Hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress.Two Hedera helix cultivars,heat-tole...Hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress.Two Hedera helix cultivars,heat-tolerant(HT)‘Jessica’and heat-sensitive(HS)‘Shamrock’,were used for differences analyses of transcriptome.We detected 6179 differentially expressed genes(DEGs)and 5992 DEGs in‘Jessica’and‘Shamrock’to heat stress,respectively.Among these,1983 upregulated DEGs and 1400 downregulated DEGs were shared between both varieties,resulting in enhancement of various pathways such as biosynthesis of secondary metabolites,glyoxylate dicarboxylate metabolism,and protein processing in endoplasmic reticulum(PPER),RNA transport,respectively.Among the common downregulated DEGs,72 TFs in 25 gene families were found,including members of the MYB and MYBrelated families,the bHLH family,etc.In‘Jessica’(HT),634 unique up-regulated DEGs were identified,including genes associated with phenylpropanoid biosynthesis,starch and sucrose metabolism,biosynthesis of amino acids.Most upregulated TFs of HT were upregulated much more rapidly than those of HS in response to high temperature.Eleven TF-encoding genes were selected to verify the RNA sequencing data by qPCR.This study revealed the gene expression patterns of ivy in response to heat stress and the molecular basis of heat tolerance,which provided theoretical references for improving the heat tolerance of ivy.展开更多
Objective: To explore the antihypertensive effect of extracts from the leaves of Hedera helix(H. helix) on normotensive and hypertensive rats in-vivo followed by vasodilatory studies in-vitro.Methods: The crude methan...Objective: To explore the antihypertensive effect of extracts from the leaves of Hedera helix(H. helix) on normotensive and hypertensive rats in-vivo followed by vasodilatory studies in-vitro.Methods: The crude methanolic extract was prepared and the activity directed fractionation was carried out. Spectrophotometric analysis of total phenolic and flavonoid content was also done. HPLC analysis was performed for the detection of hederacoside C. In-vivo blood pressure study was carried out in normotensive and high salt-induced hypertensive SpragueDawley rats. Isolated aortic tissues from rat and rabbit were used for in-vitro studies. The effects were recorded and analyzed through PowerL ab data acquisition system. Results: Crude extract of H. helix(1-30 mg/kg) decreased blood pressure to greater extent in high salt-induced hypertensive rats in-vivo compared to the normotensive [Max. fall(58.59±0.02) mm Hg vs.(67.53±3.07) mmH g]. The n-hexane, chloroform, ethyl acetate and aqueous fractions were also checked. These fractions were more effective in hypertensive rats. Aqueous fraction was more potent and n-hexane the least. In isolated rat aortic rings precontracted with phenylephrine, crude extract induced endothelium-dependent effect. The endothelium-dependent component of vasodilatory effect was ablated with L-NAME, and denudation of endothelium. The aqueous fraction was most potent vasodilator. In aortic rings from hypertensive rats, extract and fractions produced partial endothelium-independent effect which was not affected by pretreatment with L-NAME, indicating endothelium dysfunction in the hypertensive rats and suggesting additional vasodilatory mechanisms. In rabbit aorta, the extract and fractions also inhibited phenylephrine and high K^+-induced precontractions, and shifted Ca^(++) concentration–response curves. Conclusions: Our findings indicate that extract and fractions of H. helix are antihypertensive remedies, which is the outcome of vasodilatory effect. This vasodilatory effect is mediated through nitric oxide and Ca^(++) antagonism.展开更多
Potted seedlings of Hedera nepalensis and H. helix were exposed to formaldehyde and low light alone or in combination for four months, before the morphological and nutritive indices of the plants were measured to asse...Potted seedlings of Hedera nepalensis and H. helix were exposed to formaldehyde and low light alone or in combination for four months, before the morphological and nutritive indices of the plants were measured to assess the effects of formaldehyde and low-light stress on the growth and nutrient accumulation of Hedera species. The results showed that treatment with either formaldehyde or low-light stress reduced leaf length, leaf width, stem length, stem diameter, root length and biomass, but increased leaf shape index of the two Hedera species. Treatment with formaldehyde alone increased root-shoot ratio, while treatment with formaldehyde ＋ low light or low light alone decreased root-shoot ratio of the two Hedera species. Under the stress of formaldehyde, soluble sugar content decreased, while starch content increased in roots, stems and leaves of the two Hedera species; total carbon content, proline content and carbon-to-nitrogen （C/N） ratio increased, while protein content and phosphorus content decreased in roots of the two Hedera species; the contents of total carbon, protein and total nitrogen in leaves of the two Hedera species; C/N ratio in roots of H. nepalensis increased; the C/N ratio in roots of H. nepalensis and that in leaves of the two Hedera species significantly increased. Under formaldehyde ＋ low light stress, the soluble sugar content decreased, while starch content increased in stems and leaves of the two Hedera species; starch content in roots of the two Hedera species decreased; the contents of protein and total nitrogen decreased, while C/N ratio increased in leaves of H. nepalensis ; proline content in roots and stems of the two Hedera species significantly increased.展开更多
[Objectives]The purpose was to establish an induction system for friable callus of Hedera nepalensis var.sinensis with different parts.[Methods]By screening the most suitable explant and adjusting the hormone ratio of...[Objectives]The purpose was to establish an induction system for friable callus of Hedera nepalensis var.sinensis with different parts.[Methods]By screening the most suitable explant and adjusting the hormone ratio of medium,friable calli of H.nepalensis var.sinensis were induced.[Results]The calli could be induced from leaves,petioles and stem segments,but the ideal explant was stem segments,with induction rate reaching 98%.The optimal medium for callus proliferation was MS+0.5 mg/L KT+1.0 mg/L 2,4-D+30.0 g/L sucrose.After 3-4 generations of subculture on MS+0.5 mg/L BA+1.0 mg/L 2,4-D+30.0 g/L sucrose,favorable friable calli of H.nepalensis var.sinensis were obtained.[Conclusions]The friable calli induced in this experiment can lay a foundation for in-vitro regeneration and cellular secondary metabolite production of H.nepalensis var.sinensis.展开更多
基金supported by the Shanghai Agriculture Applied Technology Development Program,China(Grant No.C201702-1)Engineering Research Center Project from Science and Technology Commission of Shanghai Municipality(No.18DZ2283500).
文摘Hedera helix is an evergreen ornamental plant that is resistant to cool but not high temperature and deserves to be further researched for improving its adaptability to heat stress.Two Hedera helix cultivars,heat-tolerant(HT)‘Jessica’and heat-sensitive(HS)‘Shamrock’,were used for differences analyses of transcriptome.We detected 6179 differentially expressed genes(DEGs)and 5992 DEGs in‘Jessica’and‘Shamrock’to heat stress,respectively.Among these,1983 upregulated DEGs and 1400 downregulated DEGs were shared between both varieties,resulting in enhancement of various pathways such as biosynthesis of secondary metabolites,glyoxylate dicarboxylate metabolism,and protein processing in endoplasmic reticulum(PPER),RNA transport,respectively.Among the common downregulated DEGs,72 TFs in 25 gene families were found,including members of the MYB and MYBrelated families,the bHLH family,etc.In‘Jessica’(HT),634 unique up-regulated DEGs were identified,including genes associated with phenylpropanoid biosynthesis,starch and sucrose metabolism,biosynthesis of amino acids.Most upregulated TFs of HT were upregulated much more rapidly than those of HS in response to high temperature.Eleven TF-encoding genes were selected to verify the RNA sequencing data by qPCR.This study revealed the gene expression patterns of ivy in response to heat stress and the molecular basis of heat tolerance,which provided theoretical references for improving the heat tolerance of ivy.
文摘Objective: To explore the antihypertensive effect of extracts from the leaves of Hedera helix(H. helix) on normotensive and hypertensive rats in-vivo followed by vasodilatory studies in-vitro.Methods: The crude methanolic extract was prepared and the activity directed fractionation was carried out. Spectrophotometric analysis of total phenolic and flavonoid content was also done. HPLC analysis was performed for the detection of hederacoside C. In-vivo blood pressure study was carried out in normotensive and high salt-induced hypertensive SpragueDawley rats. Isolated aortic tissues from rat and rabbit were used for in-vitro studies. The effects were recorded and analyzed through PowerL ab data acquisition system. Results: Crude extract of H. helix(1-30 mg/kg) decreased blood pressure to greater extent in high salt-induced hypertensive rats in-vivo compared to the normotensive [Max. fall(58.59±0.02) mm Hg vs.(67.53±3.07) mmH g]. The n-hexane, chloroform, ethyl acetate and aqueous fractions were also checked. These fractions were more effective in hypertensive rats. Aqueous fraction was more potent and n-hexane the least. In isolated rat aortic rings precontracted with phenylephrine, crude extract induced endothelium-dependent effect. The endothelium-dependent component of vasodilatory effect was ablated with L-NAME, and denudation of endothelium. The aqueous fraction was most potent vasodilator. In aortic rings from hypertensive rats, extract and fractions produced partial endothelium-independent effect which was not affected by pretreatment with L-NAME, indicating endothelium dysfunction in the hypertensive rats and suggesting additional vasodilatory mechanisms. In rabbit aorta, the extract and fractions also inhibited phenylephrine and high K^+-induced precontractions, and shifted Ca^(++) concentration–response curves. Conclusions: Our findings indicate that extract and fractions of H. helix are antihypertensive remedies, which is the outcome of vasodilatory effect. This vasodilatory effect is mediated through nitric oxide and Ca^(++) antagonism.
基金Supported by Applied Basic Research Program of Yunnan Province(2017FD087)National Natural Science Foundation of China(31660559)
文摘Potted seedlings of Hedera nepalensis and H. helix were exposed to formaldehyde and low light alone or in combination for four months, before the morphological and nutritive indices of the plants were measured to assess the effects of formaldehyde and low-light stress on the growth and nutrient accumulation of Hedera species. The results showed that treatment with either formaldehyde or low-light stress reduced leaf length, leaf width, stem length, stem diameter, root length and biomass, but increased leaf shape index of the two Hedera species. Treatment with formaldehyde alone increased root-shoot ratio, while treatment with formaldehyde ＋ low light or low light alone decreased root-shoot ratio of the two Hedera species. Under the stress of formaldehyde, soluble sugar content decreased, while starch content increased in roots, stems and leaves of the two Hedera species; total carbon content, proline content and carbon-to-nitrogen （C/N） ratio increased, while protein content and phosphorus content decreased in roots of the two Hedera species; the contents of total carbon, protein and total nitrogen in leaves of the two Hedera species; C/N ratio in roots of H. nepalensis increased; the C/N ratio in roots of H. nepalensis and that in leaves of the two Hedera species significantly increased. Under formaldehyde ＋ low light stress, the soluble sugar content decreased, while starch content increased in stems and leaves of the two Hedera species; starch content in roots of the two Hedera species decreased; the contents of protein and total nitrogen decreased, while C/N ratio increased in leaves of H. nepalensis ; proline content in roots and stems of the two Hedera species significantly increased.
基金Tianjin Science and Technology Plan Project(18ZXBFNC00370)Industrial Innovation and Entrepreneurship Team Project of Hebei Province(199A2905H)Fund of Central Government for Guiding Science and Technology Development in Hebei Province(206Z6303G).
文摘[Objectives]The purpose was to establish an induction system for friable callus of Hedera nepalensis var.sinensis with different parts.[Methods]By screening the most suitable explant and adjusting the hormone ratio of medium,friable calli of H.nepalensis var.sinensis were induced.[Results]The calli could be induced from leaves,petioles and stem segments,but the ideal explant was stem segments,with induction rate reaching 98%.The optimal medium for callus proliferation was MS+0.5 mg/L KT+1.0 mg/L 2,4-D+30.0 g/L sucrose.After 3-4 generations of subculture on MS+0.5 mg/L BA+1.0 mg/L 2,4-D+30.0 g/L sucrose,favorable friable calli of H.nepalensis var.sinensis were obtained.[Conclusions]The friable calli induced in this experiment can lay a foundation for in-vitro regeneration and cellular secondary metabolite production of H.nepalensis var.sinensis.
