indicated that the Ilex genus exhibits antioxidant, neuroprotective, hepatoprotective, and antiinflammatory activities. However, the pharmacologic action and mechanisms of Ilex cornuta against cardiac diseases have not yet been explored. The present study was designed to investigate the antioxidant and cardioprotective effects of Ilex cornuta root with in vitro and in vivo models. The anti-oxidative effects of the extract of Ilex cornuta root(ICR) were measured by 2, 2-diphenyl-1- picrylhydrazyl(DPPH) free-radical scavenging and MTT assays as well as immunoassay. Furthermore, a rat model of myocardial ischemia was established to investigate the cardioprotective effect of ICR in vivo. Eight compounds were isolated and identified from ICR and exhibited DPPH free-radical scavenging activities. They also could increase cell viability and inhibit morphological changes induced by H_2O_2 or Na_2S_2O_4 in H9c2 cardiomyocytes, followed by increasing the SOD activities and decreasing the MDA and ROS levels. In addition, it could suppress the apoptosis of cardiomyocytes. In the rat model of myocardial ischemia, ICR decreased myocardial infarct size and suppressed the activities of LDH and CK. Furthermore, ICR attenuated histopathological alterations of heart tissues and the MDA levels, while increasing SOD activities in serum. In conclusion, these results suggest that ICR has cardioprotective activity and could be developed as a new food supplement for the prevention of ischemic heart disease.展开更多
In the present study, three new triterpenoids, 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-α-L-arabinopyranoside(1), 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester(2), and urs-1...In the present study, three new triterpenoids, 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-α-L-arabinopyranoside(1), 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester(2), and urs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester(3), and a known triterpenoid, 3β-hydroxy-urs-2, 18-dien-28-oic acid(4, randialic acid B), were isolated from the aerial parts of Ilex cornuta. Their structures were identified by the spectroscopic analyses(IR, ESI-MS, HR-ESI-MS, and 1D and 2D NMR) and chemical reactions. Compound 4 showed significant cell-protective effects against H_2O_2-induced H9c2 cardiomyocyte injury. Compounds 1-4 did not show any significant DPPH radical scavenging activity.展开更多
The population size class structure, survival curve, height class structure and population distribution patterns of Ilex cornuta in Longgan Lake National Nature Preserve, Hubei Province, were investigated by using the...The population size class structure, survival curve, height class structure and population distribution patterns of Ilex cornuta in Longgan Lake National Nature Preserve, Hubei Province, were investigated by using the adjacent grid method. The result showed that the population age structure of I. cornuta was of middle-aging type, in the vertical space of population individuals, most of them lived in the shrub layer, and a small number of individuals entered the arborous layer. The distribution pattern of I. cornuta was analyzed by variance /mean ratio method, and the results showed that the distribution pattern of the population was the cluster type. This study can provide references for the further protection, research and development and utilization of I. cornuta .展开更多
A new triterpene glycoside, pomolic acid 3-O-6″-methyl-β-D-glucuronopyranosyl-（1→3）-α-L-arabino- pyranoside, and a new flavonol glycoside, quercetin 3-O-β-D-glucopyranosyl-（1→2）-α-L-arabinopyranoside togeth...A new triterpene glycoside, pomolic acid 3-O-6″-methyl-β-D-glucuronopyranosyl-（1→3）-α-L-arabino- pyranoside, and a new flavonol glycoside, quercetin 3-O-β-D-glucopyranosyl-（1→2）-α-L-arabinopyranoside together with three known triterpene saponins and two flavonol glycosides, were isolated from the leaves of Ilex cornuta. Their structures were established on the basis of spectroscopic analysis.展开更多
从枸骨(IlexcornutaLindl.exPaxt.)中分离得到20个化合物,鉴定了16个,分别为:枸骨甙1(Gouguside1,Ⅴ)即坡摸酸3 β O α L 吡喃阿拉伯糖甙;枸骨甙2(Gouguside2,Ⅵ)即3 β O D 吡喃葡萄糖基坡摸酸 β 28 O D 吡喃葡萄糖酯;枸骨甙3(Gougus...从枸骨(IlexcornutaLindl.exPaxt.)中分离得到20个化合物,鉴定了16个,分别为:枸骨甙1(Gouguside1,Ⅴ)即坡摸酸3 β O α L 吡喃阿拉伯糖甙;枸骨甙2(Gouguside2,Ⅵ)即3 β O D 吡喃葡萄糖基坡摸酸 β 28 O D 吡喃葡萄糖酯;枸骨甙3(Gouguside3,Ⅶ)即3 β O (β D 吡喃葡萄糖基) α L 吡喃葡萄糖基坡摸酸 β 28 O D 吡喃葡萄糖酯的类似物;枸骨甙4(Gouguside4,ⅩⅦ)即3 β O (β D 吡喃葡萄糖基) α L 吡喃葡萄糖基坡摸酸 β 28 O D 吡喃葡萄糖酯;枸骨甙5(Gouguside5,ⅩⅧ)即坡摸酸3 β O α L 2′ 乙酰氧基吡喃阿拉伯糖基 28 O β D 吡喃葡萄糖酯,枸骨甙6(Gouguside6,ⅩⅨ)即3 β O (β D 吡喃葡萄糖基) α L 4 乙酰氧基吡喃阿拉伯糖基坡摸酸 β 28 O D 吡喃葡萄糖酯;枸骨甙7(Gouguside7,ⅩⅩ)即3 β O α L 吡喃阿拉伯糖基 28 O β D 吡喃葡萄糖酯;胡萝卜甙(daucostorolⅧ);2,4 二羟基苯甲酸(2,4 dihydroxybenzoicacidⅠ);3,4 二羟基桂皮酸(3,4 dihyroxycinnamunicacidⅡ、Ⅳ);长链脂肪酸或醇5个(Longchainfattyacid,Ⅲ、Ⅵ、Ⅶ、Ⅷ、ⅩⅣ)。在鉴定的16个化合物中,枸骨甙1、枸骨甙2、枸骨甙6和枸骨甙7为首次从枸骨中分离得到,枸骨甙3和枸骨甙4为新化合物。展开更多
采用涂布平板法分离药用植物枸骨可培养内生细菌,采用对峙法和发酵滤液培养法筛选对植物病原菌具高抑制作用的菌株,通过镜检和显微摄影观察处理组菌丝的变化,对16S r DNA PCR产物进行测序和同源性分析菌株的系统发育关系,通过形态、生...采用涂布平板法分离药用植物枸骨可培养内生细菌,采用对峙法和发酵滤液培养法筛选对植物病原菌具高抑制作用的菌株,通过镜检和显微摄影观察处理组菌丝的变化,对16S r DNA PCR产物进行测序和同源性分析菌株的系统发育关系,通过形态、生理生化特征和16S r DNA序列比对确定菌株的分类地位.从枸骨健康植株的根、茎、叶及果实中分离得到85株内生细菌,筛选出对烟草赤星病菌、稻瘟病菌、棉花枯萎病菌等有较强抑菌作用的10株菌,初步鉴定为4属7种.其中,抑菌效果最强的GG78(60.3%)、GG31(48.1%)、GG3(61.0%)分别属于阴沟肠杆菌、路德维希肠杆菌和蜡样芽孢杆菌.受抑制病原菌菌丝均发生畸形、扭曲,局部膨大形成原生质浓缩球、菌丝基部出现毛发状细分支等现象,可能与内生菌分泌到胞外具有一定抑菌或杀菌作用的代谢物质如抗生素、水解酶类、生物碱等有关.展开更多
从枸骨中分离了20个化合物,对其中的3个进行了心血管方面的药理作用研究。结果表明,3,4 二羟基桂皮酸和胡萝卜甙对小鼠缺氧、氯仿诱发的小鼠心室纤颤及脑垂体后叶素诱发的大鼠心肌缺血均无保护作用,也不能改变豚鼠离体心肌的心率、冠脉...从枸骨中分离了20个化合物,对其中的3个进行了心血管方面的药理作用研究。结果表明,3,4 二羟基桂皮酸和胡萝卜甙对小鼠缺氧、氯仿诱发的小鼠心室纤颤及脑垂体后叶素诱发的大鼠心肌缺血均无保护作用,也不能改变豚鼠离体心肌的心率、冠脉流量及心肌收缩力。3 β O (β D 吡喃葡萄糖基) α L 吡喃阿拉伯糖基坡摸酸 β 28 O D 吡喃葡萄糖酯(命名为枸骨甙4)对小鼠缺氧、氯仿诱发的小鼠心室纤颤无保护作用,对脑垂体后叶素诱发的心肌缺血有一定的保护作用,不改变豚鼠离体心肌的心率和冠脉流量,但可显著降低心肌收缩力。展开更多
文摘Previous studies have indicated that the Ilex genus exhibits antioxidant, neuroprotective, hepatoprotective, and antiinflammatory activities. However, the pharmacologic action and mechanisms of Ilex cornuta against cardiac diseases have not yet been explored. The present study was designed to investigate the antioxidant and cardioprotective effects of Ilex cornuta root with in vitro and in vivo models. The anti-oxidative effects of the extract of Ilex cornuta root(ICR) were measured by 2, 2-diphenyl-1- picrylhydrazyl(DPPH) free-radical scavenging and MTT assays as well as immunoassay. Furthermore, a rat model of myocardial ischemia was established to investigate the cardioprotective effect of ICR in vivo. Eight compounds were isolated and identified from ICR and exhibited DPPH free-radical scavenging activities. They also could increase cell viability and inhibit morphological changes induced by H_2O_2 or Na_2S_2O_4 in H9c2 cardiomyocytes, followed by increasing the SOD activities and decreasing the MDA and ROS levels. In addition, it could suppress the apoptosis of cardiomyocytes. In the rat model of myocardial ischemia, ICR decreased myocardial infarct size and suppressed the activities of LDH and CK. Furthermore, ICR attenuated histopathological alterations of heart tissues and the MDA levels, while increasing SOD activities in serum. In conclusion, these results suggest that ICR has cardioprotective activity and could be developed as a new food supplement for the prevention of ischemic heart disease.
文摘In the present study, three new triterpenoids, 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-α-L-arabinopyranoside(1), 23-hydroxyurs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester(2), and urs-12, 18-dien-28-oic acid 3β-O-β-D-glucuronopyranoside-6-O-methyl ester(3), and a known triterpenoid, 3β-hydroxy-urs-2, 18-dien-28-oic acid(4, randialic acid B), were isolated from the aerial parts of Ilex cornuta. Their structures were identified by the spectroscopic analyses(IR, ESI-MS, HR-ESI-MS, and 1D and 2D NMR) and chemical reactions. Compound 4 showed significant cell-protective effects against H_2O_2-induced H9c2 cardiomyocyte injury. Compounds 1-4 did not show any significant DPPH radical scavenging activity.
基金Supported by the Team Project of Hubei Collaborative Innovation Center for the Characteristic Resources Exploitation of Dabie Mountains(2015TD06)
文摘The population size class structure, survival curve, height class structure and population distribution patterns of Ilex cornuta in Longgan Lake National Nature Preserve, Hubei Province, were investigated by using the adjacent grid method. The result showed that the population age structure of I. cornuta was of middle-aging type, in the vertical space of population individuals, most of them lived in the shrub layer, and a small number of individuals entered the arborous layer. The distribution pattern of I. cornuta was analyzed by variance /mean ratio method, and the results showed that the distribution pattern of the population was the cluster type. This study can provide references for the further protection, research and development and utilization of I. cornuta .
文摘A new triterpene glycoside, pomolic acid 3-O-6″-methyl-β-D-glucuronopyranosyl-（1→3）-α-L-arabino- pyranoside, and a new flavonol glycoside, quercetin 3-O-β-D-glucopyranosyl-（1→2）-α-L-arabinopyranoside together with three known triterpene saponins and two flavonol glycosides, were isolated from the leaves of Ilex cornuta. Their structures were established on the basis of spectroscopic analysis.
文摘从枸骨(IlexcornutaLindl.exPaxt.)中分离得到20个化合物,鉴定了16个,分别为:枸骨甙1(Gouguside1,Ⅴ)即坡摸酸3 β O α L 吡喃阿拉伯糖甙;枸骨甙2(Gouguside2,Ⅵ)即3 β O D 吡喃葡萄糖基坡摸酸 β 28 O D 吡喃葡萄糖酯;枸骨甙3(Gouguside3,Ⅶ)即3 β O (β D 吡喃葡萄糖基) α L 吡喃葡萄糖基坡摸酸 β 28 O D 吡喃葡萄糖酯的类似物;枸骨甙4(Gouguside4,ⅩⅦ)即3 β O (β D 吡喃葡萄糖基) α L 吡喃葡萄糖基坡摸酸 β 28 O D 吡喃葡萄糖酯;枸骨甙5(Gouguside5,ⅩⅧ)即坡摸酸3 β O α L 2′ 乙酰氧基吡喃阿拉伯糖基 28 O β D 吡喃葡萄糖酯,枸骨甙6(Gouguside6,ⅩⅨ)即3 β O (β D 吡喃葡萄糖基) α L 4 乙酰氧基吡喃阿拉伯糖基坡摸酸 β 28 O D 吡喃葡萄糖酯;枸骨甙7(Gouguside7,ⅩⅩ)即3 β O α L 吡喃阿拉伯糖基 28 O β D 吡喃葡萄糖酯;胡萝卜甙(daucostorolⅧ);2,4 二羟基苯甲酸(2,4 dihydroxybenzoicacidⅠ);3,4 二羟基桂皮酸(3,4 dihyroxycinnamunicacidⅡ、Ⅳ);长链脂肪酸或醇5个(Longchainfattyacid,Ⅲ、Ⅵ、Ⅶ、Ⅷ、ⅩⅣ)。在鉴定的16个化合物中,枸骨甙1、枸骨甙2、枸骨甙6和枸骨甙7为首次从枸骨中分离得到,枸骨甙3和枸骨甙4为新化合物。
文摘采用涂布平板法分离药用植物枸骨可培养内生细菌,采用对峙法和发酵滤液培养法筛选对植物病原菌具高抑制作用的菌株,通过镜检和显微摄影观察处理组菌丝的变化,对16S r DNA PCR产物进行测序和同源性分析菌株的系统发育关系,通过形态、生理生化特征和16S r DNA序列比对确定菌株的分类地位.从枸骨健康植株的根、茎、叶及果实中分离得到85株内生细菌,筛选出对烟草赤星病菌、稻瘟病菌、棉花枯萎病菌等有较强抑菌作用的10株菌,初步鉴定为4属7种.其中,抑菌效果最强的GG78(60.3%)、GG31(48.1%)、GG3(61.0%)分别属于阴沟肠杆菌、路德维希肠杆菌和蜡样芽孢杆菌.受抑制病原菌菌丝均发生畸形、扭曲,局部膨大形成原生质浓缩球、菌丝基部出现毛发状细分支等现象,可能与内生菌分泌到胞外具有一定抑菌或杀菌作用的代谢物质如抗生素、水解酶类、生物碱等有关.
文摘从枸骨中分离了20个化合物,对其中的3个进行了心血管方面的药理作用研究。结果表明,3,4 二羟基桂皮酸和胡萝卜甙对小鼠缺氧、氯仿诱发的小鼠心室纤颤及脑垂体后叶素诱发的大鼠心肌缺血均无保护作用,也不能改变豚鼠离体心肌的心率、冠脉流量及心肌收缩力。3 β O (β D 吡喃葡萄糖基) α L 吡喃阿拉伯糖基坡摸酸 β 28 O D 吡喃葡萄糖酯(命名为枸骨甙4)对小鼠缺氧、氯仿诱发的小鼠心室纤颤无保护作用,对脑垂体后叶素诱发的心肌缺血有一定的保护作用,不改变豚鼠离体心肌的心率和冠脉流量,但可显著降低心肌收缩力。
